RESUMO
Our study showed a statistically significant incidence of pulmonary edema in mice receiving amiodarone and 100% oxygen. This finding, together with a variety of clinical reports, indicates that in patients receiving amiodarone therapy, FiO2 should be maintained at the lowest possible level, consistent with adequate oxygenation.
Assuntos
Amiodarona/efeitos adversos , Antiarrítmicos/efeitos adversos , Pulmão/metabolismo , Oxigênio/metabolismo , Edema Pulmonar/induzido quimicamente , Vasodilatadores/efeitos adversos , Animais , Masculino , Camundongos , Camundongos Endogâmicos , Edema Pulmonar/metabolismo , RespiraçãoAssuntos
Acetazolamida/uso terapêutico , Anticonvulsivantes/uso terapêutico , Epilepsia/tratamento farmacológico , Epilepsia/prevenção & controle , Acetazolamida/administração & dosagem , Adolescente , Adulto , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/uso terapêutico , Anticonvulsivantes/administração & dosagem , Anti-Hipertensivos/administração & dosagem , Anti-Hipertensivos/uso terapêutico , Aspirina/administração & dosagem , Aspirina/uso terapêutico , Encéfalo/fisiopatologia , Quimioterapia Combinada , Epilepsia/fisiopatologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nimodipina/administração & dosagem , Nimodipina/uso terapêuticoRESUMO
Outer-membrane protein (OMP)-enriched preparations from avian (turkey/TT3 and parrot/VS1) and mammalian (sheep abortion/B577) strains of Chlamydia psittaci were compared by immunoblotting using sera from turkeys exposed to these strains. Turkeys inoculated with avian chlamydiae became infected and developed strong serological responses, but turkeys inoculated with B577 failed to develop detectable serological responses. Sera from turkeys exposed to either of the two avian strains could be differentiated on the basis of immunoreaction patterns with OMPs of homologous and heterologous strains. Fewer bands and often weaker reactions were detected using sera from TT3- and VS1-inoculated birds with the heterologous avian strain. Sera from turkeys inoculated with either avian strain reacted with the 97,400-molecular weight (MW) protein. The sera reacted with the major outer-membrane protein (MOMP) of the homologous strains but not consistently with the MOMP of the heterologous strain. Results suggest that the 97,400-MW protein is highly immunogenic for turkeys and antigenically more complex than the MOMP.
Assuntos
Anticorpos Antibacterianos/sangue , Proteínas da Membrana Bacteriana Externa/imunologia , Chlamydophila psittaci/imunologia , Doenças das Aves Domésticas/imunologia , Psitacose/veterinária , Perus/imunologia , Animais , Immunoblotting/veterinária , Psitacose/imunologia , Especificidade da Espécie , Perus/microbiologiaRESUMO
Male Holtzman rats were given subcutaneous doses of a purified Pasteurella multocida group D heat-labile toxin on alternate days for up to 22 days. Rats were necropsied at 18 days or 36 days (14 days after last dose of toxin) or when moribund, and testicles were taken for histologic and ultrastructural examination. Other selected tissues, including liver and spleen, were taken for histologic examination. Histologically, testicular and splenic lesions occurred more consistently and at much smaller doses when compared with lesions in other target organs such as liver. Testicular and splenic lesions were present in all rats (6/6) given 0.8 micrograms/kg toxin and were seen in some rats (1/6) given as little as 0.2 micrograms/kg toxin. Only 3/6 rats given 0.8 micrograms/kg toxin had hepatic lesions; no hepatic lesions were seen at doses of 0.2 micrograms/kg. Testicles from toxin-treated rats were smaller and weighed less than controls. Seminiferous tubules were moderately dilated and lined by polygonal sertoli cells. The normal spermatogenic maturation sequence and mature spermatids were absent, and many tubules contained multinucleate spermatocytes. Severely affected tubules were necrotic and mineralized. Ultrastructurally, there was necrosis of adluminal spermatocytes, multinucleate cell formation, and spaces between Sertoli cell plasma membranes. Testicular lesions were similar to those described for vitamin D-deficient rats, vitamin A-deficient rats, vasectomized rats, and rats given intravenous tumor necrosis factor; however, rats given lethal doses of toxin did not have elevated levels of TNF alpha activity.
