Assessment of Knowledge, Attitude and Practice (KAP) Regarding Antimicrobial Usage and Resistance Among Animal Health Professionals of East Wallaga Zone, Oromiya, Ethiopia.

Background: Antimicrobial resistance (AMR) is one of the top global public health and economic threats. The use of antimicrobials (AMs) in animal production is a major contributor to the development of AMR globally. Animal health professionals (AHPs) play a key role in ensuring judicious use of AMs. Objective: To assess the knowledge, attitude and practice (KAP) of antimicrobial usage (AMU) and AMR among healthcare professionals in Nekemte town, Leka Dulecha and Sibu Sire districts. Methods: A cross-sectional study was conducted with 120 purposively chosen AHPs residing in the districts and the town. A semi-structured questionnaire consisting of 49 questions was used to ascertain the KAP. The chi-square test (X2) was used to analyze the association between the knowledge score and demographic profile of the study participants. Results: In the study the overall knowledge of the participants was moderately appreciable, and all participants had positive attitudes toward AMR and appropriate usage. In terms of knowledge of antibiotic use, the majority (93.33%) of the participants correctly answered the statement that antibiotics can kill viruses. About 84.17% of the participants correctly knew that antibiotics killed or stopped the growth of both bad and good bacteria. The majority of the participants (74.17%) always or (25.83%) sometimes rely on usage of antibiotics without a doctor's prescription. It was shown that comparing respondents from Sibu Sire, Leka Dulecha and Nekemte town, the scores of knowledge of AMU were significantly (X2=14.13, p=0.007) different. Most animal healthcare professionals from the Sibu sire have a good knowledge of AMU, and contribute to AMR development. Conclusion: The study revealed that there was moderate knowledge and positive attitude toward AMU and resistance. This warrants continuing capacity building programs for the professionals on AM usage and resistance, and development of field-friendly disease diagnosis and management tools is essential in the need to reduce AMR.

Seroprevalence and Associated Risk Factors of Mycoplasma gallisepticum Infection in Poultry Farms of Hawasa and Bishoftu, Central Ethiopia.

Background: Mycoplasma gallisepticum (MG) infection is an economically important respiratory disease in the poultry production system worldwide. It is characterized by rapid transmission and causing many obstacles to poultry industries in different parts of Ethiopia. Methods: A cross-sectional study was employed from January to September 2021 to estimate the seroprevalence and associated risk factors of MG in domestic layer chickens in large and small commercial poultry farms in Hawassa and Bishoftu area, Ethiopia. A total of 368 blood samples were collected. Data were analyzed using SPSS version-20, reported as percent prevalence, and Pearson's chi-square was used to assess the association between factors considered to have association with MG infection. The samples were processed by using an indirect ELISA (ProFLOKIBV, USA) test coated with antibody against MG. Results: The total seroprevalence of MG infection was found to be 70.65%. Significant variation in environmental risk factors with seroprevalence was assessed and the higher prevalence of MG was significantly (χ 2 = 14.42; p < 0.05) higher in layer chicken farms found in Bishoftu. Likewise, it was significantly observed in the adult chicken and commercial production system. There were significant difference between breeds and ages of birds with the occurrences of MG (χ 2 = 19.60 and χ 2 = 17.46, respectively). Management related risk factors found around the types of farms were significantly different with the occurrences of MG (OR = 52.5; p < 0.05). Conclusion: The evidence from seroprevalence of the MG infection in the current finding provides an indication of the eminence of infection in the study areas. Moreover, it provides an insight on the prevalence of MG infection and further molecular characterization of the organism needs to be conducted in the areas. Biosecurity measures combined with vaccination and sero-monitoring should also be implemented in the farms.

Seroprevalence of Infectious Bronchitis Virus in Broiler and Layer Farms of Central Ethiopia.

