RESUMO
Repair of UV-light induced DNA damage and changes in the semiconservative DNA synthesis were studied by in vitro autoradiography in the skin of patients with lightdermatoses (polymorphous light eruption, porphyria cutanea tarda, erythropoietic protoporphyria) and xeroderma pigmentosum as well as in that of healthy controls. In polymorphous light eruption the semiconservative DNA replication rate was more intensive in the area of the skin lesions and in the repeated phototest site, the excision repair synthesis appeared to be unaltered. In cutaneous porphyrias a decreased rate of the repair incorporation could be detected. Xeroderma pigmentosum was characterized by a strongly reduced repair synthesis.
Assuntos
Reparo do DNA , Transtornos de Fotossensibilidade/metabolismo , Adulto , Autorradiografia , DNA/biossíntese , Humanos , Pessoa de Meia-Idade , Transtornos de Fotossensibilidade/fisiopatologia , Porfirias/metabolismo , Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversosRESUMO
Occurrence of lymphocytotoxic antibodies of the "cold type" was studied in 92 cases with various lightdermatoses. They were detected in the sera of 39% of the patients with cutaneous porphyrias and never in cases with polymorphous light eruption and other photodermatosis. Correlations between their presence and the duration of the porphyria as well as the severity of hepatopathy could be observed. The results indicate the importance of a long-standing tissue damage in the production of these antibodies. In addition the findings confirm the hypothesis according to which polymorphous light eruption does not belong to the lupus erythematosus entity.
Assuntos
Citotoxicidade Celular Dependente de Anticorpos , Linfócitos/imunologia , Transtornos de Fotossensibilidade/imunologia , Porfirias/imunologia , Testes Imunológicos de Citotoxicidade/métodos , Humanos , Imunidade Celular , Imunoglobulinas/isolamento & purificaçãoRESUMO
The proportions of T-B cells of peripheral lymphocytes in DLE patients as well as the effect of chloroquine treatment on T cell count were studied. In DLE, both the active and the total rosette-forming cell counts decreased, whereas the B cell count did not differ from normal values. Long-term chloroquine administration causes a further reduction in T cell count.
Assuntos
Linfócitos B/imunologia , Cloroquina/uso terapêutico , Lúpus Eritematoso Discoide/imunologia , Linfócitos T/imunologia , Humanos , Reação de Imunoaderência , Contagem de Leucócitos , Lúpus Eritematoso Discoide/tratamento farmacológicoRESUMO
The pathogenetic role of liver damage in photodermatoses induced by oral contraceptives was investigated. From among 121 cases with photodermatosis, it was in 4 cases of polymorphic light eruption-like dermatosis and in 2 cases of porphyria cutanea tarda that a longterm use of antibaby pills preceded the development of skin disease. Physical and biochemical studies of the liver of the patients suggested that the hepatotoxicity of the estrogen component of oral contraceptives can play a role not only in the pathomechanism of porphyric cases, but also in that of cases occurring with the clinical picture of polymorphic light eruption.