Assuntos
Proteínas de Bactérias , Toxinas Bacterianas/toxicidade , Pasteurella multocida , Túbulos Seminíferos/patologia , Animais , Histocitoquímica , Masculino , Microscopia Eletrônica , Tamanho do Órgão/efeitos dos fármacos , Ratos , Túbulos Seminíferos/efeitos dos fármacos , Túbulos Seminíferos/ultraestrutura , Células de Sertoli/efeitos dos fármacos , Espermatogênese/efeitos dos fármacos , Baço/patologia , Testículo/patologia , Testículo/ultraestrutura , Fator de Necrose Tumoral alfa/toxicidadeRESUMO
Plasma and joint fluids from turkeys experimentally inoculated with Chlamydia psittaci strain TT3 were evaluated by immunoblotting to identify antibodies elicited by chlamydial antigens during the course of infection. Protein antigens from elementary bodies of TT3 were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred electrophoretically to nitrocellulose before being probed with plasma or synovial fluid from TT3-inoculated birds. The major outer-membrane protein (MOMP), the 60,000-molecular-weight proteins, and a 97,400-molecular-weight protein were the predominant antigens recognized by IgG in the plasma and joint fluids. Plasma IgG specific for the 97,400 protein band was first detectable at day 10 postinoculation (PI). Antibodies to the 60,000-molecular-weight protein and MOMP were first detected at days 14-17 PI and at days 7-10 PI, respectively, in some birds, and as late as days 36-42 PI and days 42-70 PI in others. The antibodies were still present at day 142 PI. Immunoblotting techniques indicated that the antigens to which these antibodies were reacting were protein. These observations may have implications for the development of serodiagnostic assays as well as the identification of potential proteins for subunit immunogens in birds.
Assuntos
Anticorpos Antibacterianos/biossíntese , Chlamydophila psittaci/imunologia , Doenças das Aves Domésticas/imunologia , Psitacose/veterinária , Perus , Animais , Proteínas da Membrana Bacteriana Externa/efeitos dos fármacos , Proteínas da Membrana Bacteriana Externa/imunologia , Reações Cruzadas , Eletroforese em Gel de Poliacrilamida , Endopeptidases/farmacologia , Epitopos/análise , Immunoblotting , Imunoglobulina G/imunologia , Ácido Periódico/farmacologia , Psitacose/imunologia , Líquido Sinovial/imunologia , Tripsina/farmacologiaRESUMO
Chlamydia psittaci is a diverse group of organisms that affects birds and mammals. The number of biovars is unknown, and less is known about the number of serovars. Our restriction endonuclease analysis indicates that there are at least 5 biovars including avian, abortion-enteritis, IPA, M56, and GPIC. Monoclonal antibody studies revealed 4 serovars in the avian biovar. Monoclonal antibody studies have not yet been performed to identify multiple serovars in the other biovars; however, microimmunoassay studies indicate that a number of serovars may exist in the abortion and arthritis biovars. Of the 4 avian serovars, 2 are of major importance in the US avian population. These 2 serovars, psittacine and turkey, are each associated with important host preferences and disease characteristics. The turkey isolates have all been associated with either a serious disease in birds or human beings or with major epizootics in turkeys, often resulting in human disease. The psittacine serovar has been associated with serious disease in human beings; however, human involvement is usually limited to sporadic cases following exposure to companion birds or pigeons. The other 2 serovars, German duck and WC, are single isolates and their distributions and disease characteristics are not known.
Assuntos
Doenças das Aves/microbiologia , Chlamydophila psittaci/classificação , Psitacose/veterinária , Animais , Aves , Chlamydophila psittaci/genética , Chlamydophila psittaci/imunologia , Psitacose/microbiologiaRESUMO
Three groups of turkeys were inoculated with strains of C. psittaci (B577, VS1, TT3) from different restriction endonuclease groups. Turkeys were necropsied at 15 times through post-inoculation day 70. Birds infected with the TT3 strain were lethargic and had decreased body weight. After forced exercise, dyspnea was seen in VS1-infected turkeys. Pericarditis was the most severe lesion in TT3-infected birds. Airsacculitis and bronchopneumonia were the most severe lesions in VS1-infected turkeys. Lateral nasal adenitis was in both VS1- and TT3-infected birds. Only mild peribronchial pneumonia was in B577-infected turkeys. Chlamydial antigen, identified by light microscopy using an immunoperoxidase technique, was seen from post-inoculation days 9 through 50 in the lateral nasal gland and at earlier times in other tissue from VS1- and TT3-infected turkeys. No chlamydial antigen was detected in tissue from B577-infected birds. These studies showed that chlamydial strains from different restriction endonuclease groups are associated with distinct disease syndromes in turkeys.
