Effects of a 10-d Military Field Exercise on Body Composition, Physical Performance, and Muscle Cells in Men and Women.

PURPOSE: This study aimed to investigate the effects of a demanding military field exercise on physical performance, body composition, and muscle cellular outcomes in men and women. METHODS: Ten men (20.5 ± 0.5 yr) and 8 women (21.4 ± 1.4 yr) completed a 10-d field exercise consisting of extensive physical activity with food and sleep restriction. Acquisition of body composition, physical performance, blood, and muscle biopsies samples were done before and 1, 7, and 14 d after the exercise. RESULTS: There were no sex differences in the response to the exercise. Body mass was decreased with 5.6% ± 1.8% and fat mass with 31% ± 11% during the exercise. Both were still reduced after 14 d (2.5% ± 2.3%, P < 0.001, and 12.5% ± 7.7%, P < 0.001, respectively). Isometric leg strength did not change. Peak leg extension torque at 240°·s -1 and counter movement jump height were reduced with 4.6% ± 4.8% ( P = 0.012) and 6.7% ± 6.2% ( P < 0.001), respectively, and was still reduced after 14 d (4.3% ± 4.2%, P = 0.002, and 4.1% ± 4.7%, P = 0.030). No changes occurred in fiber CSA, fiber types, proteins involved in calcium handling, or HSP70. During the exercise, αB-crystallin levels decreased by 14% ± 19% ( P = 0.024) in the cytosolic fraction and staining intensity on muscle sections tended to increase (17% ± 25%, P = 0.076). MuRF1 levels in the cytosolic fraction tended to decrease (19% ± 35%) and increased with 85% ± 105% ( P = 0.003) in the cytoskeletal fraction 1 wk after the exercise. CONCLUSIONS: The field exercise resulted in reduced body mass and physical performance in both sexes. The ability to produce force at high contraction velocities and explosive strength was more affected than isometric strength, but this was not related to any changes in fiber type composition, fiber area, Ca 2+ handling, or fiber type-specific muscle damage.

Sex differences in the physiological response to a demanding military field exercise.

PURPOSE: To investigate sex differences in the effect of a military field exercise on physical performance, body composition, and blood biomarkers. METHODS: Measurements were done in 23 male and 12 female conscripts before, and 0, 1, 3, 7, and 14 days after a 6-day military field exercise. RESULTS: During the field exercise, body mass decreased more in men (-6.5 ± 1.1 kg) than in women (-2.7 ± 0.7 kg), and muscle mass decreased only in men (-2.7 ± 1.0 kg). Body composition recovered within one week. Performance decreased, with no differences between men and women for countermovement jump (CMJ,-19 ± 8 vs. -18 ± 11%), medicine ball throw (MBT, -11 ± 7 vs. -11 ± 7%), and an anaerobic performance test (EVAC, -55 ± 22 vs. -47 ± 31%, men and women, respectively). MBT and EVAC performance recovered within two weeks, whereas CMJ performance was still reduced in men (-17 ± 6%) and women (-9 ± 8%) after two weeks recovery, with a larger reduction in men. Both men and women decreased [IGF-1] (-28 ± 9 vs. -41 ± 8%) and increased [cortisol] (26 ± 26 vs. 66 ± 93%, men and women, respectively) during the exercise. Most biomarkers returned to baseline values within one week. CONCLUSIONS: Men lost more body mass and muscle mass than women during a field exercise, but these differences did not lead to sex differences in changes in explosive strength and anaerobic performance. However, women recovered explosive strength in the legs faster than men.

The use of organotypic hippocampal slice cultures to evaluate protection by non-competitive NMDA receptor antagonists against excitotoxicity.

