Causal relationship between sleep disorders and the risk of Alzheimer's disease: A Mendelian randomization study.

BACKGROUND AND OBJECTIVE: Epidemiological studies have shown that sleep disorders are risk factors for Alzheimer's disease (AD), but the causal relationship between sleep disorders and AD risk is unknown. We aim to assess the potential genetic causal association between sleep characteristics and AD, which may contribute to early identification and prediction of risk factors for AD. METHODS: Seven sleep-related traits and the outcome phenotype AD were selected from published genome-wide association studies (GWASs). These sleep-related characteristics and instrumental variables (IVs) for AD were extracted. Two-sample and multivariate Mendelian randomization (MR) analyses were performed to assess the causal relationships between sleep characteristics and AD. The inverse variance weighted (IVW), weighted median (WME), weighted mode (WM), MR-Egger regression (MR-Egger) and simple mode (SM) models were used to evaluate causality. The existence of pleiotropy was detected and corrected by MR-Egger regression, MR pleiotropy residuals and outliers. RESULTS: A two-sample MR study revealed a positive causal association between sleep duration and the onset of AD (OR = 1.002, 95 % CI: 1.000-1.004), and the risk of AD increased with increasing sleep duration. The MR-Egger regression method and MR-PRESSO were used to identify and correct pleiotropy, indicating that there was no horizontal pleiotropy. Heterogeneity was evaluated by Cochran's Q, which indicated no heterogeneity. In a multivariate MR study with seven sleep characteristics corrected for each other, we found that sleep duration remained causally associated with AD (OR = 1.004, 95 % CI: 1.000-1.007). Moreover, we found that after mutual correction, daytime napping had a causal relationship with the onset of AD, and daytime napping may reduce the risk of AD (OR = 0.995, 95 % CI: 0.991-1.000). CONCLUSION: This study is helpful for the early identification and prediction of risk factors for AD, long sleep durations are a risk factor for AD, and daytime napping can reduce the risk of AD.

Occurrence and ecological risks of flonicamid and its metabolites in multiple substrates from intensive rice-vegetable rotations in tropical China.

Rice-vegetable rotations are dominant in (sub)-tropical agriculture worldwide. However, fate and risks of the insecticide flonicamid (FLO) and its main degradates (collectively called FLOMs) in multiple substrates from those cropping systems remain largely unknown. In this study, we characterized residual concentrations, driving factors, transport, and potential ecological risks of FLOMs in different substrates in 28 tropical rice-vegetable rotations. Concentrations (median) of FLOMs were 0.013-3.03 (0.42) ng g-1 in plants, 0.012-1.92 (0.23) ng g-1 in soil, 0.029-0.63 (0.126) µg L-1 in water, and 0.002-0.398 (0.055) ng g-1 in sediments. Flonicamid and its metabolite N-(4-trifluoromethylnicotinoyl) glycine were the dominant species in the four substrates (84.1 % to 88.5 %). Plants had the highest levels of FLOMs, with the highest bioconcentration factor in peppers. According to boosted regression trees coupled with a partial least squares structural equation model, levels and composition of FLOMs showed high spatiotemporal and crop-related patterns in different substrates, with patterns highly codetermined by agricultural practices (e.g., crop type and FLO/neonicotinoid/pyrethroid applications), substrate parameters (e.g., pH, organic matter or total organic carbon), and climate features (e.g., wet/dry seasons). Moreover, a fugacity method indicated differences in transport and partitioning patterns in different substrates during rice and vegetable planting periods. Integrated substrate risk assessment of FLOMs contamination was conducted based on species-sensitive distributions and substrate weight index. Although overall risks of FLOM contamination in tropical rice-vegetable rotations were negligible to low, the highest risks were in soils, vegetable planting periods, and a central intensively planted area.

Neonicotinoids in draining micro-watersheds dominated by rice-vegetable rotations in tropical China: Multimedia occurrence, influencing factors, transport, and associated ecological risks.