Assuntos
Pericárdio/patologia , Doenças das Aves Domésticas/patologia , Psitacose/veterinária , Sistema Respiratório/patologia , Perus , Sacos Aéreos/patologia , Animais , Peso Corporal , Técnicas Imunoenzimáticas , Pulmão/patologia , Mucosa Nasal/patologia , Psitacose/patologia , Traqueia/patologiaRESUMO
Multiple outbreaks of acute severe fibrinopurulent lesions of the eyelids occurred in chickens and turkeys. Lesions began as tiny foci of epidermal necrosis and ulceration and spread to involve the entire eyelid. Scabs overlying the epidermis contained large gram-positive cocci; lesser numbers of small cocci and gram-negative bacilli were in more superficial areas. Staphylococcus hyicus was isolated from birds in all stages of the disease. Escherichia coli and Streptococcus sp. were isolated only during severe stages; no anaerobic bacterial pathogens were isolated. Vasculitis and perivascular lymphocytic infiltrates in deep layers of the dermis suggested that a staphylococcal toxin may have been involved. The disease was not reproduced by scarifying S. hyicus onto the eyelids or by intravenous inoculation of retrovirus-infected chickens.
Assuntos
Blefarite/veterinária , Galinhas , Conjuntivite Bacteriana/veterinária , Doenças Palpebrais/veterinária , Doenças das Aves Domésticas/patologia , Perus , Animais , Blefarite/patologia , Conjuntivite Bacteriana/patologia , Infecções por Escherichia coli/patologia , Infecções por Escherichia coli/veterinária , Pálpebras/patologia , Feminino , Aparelho Lacrimal/patologia , Masculino , Necrose , Infecções Cutâneas Estafilocócicas/patologia , Infecções Cutâneas Estafilocócicas/veterinária , Staphylococcus/isolamento & purificação , Infecções Estreptocócicas/patologia , Infecções Estreptocócicas/veterináriaRESUMO
From January 1982 through December 1985, 11 newborn artiodactyls died with clinical and/or pathologic evidence of cardiomyopathy. Clinical signs were inability to rise, depression, failure to nurse, hypothermia, and shivering. Macroscopically, the animals had mild to marked dilatation and thinning of the interventricular septum and left ventricular free wall. Histologic findings included thinning and waviness of myofibers and acute myodegeneration and myocytolysis.
Assuntos
Animais Recém-Nascidos , Artiodáctilos , Cardiomiopatia Dilatada/veterinária , Miocárdio/patologia , Animais , Cardiomiopatia Dilatada/patologia , Feminino , Ventrículos do Coração/patologia , MasculinoRESUMO
Atherosclerosis was diagnosed on necropsy in 21 dogs in a 14-year period. Nine dogs died and 12 were euthanatized because of complications associated with the disease. The mean age was 8.5 +/- 0.5 years; 18 dogs were male. Three breeds (Miniature Schnauzer, Doberman Pinscher, and Labrador Retriever) had a higher prevalence of the disease than other breeds in the canine necropsy population of The Animal Medical Center. Most common clinical signs were lethargy, anorexia, weakness, dyspnea, collapse, and vomiting. Hypercholesterolemia, lipidemia, and hypothyroidism were common in affected dogs tested, and protein electrophoresis revealed high values for alpha 2 and beta fractions in all dogs tested. Electrocardiography indicated conduction abnormalities and myocardial infarction in 3 of 7 dogs. Necropsy revealed that affected arteries (including coronary, myocardial, renal, carotid, thyroidal, intestinal, pancreatic, splenic, gastric, prostatic, cerebral, and mesenteric) were yellow-white, thick and nodular, and had narrow lumens. Myocardial fibrosis and infarction also were observed in the myocardium. Histologically, affected arterial walls contained foamy cells or vacuoles, cystic spaces, mineralized material, debris with or without eroded intima, and degenerated muscle cells.
Assuntos
Arteriosclerose/veterinária , Doenças do Cão/diagnóstico , Animais , Arteriosclerose/patologia , Arteriosclerose/fisiopatologia , Doenças do Cão/patologia , Doenças do Cão/fisiopatologia , Cães , Eletrocardiografia , Feminino , MasculinoRESUMO
Cardiomyopathy associated with vitamin E deficiency was diagnosed in more than 100 ruminants and primates and in 106 embryos and newly hatched chicks and ducklings. Affected bird embryos failed to pip the eggshell and died inside. Newly hatched chicks and ducklings and neonatal ruminants were weak, had difficulty standing or rising, and died within a few days. Death usually occurred without premonitory signs of disease in juvenile and adult animals. On gross examination, the hearts of the neonatal ruminants had areas of mottled, pinkish-tan myocardium. The hearts of the embryos and newly hatched birds were edematous, pinkish, and pale. In the juvenile ruminants, irregular, whitish patches or pale areas were seen in the myocardium. Histologically, there was multifocal myocytolysis in the myocardium of the neonatal and juvenile ruminants and embryos and newly hatched birds. Focal disseminated or diffuse myocardial fibrosis and myocytolysis were observed in the hearts of the adult animals. Plasma alpha tocopherol values were low enough in all species to be considered deficient. These values increased significantly after the addition of alpha tocopherol and/or vitamin E to the diets of the animals. Cardiomyopathy has not been diagnosed in any of the same groups of animals since supplementation was initiated.