There is great interest in testing neuroprotectants which inhibit the neurodegeneration that results from excessive activation of N-methyl-D-aspartate (NMDA) receptors. As an alternative to in vivo testing in animal models, we demonstrate here the use of a complex in vitro model to compare the efficacy and toxicity of NMDA receptor inhibitors. Organotypic hippocampal slice cultures were used to compare the effectiveness of the Alzheimer's disease drug, memantine, the Parkinson's disease drug, procyclidine, and the novel neuroprotectant, gacyclidine (GK11), against NMDA-induced toxicity. All three drugs are non-competitive NMDA receptor open-channel blockers that inhibit excitotoxic injury, and their neuroprotective capacities have been extensively investigated in vivo in animal models. They have also been evaluated as potential countermeasure agents against organophosphate poisoning. Quantitative densitometric image analysis of propidium iodide uptake in hippocampal regions CA1, CA3 and DG, showed that, after exposure to 10microM NMDA for 24 hours, GK11 was the most potent of the three drugs, with an IC50 of about 50nM and complete protection at 250nM. When applied at high doses, GK11 was still the more potent neuroprotectant, and also the least cytotoxic. These findings are consistent with those from in vivo tests in rodents. We conclude that the slice culture model provides valuable pre-clinical data, and that applying the model to the screening of neuroprotectants might significantly limit the use of in vivo tests in animals.

Measurements with fluorescent probes in primary neural cultures; improved multiwell techniques.

INTRODUCTION: Fluorescence imaging techniques are valuable tools for the pharmacological characterization of CNS drugs. Dissected cerebellar granule neurons (CGN) are an important model system in the study of mechanisms of excitotoxicity, glutamate receptors and transporters. Widely applied techniques use fluorescent probes loaded in neural cells cultured on glass supports. CGN, however, require at least 7 days for differentiation and over time cells tend to cluster and loose adherence to the glass substrate. This problem is accentuated in small wells (e.g. 96-well plates). METHODS: CGN were grown on large coverslips (60 x 24 mm) and measurements made with a designed mountable multiwell in 48 regions on 4 coverslips at a time. The UV ratiometric probe fura-2 was used to measure glutamatergic calcium ([Ca(2+)](i)) responses induced by NMDA. The IC(50) of NMDA receptor antagonists was determined from inhibition curves with 6 doses and 8 parallels per experiment. RESULTS: The method was validated by comparing with published data for the dose response to NMDA and glycine and IC(50) values for ion-channel block by Mg(2+) and MK-801. DISCUSSION: Resolution is enhanced with the new technique since it allows measurement of multiple doses on cells from the same batch. It has advantages to cuvette techniques because cells have intact dendritic tree and synaptic function and it is a convenient method to obtain reliable dose-response curves for NMDA channel modulators on differentiated neural cells.

Pre-junctional effects of oximes on [3H]-acetylcholine release in rat hippocampal slices during soman intoxication.

In this study, the non-reactivating effects of oximes in the hippocampus of the rat are investigated. The potassium (51 mM) evoked release of [(3)H]-acetylcholine and the liberation of [(3)H]-choline were determined in hippocampal slices following in vitro exposure to soman and five oximes (toxogonin, HI-6, HLö-7, P2S and 2-PAM) in separate experiments by superfusion. In the absence of soman, toxogonin and HLö-7 in particular induced a concentration dependent significant increase in the evoked release of [(3)H]-acetylcholine. There was also a significant effect of HI-6, but the effect was much smaller. Two pralidoxime salts, P2S (methanesulfonate salt) and 2-PAM (methiodide salt), had similar but lower effects that were only observed at relatively high concentrations. Experiments performed following complete inhibition of the acetylcholinesterase activity by soman (1.0 microM) showed that HI-6 and HLö-7 induced a significant decrease in the potassium-evoked release of [(3)H]-acetylcholine, while the liberation of [(3)H]-choline increased. Toxogonin, P2S and 2-PAM did not reduce significantly the evoked release of [(3)H]-acetylcholine. Only limited reactivation of the acetylcholinesterase activity was observed in superfusion experiments with toxogonin, HI-6, P2S and 2-PAM following exposure of hippocampal slices to soman. However, HLö-7 was proved to be relatively more effective in reactivating the acetylcholinesterase activity at high concentrations (50 and 200 microM). The acetylcholinesterase activity was reactivated to approximately 12% and 40% of control, respectively. It is concluded that HI-6 and HLö-7 have important non-acetylcholinesterase reactivating properties following soman poisoning, as may be seen by the significant reduction in the evoked release of [(3)H]-acetylcholine effected by these oximes. HLö-7 is of particular interest in view of its ability to additionally improve reactivation of the acetylcholinesterase activity.