RESUMO
The convergent synthesis of the dansyl-labeled probe of the thiophene-3-carboxamide analogue of annonaceous acetogenins, which shows potent antitumor activity, was accomplished by two asymmetric alkynylations of the 2,5-diformyl THF equivalent with an alkyne having a thiophene moiety and another alkyne tagged with a dansyl group. The growth inhibitory profiles toward 39 human cancer cell lines revealed that the probe retained the biological function of its mother compound, and would be useful for studying cellular activity.
Assuntos
Acetogeninas/química , Acetogeninas/farmacologia , Antineoplásicos/farmacologia , Compostos de Dansil/química , Tiofenos/química , Acetogeninas/síntese química , Antineoplásicos/síntese química , Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Relação Estrutura-AtividadeRESUMO
Five novel acetogenin analogues with a furan, thiophene, or thiazole ring were synthesized, and their inhibitory activities toward human cancer cell lines were evaluated. The analogues showed more potent activities than natural acetogenin. One of them, the thiophene-3-carboxamide analogue, strongly inhibited the growth of human lung cancer cell line NCI-H23 in the xenograft mouse assay without critical toxicity.
Assuntos
Acetogeninas/farmacologia , Amidas/farmacologia , Antineoplásicos/farmacologia , Neoplasias Experimentais/tratamento farmacológico , Tiofenos/farmacologia , Acetogeninas/síntese química , Acetogeninas/química , Amidas/administração & dosagem , Amidas/química , Animais , Antineoplásicos/administração & dosagem , Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Estrutura Molecular , Neoplasias Experimentais/patologia , Relação Estrutura-Atividade , Tiofenos/administração & dosagem , Tiofenos/químicaRESUMO
Five novel hybrid molecules of annonaceous acetogenins and insecticides targeting mitochondrial complex I were synthesized and their growth inhibitory activities against 39 human cancer cell lines were investigated. It was revealed that the connecting group between the N-methylpyrazole part and the hydrophobic alkyl chain bearing the THF ring influenced their biological activities significantly. Amide-connected analog 2, in particular, showed selective and very potent activity (<10 nM) against some cancer cell lines.
Assuntos
Acetogeninas/química , Acetogeninas/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Antineoplásicos Fitogênicos/síntese química , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Células HCT116 , Células HT29 , Humanos , Interações Hidrofóbicas e Hidrofílicas , Células MCF-7 , Modelos Químicos , Estrutura Molecular , Relação Estrutura-AtividadeRESUMO
C34-epi and C34-epi-C35-trifluoro analogues of solamin, a mono-THF annonaceous acetogenin, were synthesized. Their inhibitory activity, along with previously synthesized analogues (C35-fluoro, C35-difluoro, and C35-trifluorosolamins), against bovine mitochondrial NADH-ubiquinone oxidoreductase (complex I) was determined. The present study revealed that the methyl group on the γ-lactone moiety is critical to the potent inhibition of complex I by natural acetogenins.
Assuntos
Acetogeninas/química , Acetogeninas/farmacologia , Complexo I de Transporte de Elétrons/antagonistas & inibidores , Lactonas/química , Lactonas/farmacologia , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/enzimologia , Animais , Bovinos , Humanos , Modelos Moleculares , Relação Estrutura-AtividadeRESUMO
A 2 : 1 mixture of NCS and Ph(3)P successfully promoted the anti-dichlorination of olefins to provide corresponding dichlorides, serving as a molecular chlorine surrogate generated in situ.
Assuntos
Alcenos/química , Hidrocarbonetos Clorados/síntese química , Organofosfatos/química , Tiocianatos/química , Hidrocarbonetos Clorados/química , Estrutura MolecularRESUMO
A stereoconvergent access to chiral carbocyclic building blocks is reported. 6-(3'-Hydroxy-4'-methylpent-4'-enyl)-3-methoxy cyclohex-2-enone () that consists of four stereoisomers, i.e., racemic ca. 1 : 1 diastereomers, is converted to enantiomerically pure carbocycles through a combination of regioselective catalytic asymmetric reduction and alkylative remote stereoinduction. The present stereoconvergent strategy has allowed the formal synthesis of bioactive (-)-dysidiolide.