Multimedia contamination by neonicotinoid (NEO) residues has attracted global attention. However, data regarding the multimedia polluted status under certain typical cropping scenarios and the associated risks are scarce. Here, the multimedia occurrence, spatiotemporal distribution, driving factors, transport, and ecological risks of NEOs from tropical rice-vegetable rotation fields were characterized. The heavy NEOs resided in multiple media, and imidacloprid and acetamiprid were the prevailing NEOs, with concentration contributions of 65-80%. The pollution levels of the NEOs, rather than their compositions, exhibited significant spatiotemporal heterogeneity and were highly correlated with the collective (agricultural practices and climate conditions) and differential (e.g., media properties) factors identified using an auto linear regression model. Furthermore, the multimedia transport of NEOs was largely similar but non-negligibly different during the rainy and dry seasons. A new multimedia ecological risk assessment revealed that 50.6% sites were at high risk, and the risk hotspots occurred in the central areas and the winter planting period. The risks were largely contributed by imidacloprid and thiamethoxam, indicating that there were non-ignorable ecological risks. Our results highlight the differential pollution patterns (distribution, transport, and driving factors) of the prevailing NEOs under tropical agricultural scenarios, and the fact that special attention should be paid to the risks posed by NEOs.

Occurrence, distribution, and driving factors of current-use pesticides in commonly cultivated crops and their potential risks to non-target organisms: A case study in Hainan, China.

Multiple pesticides are heavily applied in crops grown in China's tropics due to the prevalence of diseases and pests, thus posing potential risks to nontarget organisms (e.g., honeybees, lacewings, ladybugs, and humans). However, there is little information on this topic. This study is the first assessment of the occurrence, driving factors, and ecological/human health risks of 32 current-use pesticides (CUPs) in 10 frequently-planted crops collected from practicing rice-vegetable rotation systems in Hainan, China. Of the 132 whole crop samples, 44 (33.3 %) residues from ≥8 pesticides were detected in 9.09 % of crop samples with concentrations ≥0.5 mg kg-1. Six pesticide residues, namely carbendazim, pyraclostrobin, acetamiprid, thiophanate methyl, phoxim, and imidacloprid, were detected in 72.7 % of samples, with concentrations ranging from 0.0021 to 13.5 (median = 0.032) mg kg-1. Among them, carbendazim, pyraclostrobin, and acetamiprid were the most common, contributions from 10.2 to 25.5 % and a detection frequency ranging from 25.6 to 56.1 %. The order of total concentration of 32 CUPs (∑32 CUP) concentrations during the year was January > May > November > August and vegetables > rice, being highly related with pesticides usage pattern, crop type, plant accumulation/dissipation and plant lipid contents. The ecological risk quotients (RQs) to four beneficial terrestrial organisms showed that 9.6-39.1 % of samples posed a potential medium or high ecological risk, with 13.6-65.9 % of samples at ∑RQ > 1 being highly affected by the residues of neonicotinoids and emamectin benzoate. Emamectin benzoate (8.9 %) and acetamiprid (5.6 %) exceeded the individual Maximum Residue Levels based on Chinese legislation (GB2763-2021). Moreover, cumulative dietary exposure presented a higher risk to humans in 11.0 and 22.0 % of the cases for acute and chronic, mainly originating from the higher concentration contributors of systemic pesticides in edible crops. Therefore, the regulation and monitoring of CUP residues is imperative for rice-vegetable rotation systems in tropical China to avoid negative effects on nontarget organisms.

Polycyclic aromatic hydrocarbons (PAHs) in surface soils of tropical reef islands in China under external plant and soil introduction: Occurrence, sources, risks, and relationships with soil properties, vegetation cover, and soil source.

