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2.
Exp Anim ; 69(4): 423-429, 2020 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-32554941

RESUMO

The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice.


Assuntos
Animais Geneticamente Modificados , Criopreservação/métodos , Transferência Embrionária/métodos , Embrião de Mamíferos , Camundongos/embriologia , Manejo de Espécimes/métodos , Meios de Transporte/métodos , Animais , Vitrificação
3.
Tokai J Exp Clin Med ; 37(1): 6-10, 2012 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-22488556

RESUMO

Chromomycosis is a chronic fungal disease of the skin and subcutaneous tissues caused by a group of dematiaceous black fungi. Small lesions can be removed with excision, but other cases are difficult to treat. We report a case of chromomycosis caused by Fonsecaea pedrosoi (F. pedrosoi). The case involved a 74-year-old man, who had noted a lesion on the back of the right thigh, that was gradually enlarging and reaching up to 30 cm in diameter, in 20-years. From microscopic examination, sclerotic cells were seen. We diagnosed this case as chromomycosis caused by F. pedrosoi on mycological examination. The patient was initially treated with oral terbinafine (250 mg/day) as the lesion was very large. After the 18 months treatment, the size of the lesion reduced to 1 cm, then the remaining lesion was excised.


Assuntos
Antifúngicos/uso terapêutico , Cromoblastomicose , Naftalenos/uso terapêutico , Idoso , Antifúngicos/administração & dosagem , Ascomicetos/isolamento & purificação , Cromoblastomicose/tratamento farmacológico , Cromoblastomicose/microbiologia , Cromoblastomicose/cirurgia , Terapia Combinada , Humanos , Masculino , Naftalenos/administração & dosagem , Terbinafina , Resultado do Tratamento
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