Assuntos
4-Butirolactona/análogos & derivados , Cicloexenos/química , 4-Butirolactona/síntese química , 4-Butirolactona/química , Estrutura Molecular , EstereoisomerismoRESUMO
Palladium-catalyzed cascade C-H alkenylation and arylation provides convenient access to polycyclic aromatic compounds. Treatment of 3-bromoaniline derivatives bearing a bromocinnamyl group on the nitrogen atom with a catalytic amount of [Pd(OAc)(2)] and PCy(3)·HBF(4) in the presence of Cs(2)CO(3) in dioxane affords naphthalene-fused indole derivatives in good yields. This double cyclization reaction is also applicable to heterocyclic substrates, giving fused indoles containing a heteroaromatic ring such as dibenzofuran, dibenzothiophene, carbazole, indole, or benzofuran through heterocyclic C-H arylation. When using a 2,6-unsubstituted aniline derivative, the first C-H arylation preferentially proceeds at the more hindered position of the aniline ring.
Assuntos
Benzofuranos/química , Paládio/química , Compostos Policíclicos/química , Compostos Policíclicos/síntese química , Alquilação , Catálise , Ciclização , Ligação de HidrogênioRESUMO
Allyl N-tosyloxycarbamates are found to be catalytically transformed into ß-brominated oxazolidinones with FeBr(2)/n-Bu(4)NBr in t-BuOH.
Assuntos
Carbamatos/química , Ferro/química , Aminação , Catálise , Halogenação , Estrutura MolecularRESUMO
The alkylation of dienolates generated from 3-methoxycycloalk-2-enones having a 3'-hydroxyl alkenyl chain provides the corresponding quaternized cycloalkenones in a highly diastereoselective manner. The high degree of stereocontrol in the α-quaternization possibly implies intervention of a rigid chelating transition state that allows an efficient 1,4-asymmetric induction to take place.
Assuntos
Técnicas de Química Sintética/métodos , Hidrocarbonetos Alicíclicos/química , Alquilação , Hidrocarbonetos Alicíclicos/síntese química , Estrutura Molecular , EstereoisomerismoRESUMO
The convergent synthesis of C35-fluorinated analogues of solamin, a mono-THF Annonaceous acetogenin, has been achieved by the Sonogashira coupling of the THF ring fragment and the fluorinated γ-lactone fragment. It was revealed that the number of fluorine atoms on the γ-lactone moiety affects the growth inhibitory activities against human cancer cell lines.
Assuntos
Acetogeninas/síntese química , Acetogeninas/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/farmacologia , Desenho de Fármacos , Inibidores do Crescimento/síntese química , Inibidores do Crescimento/farmacologia , Acetogeninas/química , Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Inibidores do Crescimento/química , Halogenação , Humanos , Masculino , Neoplasias/tratamento farmacológicoRESUMO
A novel route to (±)-platencin is reported, in which the highly stereoselective alkylative quaternization of a cyclohexenone scaffold via 1,4-diastereoinduction and two radical carbon-carbon bond-forming reactions that involve titanium(III)-mediated cyclization and stannyl-radical-mediated skeletal rearrangement are utilized.
Assuntos
Aminofenóis/síntese química , Antibacterianos/síntese química , Compostos Policíclicos/síntese química , Aminofenóis/química , Antibacterianos/química , Ciclização , Estrutura Molecular , Compostos Policíclicos/química , EstereoisomerismoRESUMO
The asymmetric alkynylation of aliphatic and aromatic aldehydes with propiolates was mediated by dialkylzinc and a novel prolinol catalyst without high reagent loading and any additives, such as Ti(Oi-Pr)(4), to give the corresponding γ-hydroxy-α,ß-acetylenic esters with high enantiomeric excess of up to 95%.
Assuntos
Aldeídos/química , Alcinos/síntese química , Química Orgânica , Ésteres/síntese química , Propionatos/química , Pirrolidinas/química , Alcinos/química , Catálise/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Compostos Organometálicos/química , Soluções , Estereoisomerismo , Compostos de Zinco/químicaRESUMO
A novel photochemical C-H carbamoylation of an octahydroisoindole derivative with PhNCO has allowed the authors to provide a unique access to a highly functionalized proline motif from which total synthesis of (±)-kainic acid, a bioactive marine alkaloid, has been accomplished.
Assuntos
Alcaloides Indólicos/síntese química , Ácido Caínico/análogos & derivados , Ácido Caínico/síntese química , Alcaloides Indólicos/química , Ácido Caínico/química , Biologia Marinha , Estrutura Molecular , EstereoisomerismoRESUMO
A convergent asymmetric total synthesis of (+)-danicalipin A is accomplished, in which two chlorinated fragments are stereoselectively joined by 1,3-dipolar coupling, leading to the confirmation of the absolute configuration of the natural product.