This study explored the levels, sources, and risks of PAHs in soils from Yongle Atoll (YLA) and Xuande Atoll (XDA) of the Xisha Islands (XSIs) in the South China Sea, China, under different vegetation cover types and soil sources. The results clearly showed that the levels of 16 US EPA priority PAHs (Σ16PAHs) are relatively low in XDA and YLA, with concentrations ranging from not detected (ND) to 151 ng/g (average 15.7 ng/g) and ND to 5.8 ng/g (average 2.1 ng/g), respectively. Three- and four-ring PAHs (62.3% and 53.8%) were widely distributed in YLA and XDA. The average concentration of Σ16PAHs in soils with shrub cover was 1.4, 1.8, 4.8, and 5.0 times higher than that in soils with herbaceous cover, vegetable cover, arbor cover, and no plant cover, respectively. Source analysis using binary diagnostic ratios and the positive matrix factorization (PMF) model suggested that PAHs have similar sources (gasoline/coal combustion, coke production, and biomass combustion), but different contributions in native soil and introduced soil. Moreover, diesel-related vehicular emission was identified to be an additional source of PAHs in native soil. Pearson's correlations revealed strong relationships between PAHs and organic matter or total organic carbon. The cancer risk of PAHs varied among different vegetation cover types and soil sources, following the orders herbaceous cover > vegetable cover > shrub cover > arbor cover > no plant cover and introduced soil > mixed soil > native soil. Nevertheless, the risk remained lower than the risk threshold (10-6), suggesting low carcinogenesis risk in the two atolls. Our findings provide new evidence for the introduction of external vegetation/soil acting as a driver of changes in the characteristics of PAHs in islands, and also underline the negligibility of the PAH increase in soils in the South China Sea, China, from the perspective of health hazards.

Pesticides in surface waters of tropical river basins draining areas with rice-vegetable rotations in Hainan, China: Occurrence, relation to environmental factors, and risk assessment.

Pesticides are heavily applied in rice-vegetable rotations in tropical China, yet publicly available information on the contamination and risk of currently used pesticides (CUPs) and legacy pesticides (LPs) in surface waters of river basins draining these areas is very limited. Therefore, in two tropical river basins (Nandu River and Wanquan River basins) dominated by rice-vegetable rotations in Hainan, China, pesticides were analyzed in 256 surface water samples in wet and dry seasons. Forty-one pesticides were detected, and total concentrations ranged from not detectable to 24.2 µg/L. Carbendazim and imidacloprid were the two most prevalent CUPs, detected in 59.8% and 17.7%, respectively, of surface water samples at concentrations above 0.1 µg/L. Chlorpyrifos was the main LP, detected in 9.0% of samples at a concentration above 0.05 µg/L. The fungicides difenoconazole and emamectin benzoate, the herbicide butachlor, and the insecticide acetamiprid occurred in ≥12.5% samples at concentrations above 0.1 µg/L. Surface waters typically (85.2%) contained 5 to 15 residues, with an average of nine. Seasonally, the concentrations of the 41 pesticides were in the order January > July > November > September. Spatially, the composition of the main CUPs (not LPs) was significantly different depending on position in the drainage, which also changed with seasons. Crop and pest types and wet and dry seasons were the key factors controlling the spatiotemporal distribution of CUPs and LPs in surface waters. On the basis of evaluations of the exposures to individual pesticides and the dominant combinations with ≥8 pesticides, multiple pesticides were likely a significant risk to aquatic organisms, although noncarcinogenic and carcinogenic risks to humans were low. This study provides valuable data to better understand pesticide occurrence and ecological risks in river basins draining areas with rice-vegetable rotation systems in tropical China.

Pesticide residues in agricultural topsoil from the Hainan tropical riverside basin: Determination, distribution, and relationships with planting patterns and surface water.

Pesticide residues were assessed in rice-vegetable rotation topsoil from tropical riverside basin. The 256 tropical topsoil samples from the Nandu River Basin (NRB) and Wanquan River Basin (WRB) in Hainan (China) were taken from 2018 to 2019. A total of 32 current-use pesticides (CUPs) and nine legacy pesticides (LPs) were detected using a method based on a QuEChERS technique. The highest residues were from the fungicide carbendazim (257.2 µg/kg) and the insecticide imidacloprid (139.4 µg/kg). The nine LPs (8 organochlorine pesticides and chlorpyrifos) were detected in 84.4% of the samples with mean concentrations ranging from 0.02 to 675.4 µg/kg. The two most commonly detected pesticides were imidacloprid and emamectin benzoate with detection rates of 96.8% and 91.8%, respectively. The highest total concentration of pesticides from the NRB and WRB was detected in July. The highest average number of multi-residues (16) was recorded in January, compared to 14 and 12 in July and November, respectively. There was a significant correlation between the multiple pesticide residues in soil and vegetable planting types (p < 0.01). There was a significant positive correlation between the pesticide number in topsoil and the pesticide number in water (p < 0.01). The multiple pesticide residues found in surface water were consistent with those in topsoil, suggesting a potential risk of water contamination in the rice-vegetable rotation.

