[Identification of a Facultative Bacterium Strain with the Ability to Methylate Mercury Under Both Aerobic and Anaerobic Conditions].

A strain with the ability to methylate mercury under both the aerobic and anaerobic conditions was isolated from soil of the water-level-fluctuation-zone in the Three Gorge Reservoir in Shibaozhai Village, Zhongxian Country, Chongqing, China (E108°12'3″ and N30°24'36″). The soil was classified as Purple soil with a pH of 7.97 (0-20 cm depth). The isolation was performed under 1.0 mg·L-1 HgCl2 conditions. After its morphological and physiological characterization, and its phylogenetic analysis using 16S rDNA gene sequence, the strain was identified as Pseudomonas fluorescens sp., and named as Pseudomonas fluorescens XD-MeHg-B2 (GenBank accession number: KU954349). On one hand, at 30℃ under aerobic condition, the concentration of methylmercury (MeHg) in the PBS (phosphate buffer saline) solution, which was inoculated with 1×1011 cfu·mL-1 suspension of P. fluorescens XD-MeHg-B2 and an initial Hg2+ of 200 ng·L-1, was exponentially increased to 1.22 ng·L-1±0.15 ng·L-1 after 60 min incubation and then approached to the maximum of 3.85 ng·L-1±0.33 ng·L-1 160 min after incubation. The largest mercury methylation rate was 1.93%. On the other hand, at 30℃ under anaerobic condition, the concentration of MeHg in the PBS solution, which was also inoculated with 1×1011 cfu·mL-1 suspension of P. fluorescens XD-MeHg-B2 and an initial Hg2+ of 200 ng·L-1, was 2.86 ng·L-1±0.73 ng·L-1 and the largest mercury methylation rate was 1.43% 180 min after incubation. As a result, P. fluorescens XD-MeHg-B2 showed its ability to methylate mercury under both aerobic and anaerobic conditions while with a comparatively hysteretic and lower ability of mercury methylation. These results demonstrated that P. fluorescens XD-MeHg-B2 could be a promising candidate for further studies on mercury biogeochemical cycle, particularly under dry-wet alternative conditions.

[Transgenic mouse models of the truncated platelet integrin ß3 cytoplasmic tail established by stem cell transplantation].

This study was purpose to establish the transgenic mouse models of the truncated platelet integrin ß3 by retrovirus-infected hematopoietic stem cells (HSCs) transplantation and to provide the basis for further study of the role of integrin ß3 cytoplasmic domain in platelet bi-directional signaling pathways. Wild-type ß3, ß3-Δ759 (R(760) GT(762) truncated ß3) and ß3-Δ754 (T(755) NITYRGT(762) truncated ß3) cDNAs were subcloned into MSCV MigR1 retroviral vector bearing a GFP gene and packaged into infective retrovirus with BOSC23 cell strain. The bone marrow HSCs of the ß3 deficient mice were infected by the retroviruses, and transplanted into lethally-irradiated wild type C57BL/6 mice. GFP positive rate and surface ß3 expression of the recipients' platelets at 6 to 8 weeks after transplantation were detected by flow cytometry to evaluate the transgenic efficiency. The results showed that four kinds of transgenic mouse models including vector, wild-type ß3, ß3-Δ759 and ß3-Δ754 were established successfully. GFP positive rates of transgenic mouse platelets ranged from 18% to 66% and the ß3 expression of transgenic mouse reached heterozygote (ß3(+/-) level of mouse). It is concluded that establishment of transgenic mouse models mediated by retrovirus-infected HSCs transplantation is a feasible, fast, and high throughput transgenic approach and laid a solid foundation for further research on the role of integrin ß3 cytoplasmic domain for bi-directional signaling of platelets in vivo, and for the gene therapy of platelet disorders.