Assuntos
Animais de Zoológico , Doenças das Aves/patologia , Cardiomiopatias/veterinária , Doenças dos Macacos/patologia , Ruminantes , Deficiência de Vitamina E/veterinária , Animais , Doenças das Aves/tratamento farmacológico , Aves , Cardiomiopatias/tratamento farmacológico , Cardiomiopatias/etiologia , Cardiomiopatias/patologia , Feminino , Masculino , Doenças dos Macacos/tratamento farmacológico , Miocárdio/patologia , Selênio/uso terapêutico , Vitamina E/uso terapêutico , Deficiência de Vitamina E/complicações , Deficiência de Vitamina E/tratamento farmacológico , Deficiência de Vitamina E/patologiaRESUMO
Between November 1979 and July 1982, 7 captive gelada baboons (Theropithecus gelada) died; 5 of them died unexpectedly, 1 died after a 4-month history of heart failure, and 1 was anemic and dyspneic for 2 days before death. Of those that died unexpectedly, 1 was anemic and 4 were clinically normal. At necropsy, all baboons had white or pale patches of myocardium. Histologically, fibrosis and acute myocytolysis were observed in the myocardium. Three affected baboons were tested for plasma alpha-tocopherol content and were found deficient. Four unaffected baboons were given vitamin E for 24 months, and plasma alpha-tocopherol content returned to normal. Blood selenium content was determined in 1 affected baboon and was normal.
Assuntos
Cardiomiopatias/veterinária , Cercopithecidae , Doenças dos Macacos/etiologia , Theropithecus , Deficiência de Vitamina E/veterinária , Anemia/etiologia , Anemia/veterinária , Animais , Animais de Zoológico , Cardiomiopatias/etiologia , Criança , Feminino , Humanos , Masculino , Doenças dos Macacos/diagnóstico , Deficiência de Vitamina E/complicaçõesRESUMO
Listeriosis was diagnosed in 7 bushy-tailed jirds (Sekeetamys calurus). Death in all cases was acute, and there were no clinical signs. Necropsy revealed necrotizing lesions in the liver, intestines, spleen, and hepatic and mesenteric lymph nodes. On histologic examination large numbers of gram-positive coccobacilli were seen in the lesions. Pneumonitis was seen in 6 jirds, and small numbers of bacteria were seen in the lungs in all cases except one. Diagnosis was based on bacterial culture of the liver in 2 jirds and on immunofluorescence of bacteria when tissue specimens from 2 other jirds were stained with fluorescein-labelled antiglobulins specific for Listeria monocytogenes.
Assuntos
Gerbillinae , Listeriose/veterinária , Doenças dos Roedores/patologia , Animais , Animais de Zoológico , Intestinos/patologia , Listeriose/patologia , Fígado/patologia , Pulmão/patologia , Linfonodos/patologia , Necrose , New York , Fibrose Pulmonar/patologia , Fibrose Pulmonar/veterinária , Baço/patologiaAssuntos
Animais de Zoológico , Aspergilose/veterinária , Dermatomicoses/veterinária , Lagartos , Pneumopatias Fúngicas/veterinária , Animais , Animais de Zoológico/microbiologia , Aspergilose/microbiologia , Aspergilose/patologia , Aspergillus/isolamento & purificação , Dermatomicoses/microbiologia , Dermatomicoses/patologia , Lagartos/microbiologia , Pneumopatias Fúngicas/microbiologia , Pneumopatias Fúngicas/patologiaRESUMO
Degenerative myelopathy was diagnosed in six Mongolian wild horses. Three of the horses had a history of ataxia dating from birth to 3 months of age. The clinical signs were uncoordinated movement of the hindlimbs and an abnormally wide-based gait and stance. The other 3 horses had mild ataxia. There were no gross lesions in the brain, vertebrae, or spinal cord. Histologic examination revealed degeneration of the neural processes in the ventral and lateral funiculi of all 6 horses. Myelin sheaths were dilated and vacuolated, and there were swollen, fragmented, or lysed axons. Neuronal degeneration, phagocytosis, and accumulation of periodic acid-Schiff-positive, xylol-insoluble lipopigment were observed in the affected neurons of the dorsal root ganglia. The plasma alpha-tocopherol values of 5 of the affected horses ranged from less than 0.03 to 0.08 (mean, 0.04 +/- 0.01) mg/dl. Seven clinically normal horses from the same herd had a range of less than 0.03 to 0.3 (mean, 0.11 +/- 0.02) mg/dl, which was low enough to be considered deficient.