Transdisciplinary interventions for environmental sustainability.

This paper presents a case study of a transdisciplinary research based on an ex-post assessment of the environmental and socio-behavioral contexts of solid waste management in Lebanese peri-urban communities. Lessons learned are compiled into the Transdisciplinary Interventions for Environmental Sustainability conceptual framework. The approach starts with building a team of researchers and non-academic partners, continues with co-creating solution-oriented knowledge, and ends by integrating and applying the produced knowledge. The co-created knowledge includes the environmental and socio-behavioral ex-post assessment's results. The former reveals low air pollution levels, evidence of waste-related water contamination, and higher self-reported frequencies of ill-health symptoms and diseases closer to the landfill. The latter indicates that the community's perception about waste production differs from the real accounting of generated waste. Nine lessons are identified: (1) inherent common interest between the researchers and the community, (2) flexible interdisciplinary research team, (3) representative citizen committee, (4) contextually-informed outreach coordinator, (5) iterative research process accounting for the shifting socio-political context, (6) common expectations of the research process, (7) boundary objects leading to spin-off activities in the same setting, (8) effective communication strategy, and (9) ex-post assessment of subsequent societal and scientific impacts. The non-phased framework links all nine pointers in a logical order to ease scalability. The study answers a global need for a unified, clear, broadly adopted framework for transdisciplinarity and a deeper understanding of factors ensuring full-circle knowledge co-creation in waste-related contexts in the global South. The study offers managerial and research implications and suggests avenues for further research.

Citizen science in Lebanon-a case study for groundwater quality monitoring.

Over the past decade, several citizen science projects have been launched, with a smaller subset addressing citizen scientists' involvement in water quality monitoring. Most of these projects were conducted in developed countries and focused on qualitative assessment and measurements of a limited number of water quality parameters. Moreover, data generated by citizen scientists were mainly for monitoring purposes and rarely resulted in remedial measures. In this work, a collaborative citizen science approach involving local citizens and university researchers was applied to assess the groundwater quality in a Lebanese village. Using a mobile laboratory, winter and summer sampling campaigns were conducted and 12 physical, chemical and biological water quality parameters were tested. Results indicated that the data generated by the citizen scientists were comparable with those generated by university researchers for the majority of physical and chemical water quality parameters. However, the bacteriological test results showed a marked difference and may be attributed to the complexity of the testing procedure and quality of testing material. The collaborative and participatory approach resulted in building local capacity and knowledge and in the formation of a locally elected water committee which will be responsible for continuous monitoring of the groundwater resources.

Using stakeholder preferences to select native tree species for reforestation in Lebanon.

Using a diverse assemblage of suitable species for reforestation is necessary to enhance biodiversity and ensure resilient forest ecosystems. However, selection of diverse native species for reforestation is difficult, requiring consideration of the preferences of different stakeholders. In this study we identify species to be included in reforestation of an ecologically important watershed in North Lebanon based on ratings produced by stakeholders from Lebanon's public, private and academic sectors. Twenty-two tree species being produced in Lebanese nurseries were identified as ecologically suitable by experts. Stakeholders (n = 34) were asked to rate these 22 species according to conservation priority and ecological suitability in an online survey. Although there was a high degree of variability in ratings among respondents, those who identified as biodiversity-focused did not differ from those who identified as forestry-focused. Looking within the two foci, we found significant variability among forestry-focused respondents but not among biodiversity-focused respondents. Although there was no significant difference in ratings between biodiversity- and forestry-focussed respondents, the resultant rankings differed considerably. We also found significant variability in preferences within forestry-focussed but not biodiversity-focussed respondents. Weighting by respondents' knowledge of species had little effect on rankings. The variability in preferences between stakeholders, including the considerable within-group variability we found among forestry-focused respondents, highlights the importance of soliciting preferences from multiple stakeholders when selecting species to be used in reforestation efforts.

A content analysis of nature photographs taken by Lebanese rural youth.