Background: Infectious bronchitis virus (IBV) is a highly contagious viral disease of chicken typically affecting the reproductive and respiratory tract and results in possible economic causes from its serious infectious and transmission characteristics. Methods: A cross-sectional study was carried on serum samples of chickens selected from six (two commercial and four small holder) farms and two types of production (broiler and layer) to detect seroprevalence of IBV and its associated risk factors in Bishoftu and Holeta areas of central Ethiopia from June 2021 to September 2021. A total of 354 blood samples were collected and subjected to indirect ELISA test by IBV antibody test kit ((ProFLOK IBV), from ProFLOK Laboratories Inc., (USA)) to detect specific antibodies against IBV. Results: Overall, 97.46% seroprevalence was identified. From 230 and 124 samples collected from commercial and smallholder poultry farms, 226 (98.26%) and 119 (95.98%) positive results were obtained, respectively. Among the production types of chickens, high seroprevalence (99.31%) was found in layer poultry, and lower seroprevalence (96.17%) was found in the case of broiler chicken. Significant association was observed among different associated risk factors particularly age, sex, breed, and production types of chickens. From the tested chickens, all age groups, species, and farm types have high seroprevalence of IBV. The prevalence of IBV was highly significant (p ≤ 0.01) in the study site. The risk factors indicated could have increased infection prevalence, pathogens' economic impact, and disease occurrence. Conclusion: IBD is complicating factor affecting poultry production systems in the area. Vaccine and biosecurity measures are recommended for the control of IBV. Furtherly, identification and characterization (by using RT-PCR) of persistent serotype of IBV circulating in the field are recommended.

Knowledge, Attitude, and Practice Toward Pet Contact Associated Zoonosis in Western Ethiopia.

BACKGROUND: Households consider their dogs and cats as their close friends. They act as companion animals. The contact between pets and their owners results in transmission of zoonotic disease. In Ethiopia, dogs and cats are the most abundant carnivores, and diseases associated with them affect wide parts of the community. There is limited knowledge, practice, and attitude within the communities toward pet contact associated zoonotic disease. METHODS: A community-based semi-structured questionnaire complemented with an interview was delivered to 633 household pets' owners in Sibu Sire, Jimaa Arjo, and Wayu Tuqa districts in Western Ethiopia to evaluate knowledge, attitude, and practices toward pet contact associated zoonotic disease. RESULTS: Socio-demographically, 54.6% of the study participants were female. According to this finding concerning common pet contact zoonotic disease, 70% of the respondents had knowledge about rabies, with Echinococcosis, toxoplasmosis, and ring worm also being commonly heard of. Contamination of feed and water (21.71%) and animal bites (21.01%) are the predominant modes of transmission, whereas animal waste, fecal oral route, and touching pets are also common ways for disseminating zoonotic disease. The dominant symptoms noted by respondents were behavioral change, depression, lack of appetite, itching, and diarrhea. In all attitude-related responses, there was a significant association (p<0.05) between the number of respondents and the variable studied. There was also a statistically significant association of KAP score (p<0.05) with educational rank and the work of respondents. CONCLUSION: This study indicates the importance of pet ownership to the community, which is also associated with transmitting different zoonotic diseases. Moreover, there are inconsistencies on regular veterinary use, pet management, and proper prevention and treatment measures of the disease. Coordinated efforts are expected from different stakeholders in enhancing community KAP level towards pet contact associated zoonosis.

Seroprevalence and Associated Risk Factors of Infectious Bursal Disease in Chickens Managed Under Intensive and Backyard Production Systems in Western Oromia, Ethiopia.

BACKGROUND: Infectious bursal disease (IBD) is a highly contagious viral disease challenging poultry industry throughout the world. It is also among the main obstacles in the different chicken production system in Ethiopia. This study was conducted with the aim of determining seroprevalence and associated risk factors of IBD in backyard and intensively managed chickens of the study area. METHODS: A cross-sectional study was conducted in selected districts of western Oromia on intensive and backyard production systems to investigate seroprevalence of IBD and associated risk factors from September 2020 to August 2021. A total of 384 chickens of either sex, 128 from each district, were included from three randomly selected peasant associations (PAs). In addition, 180 individual poultry keepers, of both sexes, and different educational backgrounds, were included for questionnaire survey (Supplementary Material). Data were analyzed using SPSS Version-20, and results were presented as percentages. The samples were processed by using (ProFLOKIBV, USA) indirect enzyme-linked immunosorbent assay (I-ELISA) kits. RESULTS: Out of 384 serum samples tested, 66.93% (n = 257) were found positive by indirect ELISA. The highest prevalence was recorded from Sasiga district Balo Bareda PA. Prevalence of IBD showed significant statistical association (p < 0.05) among owner education level, chicken rearing practice, origin and breed. Questionnaire survey results showed the majority of respondents lack sufficient knowledge about IBD and other chicken diseases. The practice of backyard chicken producers on vaccination and treatment of sick birds is limited. CONCLUSION: In conclusion, IBD is a major factor hindering production and health of birds. Proper vaccination programs coordinated with awareness creation of chicken owners on how to isolate and treat sick birds should be implemented.