RESUMO
A good correlation between fifty-percent inhibitory concentration (IC(50)) of hybrid liposomes (HL) composed of dimyristoylphosphatidylcholine and polyoxyethylene(n) dodecyl ether on the growth of MOLT-4/IIIB cells (MOLT-4 cells chronically infected with human immunodeficiency virus (HIV)) and the membrane fluidity of HL was obtained. Furthermore, the huge enhancement of virus production was observed in the latently HIV-infected (J(22)-HL-60) cells after the treatment with HL.
Assuntos
Membrana Celular/virologia , Infecções por HIV/tratamento farmacológico , HIV-1/metabolismo , Lipossomos/uso terapêutico , Animais , Linhagem Celular Tumoral , Membrana Celular/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Química Farmacêutica/métodos , Desenho de Fármacos , Células HL-60 , Humanos , Técnicas In Vitro , Concentração Inibidora 50 , Lipossomos/química , Fluidez de Membrana , Modelos Biológicos , Modelos QuímicosRESUMO
A procyanidin-rich extract from French maritime pine, Pycnogenol(R) (PYC), is known as an antioxidant that exerts a variety of physiological activities and is widely used in human beings. We report here that PYC inhibits not only human immunodeficiency virus type-1 (HIV-1) binding to host cells, but also its replication after entry in susceptible cells in vitro. Prominent biochemical alterations induced by PYC were the elevated expression of an intracellular antioxidant protein, manganese superoxide dismutase (Mn-SOD), and the inhibition of phosphorylation of the ribosomal S6 protein. Interestingly, ectopic expression of Mn-SOD inhibited HIV-1 replication as well. Inhibition of HIV-1 replication associated with induced expression of Mn-SOD in cells treated with PYC suggests the potential of this natural antioxidant inducer as a new anti-HIV-1 agent.