'Living in Harmony with Nature' is a 2050 vision put forth by the Convention on Biological Diversity (CBD) which takes into consideration culture and locality of perceptions of nature and aspirations for its use. Considering that 54% of the world population lives in cities, where nature has been decimated, the role of rural communities, living within or in proximity of natural and semi natural areas, will effectively influence the fate of the 'remaining' nature while they engage in local development. Reconciling between growth and development on the one hand, and nature conservation on the other, necessitates an understanding of how rural communities, especially the youth, imprint their own ideas on landscapes and develop ownership over natural spaces. In order to extend the understanding of how harmony with nature is perceived in different parts of the world, this paper presents the findings of primary research involving a group of young people who live in rural areas in Lebanon, a country in the Arab Middle East. Participatory research based on photovoice methodology was conducted with 77 young people aged 7-16 and residing in five rural villages located in different parts of Lebanon. Photographs taken by participants indicated that for many, nature was not perceived literally i.e. woodlands, forests, plants, animals, etc…. Instead, the participants saw nature as part of agriculture and local culture. Nature was also seen as symbolic expression of the participants' inner state of mind. Narratives written to explain the photographs shed light on the perception of harmony with nature which focused on positive family experiences and relayed personal emotions, abstract, and holistic yet functional view of nature. Another recurring theme that emerged from photographs and narratives was the role of family members, in particular grandparents, as having a strong influence on the positive perceptions of nature.

Anti-Inflammatory and Cytostatic Activities of a Parthenolide-Like Sesquiterpene Lactone from Cota palaestina subsp. syriaca.

A sesquiterpene lactone 1-ß,10-Epoxy-6-hydroxy-1,10H-inunolide (K100) was isolated through "bioassay-guided fractionation" from Cota palaestina subsp. syriaca, an Eastern Mediterranean endemic plant. K100 inhibited endotoxin- (ET-) induced proinflammatory markers: IL-6, MMP-9, and NO in normal mouse mammary SCp2 Cells. Molecular docking in silico suggested that K100, having highly analogous structure as parthenolide (PTL), an anticancer compound, could bind PTL target proteins at similar positions and with comparable binding affinities. Both compounds, K100 and PTL, inhibited the proliferation and prolonged the S-phase of the cell cycle of breast adenocarcinoma MDA-MB-231 cells grown in 2D cultures. Noncytotoxic concentrations of K100 and PTL decreased the proliferation rate of MDA-MB-231 and shifted their morphology from stellate to spherical colonies in 3D cultures. This was accompanied with a significant increase in the amount of small colonies and a decrease in the amount of large colonies. Moreover, K100 and PTL decreased cellular motility and invasiveness of MDA-MB-231 cells. In summary, these results suggest that K100 exhibits PTL-analogous anti-inflammatory, cytostatic, and antimetastatic effects.

Sesquiterpene lactones isolated from indigenous Middle Eastern plants inhibit tumor promoter-induced transformation of JB6 cells.

BACKGROUND: Sesquiterpene lactones (SL) are plant secondary metabolites that are known for their anti-fungal, anti-bacterial, anti-inflammatory, and anti-tumor properties. Considering that several SL-derived drugs are currently in cancer clinical trials, we have tested two SL molecules, 3-ß-methoxy-iso-seco-tanapartholide (ß-tan) isolated from Achillea falcata and salograviolide A (Sal A) isolated from Centaurea ainetensis, for their anti-tumor properties. We used the mouse epidermal JB6P + cells as a model for tumor promotion and cellular transformation. Key players that are involved in cellular transformation and tumorigenesis are the AP-1 and NF-κB transcription factors; therefore, we assessed how ß-tan and Sal A modulate their signaling pathways in JB6P + cells. METHODS: The effects of ß-tan and Sal A on the growth of normal and neoplastic keratinocytes and on the tumor promotion-responsive JB6P + cells were determined using the MTT assay. Anchorage-independent cell growth transformation assays were used to evaluate the anti-tumor promoting properties of these SL molecules in JB6P + cells and dual luciferase reporter assays and western blot analysis were used to investigate their effects on tumor promoter-induced AP-1 and NF-κB activities and protein levels of key AP-1 and NF-кB target genes. RESULTS: ß-tan and Sal A selectively inhibited tumor promoter-induced cell growth and transformation of JB6P + cells at concentrations that do not affect JB6P + and primary keratinocytes basal cell growth. In addition, both molecules reduced basal and tumor promoter-induced NF-κB transcriptional activities, differentially regulated basal and tumor promoter-induced AP-1 transcriptional activities, and modulated key players of the AP-1 and NF-κB signaling pathways. CONCLUSIONS: These results highlight the anti-tumor promoting properties of ß-tan and Sal A. These SL molecules isolated from two plant species native to the Middle East may provide opportunities for complementary medicine practices.

