Toll-like receptor-4 activation by subgingival biofilm and periodontal treatment response.

OBJECTIVES: This study aims to investigate longitudinally the activation of Toll-like receptor-4 (TLR-4) by subgingival biofilm samples before and after nonsurgical periodontal therapy (NSPT). MATERIALS AND METHODS: Forty periodontitis patients received NSPT and were reviewed 3 and 6 months post-treatment. Subgingival biofilm was sampled from 4 teeth per patient, at baseline and each follow-up time point. TLR-4 activation was determined using the HEK-BLUE™/hTLR4 system. Changes in TLR-4 activation and probing pocket depths (PPDs) were evaluated using generalised linear models, and the association between TLR-4 activation and pocket reduction (defined as 6-month PPDs ≤ 3mm) was determined using generalised estimating equations. RESULTS: At 6 months, the mean TLR-4 activation by subgingival biofilm samples was significantly reduced from 11.2AU (95%CI 7.1AU, 15.4AU) to 3.6AU (95%CI 2.3AU, 4.8AU, p < 0.001), paralleling significant reductions in mean PPDs at sampled sites. The response to NSPT was associated with longitudinal TLR-4 activation profiles, with significantly higher TLR-4 activation by subgingival biofilm obtained from sites that did not achieve pocket reduction, compared to sites at which pocket reduction was achieved. CONCLUSIONS: The activation of TLR-4 by subgingival biofilm samples was reduced after NSPT, and this reduction was significantly associated with the clinical improvements (PPD reductions) at sampled sites. CLINICAL RELEVANCE: This study demonstrated an association between the longitudinal profile of TLR-4 activation by subgingival biofilm and periodontal treatment response. Longitudinal monitoring of TLR-4 activation by subgingival biofilm may potentially identify non-responsive sites, enabling targeted additional treatment.

Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization.

Gingival and periodontal ligament fibroblasts are functionally distinct cell types within the dento-gingival unit that participate in host immune response. Their microenvironment influences the behavior and immune response to microbial challenge. We developed three-dimensional gingival and periodontal connective tissue equivalents (CTEs) using human fibrin-based matrix. The CTEs were characterized, and the heterogeneity in their innate immune response was investigated. The CTEs demonstrated no to minimal response to planktonic Streptococcus mitis and Streptococcus oralis, while their biofilms elicited a moderate increase in IL-6 and IL-8 production. In contrast, Fusobacterium nucleatum provoked a substantial increase in IL-6 and IL-8 production. Interestingly, the gingival CTEs secreted significantly higher IL-6, while periodontal counterparts produced higher IL-8. In conclusion, the gingival and periodontal CTEs exhibited differential responses to various bacterial challenges. This gives insights into the contribution of tissue topography and fibroblast heterogeneity in rendering protective and specific immune responses toward early biofilm colonizers.

Oral streptococci subvert the host innate immune response through hydrogen peroxide.

In periodontal health, oral streptococci constitute up to 80% of the plaque biofilm. Yet, destructive inflammatory events of the periodontium are rare. This observation suggests that oral streptococci may possess mechanisms to co-exist with the host. However, the mechanisms employed by oral streptococci to modulate the innate immune response have not been well studied. One of the key virulence factors produced by oral streptococci is hydrogen peroxide (H2O2). In mammalian cells, H2O2 triggers the activation of nuclear factor erythroid 2-related factor 2 (Nrf2), a key pathway mediating antioxidant defence. This study aimed to determine (1) if H2O2 producing oral streptococci activated the Nrf2 pathway in macrophages, and (2) if the activation of Nrf2 influenced the innate immune response. We found that oral streptococci downregulated the innate immune response in a H2O2 dependent manner through the activation of the Nrf2. The activation of the Nrf2 signalling pathway led to the inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells (NFĸB), the key transcription factor regulating pro-inflammatory response. This study showed for the first time that oral streptococci are unlikely passive bystanders but could play an active role in the maintenance of periodontal health by preventing overt inflammation.

Neuromodulatory circuit effects on Drosophila feeding behaviour and metabolism.

Animals have evolved to maintain homeostasis in a changing external environment by adapting their internal metabolism and feeding behaviour. Metabolism and behaviour are coordinated by neuromodulation; a number of the implicated neuromodulatory systems are homologous between mammals and the vinegar fly, an important neurogenetic model. We investigated whether silencing fly neuromodulatory networks would elicit coordinated changes in feeding, behavioural activity and metabolism. We employed transgenic lines that allowed us to inhibit broad cellular sets of the dopaminergic, serotonergic, octopaminergic, tyraminergic and neuropeptide F systems. The genetically-manipulated animals were assessed for changes in their overt behavioural responses and metabolism by monitoring eleven parameters: activity; climbing ability; individual feeding; group feeding; food discovery; both fed and starved respiration; fed and starved lipid content; and fed/starved body weight. The results from these 55 experiments indicate that individual neuromodulatory system effects on feeding behaviour, motor activity and metabolism are dissociated.

[Effects of intercropping on soil CO2 and N2O emissions from upland: A review.] / 间作对旱地CO2和N2OæŽ’æ”¾å½±å“çš„ç ”ç©¶è¿›å±•.

Greenhouse gases (GHGs) emission from arable field is a hot topic recently, adopting appropriate cropping systems is an effective way to reduce GHGs emission. This paper reviewed the impacts and mechanisms of intercropping on soil CO2 and N2O emissions in upland field. Rational intercropping systems could increase soil organic carbon (SOC), promote the transformation of straw to SOC, slow down mineralization rate of SOC, and hence reduce soil CO2 emissions. The Poaceae intercropping with legume could maintain the stability of yield while reducing synthetic N inputs, formation of inorganic N by residue decomposition and soil mineral N, and further reducing soil N2O emission. In addition, crop interactions in intercropping system and filed microclimate were important factors on GHGs emission as well. It is necessary to extent the period of researches in field GHGs emission in order to fully understand the underlying mechanisms of GHGs emission in farm land, especially the function of soil microorganisms at molecular level. It would provide theoretical knowledge in building environment-friendly agricultural system in the future.

[Degradation and bioaccumulation characteristics of ciprofloxacin in soil-vegetable system].

A pot experiment was conducted to investigate the effects of applying ciprofloxacin (CIP) on the growth of flowering Chinese cabbage (Brassica campestris), radish (Raphanus sativus), tomato (Solanum lycopersicum) and long bean (Vigna unguiculata), the bioaccumation of CIP in the vegetables, and the degradation of the antibiotics in soil. The results showed that applying 5, 30 and 75 mg x kg(-1) of CIP had no significant effects on the plant biomass of flowering Chinese cabbage, tomato and long bean, but the high concentration of CIP (> or = 30 mg x kg(-1)) inhibited the radish growth significantly. The degradation of CIP in soil was fast at beginning, but slowed down gradually since then. The DT50 and DT90 of CIP in long bean soil were 18.3-43.9 d and 260-738 d, respectively. The CIP could be absorbed by the roots of all the four vegetables and allocated to their aboveground parts, and the CIP residues in the vegetables in creased with the concentration of CIP in soil. The CIP concentration in flowering Chinese cabbage roots was higher than that in the cabbage aboveground parts, that in radish showed a higher concentration in aboveground parts than in roots, and the CIP concentration in tomato and long bean was in the same order of root > stem > leaf.