The implications of water extractable organic matter (WEOM) on the sorption of typical parent, alkyl and N/O/S-containing polycyclic aromatic hydrocarbons (PAHs) by microplastics.

Microplastics sorption of persistent organic pollutants (POPs) was the core processes that cause negative effects to biota, and their influencing factors and related mechanisms are poorly understood. In this study, we explored the impacts of water extractable organic matter (WEOM), an important source of endogenous dissolved organic matter in mangrove sediment, on the sorption coefficients of typical parent, alkyl and N/O/S-containing polycyclic aromatic hydrocarbons (PAHs) by microplastics. The presence of L-WEOM (D) impeded the PAHs sorption as the coefficients (Kf) decreased to 10.17 (µg/kg)/(µg/L)n and to 8.39 (µg/kg)/(µg/L)n for fluorene (Flu) and 1-methyl-fluorene (1-M-Flu), respectively. The Kf exhibited good linear relationships with the aliphaticity of L-WEOM (p < 0.05) rather than the aromatic carbon/alkyl carbon content (p > 0.05). Under the presences of L-WEOM (D), (S) and (K), the lone pair electrons of N/O/S-containing PAHs was the dominant factor contributing to the obvious difference of the Kf values from the other groups. Moreover, the largest impact of L-WEOM (D) on the Flu sorption was in the case of PVC microplastics, while almost no effect was in the case of PS microplastics. The findings of our work may be helpful in improving our understanding of the role of WEOM on the sorption of PAHs to microplastics in the field mangrove sediment.

The retention and distribution of parent, alkylated, and N/O/S-containing polycyclic aromatic hydrocarbons on the epidermal tissue of mangrove seedlings.

The polycyclic aromatic hydrocarbons (PAHs) located on the epidermal tissues showed distinctive toxic effects to root, while the retention and distribution of PAHs on mangrove seedlings poorly understood. Our results confirmed that the partition coefficients (Kf) of the PAHs retained on the epidermal tissue of mangrove roots, such as Kandelia obovata, Avicennia marina and Aegiceras corniculatum, were much higher than the Poaceae plants roots, for example wheat and maize (Wild et al., 2005). Moreover, to the parent and alkyl PAHs, a well negative correlation was observed between the surface polarity of these three species of mangrove root and the Kf values (p < 0.05). To the N/O/S containing PAHs, these relationships were not obviously due to existing of the π-π, n-π interactions and hydrogen bonding between the N/O/S-containing PAHs and epidermal tissues. The PAHs retained on these three species of mangrove root epidermal tissues formed larger clusters than that of on Poaceae plants, such as wheat and maize (Wild et al., 2005) due to the limitation of the suberization of the root exodermis and endodermis. After exposure of 30 d, rhizo- and endophytic bacteria degraded parts of the N/O/S-containing PAHs to medium-lifetime fluorescence substances. To our knowledge, this is the first time to assess the retention of PAHs on the epidermal tissue of mangrove root, which will improve our understanding of the root uptake PAHs process.

In situ quantitative and visual investigation of the retention of polycyclic aromatic hydrocarbons on the root surface of Kandelia obovata using a microscopic fluorescence spectral analysis method.

A novel approach for in situ determination of individual benzo[a]pyrene (B[a]P), pyrene (Pyr) and anthracene (Ant) on the root surface micro-zone (0.960mm2) of Kandelia obovata (K. obovata) was established using a microscopic fluorescence spectral analysis (MFSA) system. The detection limits for the three polycyclic aromatic hydrocarbons (PAHs) were 44.2, 59.7 and 36.3ngg-1 on lateral roots and were 42.8, 62.4 and 39.1ngg-1 on taproots of the K. obovata root micro-zone. Using the established MFSA method, retention of the PAHs on the K. obovata lateral root and taproot surface micro-zone were investigated in situ. The retention of PAHs on the lateral root and taproot surface of K. obovata showed uneven distribution, and both of the retained quantities showed significant differences, which was related to both passive uptake patterns or active uptake patterns of the PAH and the polarity index ((O+N)/C) of the root surface. In addition, increased quantities of retention of the PAHs on both lateral root and taproot surfaces of K. obovata were observed in the order of Ant