Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia.

Specific growth rate (G(S) ) and white skeletal muscle composition were measured in the mummichog Fundulus heteroclitus over a period of 28 days at four levels of dissolved oxygen (DO): severe hypoxia (c. 1.2 mg O(2) l(-1) ), moderate hypoxia (3.0 mg O(2) l(-1) ), normoxia (7.1 mg O(2) l(-1) ) and hyperoxia (10.6 mg O(2) l(-1) ). The G(S) was calculated over 0-8, 0-14, 0-28 and 14-28 days, and muscle protein, lactate dehydrogenase (LDH), DNA, RNA and water were measured at 0, 8, 14 and 28 days. Exposure of fish to severe hypoxia was associated with significantly reduced G(S) , lower muscle protein content and lower RNA:DNA compared with other DO treatments. When calculated over the first and second half of the 28 day exposure, however, G(S) of fish in severe hypoxia increased significantly during the second two-week interval, to the same rate as that of normoxic fish. Muscle LDH activity and water content were not significantly affected by DO level. Neither moderate hypoxia nor hyperoxia significantly affected G(S) or any biochemical variable. The results demonstrate that F. heteroclitus can tolerate wide variation in ambient oxygen concentration and, during prolonged exposure to severe hypoxia, shows significant compensation for the initial negative effects on growth. The capacity of F. heteroclitus to grow over a wide range of DO probably contributes to its ability to exploit habitats characterized by marked variation in oxygen availability.

Development, validation and field application of an RNA-based growth index in juvenile plaice Pleuronectes platessa.

A general mechanism relating RNA concentration and growth rate is derived from four physiological assumptions and developed into a growth index for juvenile plaice Pleuronectes platessa. The index describing instantaneous growth rates (G, day⁻¹) in the laboratory with the lowest Akaike information criterion with small-sample bias adjustment was a function of RNA concentration (R, g(RNA)g⁻¹(wet mass)), temperature (T, ° K), body mass (M, g) and DNA concentration (D, g(DNA)g⁻¹(wet mass)): G = ß0 + ß(R) R + ß(T)T + ß(T2)T² + ß(M)M + ß(D)D + ß(RT)RT. RNA concentration began to respond to changes in feeding conditions within 8 days, suggesting that the index reflects growth rate in the short-term. Furthermore, the index distinguished between rapid growth and negative growth of juvenile P. platessa measured directly in laboratory and field enclosures, respectively. An application of the RNA-based growth index at two beaches on the west coast of Scotland suggested that the growth of juvenile P. platessa varies considerably in space and time and is submaximum in late summer.

Characterizing the escape response of juvenile summer flounder Paralichthys dentatus to diel-cycling hypoxia.

Swimming speed, angular correlation and expected displacement were measured in juvenile summer flounder Paralichthys dentatus acclimated to either oxygen saturation (c. 7.8 mg O(2) l(-1); saturation-acclimated fish) or diel-cycling hypoxia (cycling between 11.0 and 2.0 mg O(2) l(-1)) for 10 days and subsequently exposed to more severe diel-cycling hypoxia (cycling between 7.0 and 0.4 mg O(2) l(-1)). Saturation-acclimated P. dentatus exhibited an active response to declining dissolved oxygen (DO) by increasing swimming speed, angular correlation and expected displacement to peak levels at 1.4 mg O(2) l(-1) that were 3.5, 5.5 and 4.2 fold, respectively, greater than those at DO saturation. Diel-cycling hypoxia-acclimated P. dentatus also exhibited an active response to declining DO, although it was relatively less pronounced. Diel-cycling hypoxia-acclimated P. dentatus swimming speed, however, still doubled as DO decreased from 7.0 to 2.8 mg O(2) l(-1). Diel-cycling hypoxia-acclimated P. dentatus did not recover as well from low DO exposure as did saturation-acclimated fish. This was reflected in their relatively more random swimming (low angular correlation between successive moves) and poor maintenance of rank order between individuals during the recovery phase. Even saturation-acclimated P. dentatus did not resume swimming at speeds observed at saturation until DO was 4.2 mg O(2) l(-1). Paralichthys dentatus were very sensitive to decreasing DO, even at DO levels that were not lethal or growth limiting. This sensitivity and their poor recovery may preclude juvenile P. dentatus from using highly productive nursery habitats affected by diel-cycling hypoxia.