Effects of different irrigation, drying and production scenarios on the productivity, postharvest quality and economic feasibility of Origanum syriacum, a species typically over-collected from the wild in Lebanon.

BACKGROUND: The potential of Origanum syriacum L. to become a cultivated crop in Lebanon rather than being harvested from the wild was investigated at the production, postharvest and economic levels. Three irrigation schemes were tested on growth parameters of cultivated oregano plants. In order to identify the most quality-preserving drying technique, air-, oven (30 °C)- and freeze-dried oregano samples were assessed through sensory analysis. Cost/benefit analysis was used to compare the economic potential of O. syriacum in four production scenarios. RESULTS: An irrigation scheme of 16 L per plant administered every 2 weeks (medium irrigation) during the dry period resulted in a doubling of the horticultural (56.34%) and hydro-distilled essential oil (55.22%) yields. Descriptive sensory analysis showed that air-dried material had the desired flavour, aroma and colour attributes. Net present values ranged from - 677 406.02 to 562 301.5 US$ and benefit/cost ratios from 0.5341 to 5.1993 for a 1 ha, 5 year life-cycle oregano farm. CONCLUSION: Irrigating plants once every 2 weeks during the dry season optimised herbage and essential oil yields of oregano, while air drying of plant material preserved its organoleptic attributes as a spice. Three of the four production scenarios, namely Zaatar production, herbal tea and Zaatar production and essential oil production, were economically feasible, with the last of these having the highest economic feasibility.

Screening of selected indigenous plants of Lebanon for antimicrobial activity.

The objective of this study is to test in vitro the antimicrobial efficacy of 39 water and 39 methanol extracts derived from different parts of 27 indigenous wild plant species that have been commonly used in Lebanese folk medicine. The antimicrobial efficacy was determined using the single disk diffusion method, with 10 and 20 microl load extract volume per disc. Nine test microorganisms were used namely, Escherichia coli, Proteus sp., Pseudomonas aeruginosa, Shigella dysenteria, Salmonella enteritidis, Salmonella typhi, Staphylococcus aureus, Streptococcus faecalis, and Candida albicans. Only one water extract out of 39 derived from whole plant of Alchemilla diademata showed an antimicrobial activity against Staphylococcus aureus. The percentage of test organisms that were susceptible to 10 most efficacious methanol plant extracts (20 microl/disc) were as follows: Achillea damascena whole plant (88.8%), Anthemis scariosa flower (88.8%), Cirsium sp. whole plant (88.8%), Centaurea ainetensis flowers (88.8%), Hieracium sp. whole plant (88.8%), Origanum libanoticum whole plant (99.9%), Ranunculus myosuroudes whole plant (88.8%), Nepata curviflora leaf (88.8%), Nepata curviflora stem, and Verbascum leptostychum flower (99.9%). The minimum inhibitory concentration (MIC) was determined on plant extracts that showed high efficacy against the test organisms. The chance to find antimicrobial activities was more apparent in methanol rather than water extracts of the same indigenous plants of Lebanon, with higher antimicrobial activities in 20 microl methanol extract-discs in comparison to that present in the 10 microl discs (P < 0.05).