Assuntos
Lipídeos/síntese química , Lipídeos/química , Estrutura Molecular , Ochromonas/química , EstereoisomerismoRESUMO
The convergent synthesis of fluorescence-labeled solamin, an antitumor Annonaceous acetogenin, was accomplished by two asymmetric alkynylations of 2,5-diformyl tetrahydrofuran with an alkyne tagged with fluorescent groups and another alkyne with an α,ß-unsaturated γ-lactone. Assay for the growth inhibitory activity against human cancer cell lines revealed that the probe with the fluorescent groups at the end of the hydrocarbon chain may have the same mode of action as natural acetogenins. The merged fluorescence of dansyl-labeled solamin and MitoTracker Red suggests that Annonaceous acetogenins localize in the mitochondria.
Assuntos
Acetogeninas/síntese química , Corantes Fluorescentes/síntese química , Acetogeninas/farmacocinética , Antineoplásicos/síntese química , Benzetônio , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Corantes Fluorescentes/farmacocinética , Humanos , Mitocôndrias/metabolismo , Distribuição TecidualRESUMO
The palladium(0)-catalyzed direct construction of bicyclic heterocycles is described. Treatment of propargyl bromides that have nucleophilic functional groups connected by two or three carbon atoms with catalytic [Pd(PPh(3))(4)] affords bis-cyclization products in good yields. The desired bicyclic heterocycles can be obtained selectively when using substrates with appropriate nucleophilic groups. We also describe the reaction of a 2-alkynylazetidine derivative with a catalytic amount of [Pd(PPh(3))(4)] under base-free conditions, which affords the same fused heterocycles as the corresponding propargyl bromides.
Assuntos
Compostos Bicíclicos Heterocíclicos com Pontes/síntese química , Nitrogênio/química , Paládio/química , Pargilina/análogos & derivados , Compostos Bicíclicos Heterocíclicos com Pontes/química , Catálise , Ciclização , Estrutura Molecular , Pargilina/químicaRESUMO
The enantioselective total synthesis of (+)-hexachlorosulfolipid, a cytotoxin found in the Adriatic mussel Mytilus galloprovincialis, is described. The unique chlorinated hydrocarbon motif of the lipid is successfully furnished by a series of dichlorination reactions of chiral epoxides with chlorophosphonium reagent generated in situ from Ph(3)P/NCS. The present total synthesis has allowed the confirmation of the absolute configuration of the natural cytotoxic (+)-hexachlorosulfolipid originally proposed by Fattorusso, Ciminiello, and co-workers.
Assuntos
Lipídeos/síntese química , Mytilus/química , Animais , Simulação por Computador , Lipídeos/química , Lipídeos/isolamento & purificação , Conformação Molecular , EstereoisomerismoRESUMO
A novel palladium(0)-catalyzed domino cyclization of 2-alkynylaziridines with isocyanates through ring expansion is described. Treatment of N-protected 2-(4-aminobut-1-ynyl)aziridine derivatives with a catalytic amount of Pd(PPh(3))(4) and aryl isocyanates in THF at room temperature affords 4-(4,5-dihydropyrrol-2-yl)imidazolidin-2-one derivatives in good yields. Interestingly, bis-adducts were selectively obtained by use of excess isocyanate (5 equiv) at lower reaction temperature.
Assuntos
Aziridinas/química , Aziridinas/síntese química , Compostos Heterocíclicos/química , Isocianatos/química , Compostos Organometálicos/química , Paládio/química , Catálise , Ciclização , Estrutura Molecular , EstereoisomerismoRESUMO
Most Annonaceous acetogenins are characterized by between one and three THF ring(s) with one or two flanking hydroxyl group(s) in the center of a C32/34 fatty acid, and the terminal carboxylic acid is combined with a 2-propanol unit to form an alpha,beta-unsaturated gamma-lactone. While many studies have addressed the properties and synthesis of natural acetogenins due to their attractive biological activities and unique structural features, a number of analogues have also been described. This review covers the design, synthesis, and biological evaluation of acetogenin analogues.
Assuntos
Acetogeninas/síntese química , Acetogeninas/farmacologia , Desenho de Fármacos , Acetogeninas/química , Animais , Química Farmacêutica , Furanos/química , Humanos , Hidrocarbonetos/química , Lactonas/químicaRESUMO
The second-generation approach to (-)-agelastatin A has been established. The present strategy features the FeBr(2)-mediated radical cyclization of 2-cyclopentenyloxycarbonyl azide that allows for the stereoselective installation of a cis-vicinal aminobromo functionality suitable for producing the BCD-ring system of agelastatin A. The aminobromination method streamlines access to oxazolidinone, a key intermediate in the previously reported synthesis, thereby culminating in the new total synthesis of (-)-agelastatin A.