Effect of Bacillus toyonensis BCT-7112T supplementation on growth performance, intestinal morphology, immune-related gene expression, and gut microbiome in Barbary ducks.

This study aimed to investigate the effect of Bacillus toyonensis BCT-7112T supplementation on growth performance, intestinal morphology, immune-related gene expression, and the cecal microbiota of meat ducks. A total of 150 one-day-old male Barbary ducks were divided into 3 groups with 5 replicates (n = 10 ducks per replicate) by completely randomized design and offered diets supplemented with the commercial product Toyocerin (containing 1 × 109B. toyonensis BCT-7112T viable spores/g product) at the levels of 0, 500, or 1,000 mg/kg (0, 500, or 1,000 ppm), respectively, for 8 wk. The results showed that although ducks in the 500 ppm B. toyonensis BCT-7112T group displayed numerically better values (e.g., weight gain and feed conversion ratio) than those in the control group, the growth performance of ducks fed diets supplemented with B. toyonensis BCT-7112T did not differ significantly from that of the control group (P > 0.05). There were no significant differences in the intestinal mucosal morphology of ducks across the experimental groups (P > 0.05). However, ducks in the 500 ppm B. toyonensis BCT-7112T group showed a trend of greater values, for example, villus height per crypt depth of duodenum (P = 0.16) and ileum (P = 0.12) compared with those in the control group. The relative expression of immune-related genes, for example, interferon (IFN) and interleukin-6 (IL-6) in the meat duck spleen was significantly lower in both B. toyonensis BCT-7112T groups at 14 d and 35 d than in the control group (P < 0.05). Beta diversity analysis of the cecal microbiota of ducks in either the 500 ppm or the 1,000 ppm B. toyonensis BCT-7112T group showed to have higher diversity than that in the control group, where at the phylum level, Bacteroidetes was the most abundant, followed by Firmicutes, and at the genus level, Bacteroides, Fusobacterium, and Ruminococcaceae were the top 3 most abundant genera. In conclusion, our study demonstrates that 500 ppm supplementation with B. toyonensis BCT-7112T in duck diets can reduce proinflammatory cytokine gene expression, improve immunological function, and increase the variety of microbial communities in the ceca of meat-type ducks.

Alterations in skeletal muscle abundance of protein turnover, stress, and antioxidant proteins during the periparturient period in dairy cows fed ethyl-cellulose rumen-protected methionine.

Skeletal muscle turnover helps support the physiological needs of dairy cows during the transition into lactation. We evaluated effects of feeding ethyl-cellulose rumen-protected methionine (RPM) during the periparturient period on abundance of proteins associated with transport AA and glucose, protein turnover, metabolism, and antioxidant pathways in skeletal muscle. Sixty multiparous Holstein cows were used in a block design and assigned to a control or RPM diet from -28 to 60 d in milk. The RPM was fed at a rate of 0.09% or 0.10% of dry matter intake (DMI) during the prepartal and postpartal periods to achieve a target Lys:Met ratio in the metabolizable protein of ∼2.8:1. Muscle biopsies from the hind leg of 10 clinically healthy cows per diet collected at -21, 1, and 21 d relative to calving were used for western blotting of 38 target proteins. Statistical analysis was performed using the PROC MIXED statement of SAS version 9.4 (SAS Institute Inc.) with cow as random effect, whereas diet, time, and diet × time were the fixed effects. Diet × time tended to affect prepartum DMI, with RPM cows consuming 15.2 kg/d and controls 14.6 kg/d. However, diet had no effect on postpartum DMI (17.2 and 17.1 ± 0.4 kg/d for control and RPM, respectively). Milk yield during the first 30 d in milk was also not affected by diet (38.1 and 37.5 ± 1.9 kg/d for control and RPM, respectively). Diet or time did not affect the abundance of several AA transporters or the insulin-induced glucose transporter (SLC2A4). Among evaluated proteins, feeding RPM led to lower overall abundance of proteins associated with protein synthesis (phosphorylated EEF2, phosphorylated RPS6KB1), mTOR activation (RRAGA), proteasome degradation (UBA1), cellular stress responses (HSP70, phosphorylated MAPK3, phosphorylated EIF2A, ERK1/2), antioxidant response (GPX3), and de novo synthesis of phospholipids (PEMT). Regardless of diet, there was an increase in the abundance of the active form of the master regulator of protein synthesis phosphorylated MTOR and the growth-factor-induced serine/threonine kinase phosphorylated AKT1 and PIK3C3, whereas the abundance of a negative regulator of translation (phosphorylated EEF2K) decreased over time. Compared with d 1 after calving and regardless of diet, the abundance of proteins associated with endoplasmic reticulum stress (XBP1 spliced), cell growth and survival (phosphorylated MAPK3), inflammation (transcription factor p65), antioxidant responses (KEAP1), and circadian regulation (CLOCK, PER2) of oxidative metabolism was upregulated at d 21 relative to parturition. These responses coupled with the upregulation of transporters for Lys, Arg, and His (SLC7A1) and glutamate/aspartate (SLC1A3) over time were suggestive of dynamic adaptations in cellular functions. Overall, management approaches that could take advantage of this physiological plasticity may help cows make a smoother transition into lactation.

Effects of Heat-Killed Lactobacillus plantarum L-137 Supplementation on Growth Performance, Blood Profiles, Intestinal Morphology, and Immune Gene Expression in Pigs.

In the present study, the effects of dietary heat-killed Lactobacillus plantarum L-137 (HK L-137) on the productive performance, intestinal morphology, and cytokine gene expression of suckling-to-fattening pigs were investigated. A total of 100 suckling pigs [(Large White × Landrace) × Duroc; 4.5 ± 0.54 kg initial body weight (BW)] were used and assigned to each of the four dietary treatments as follows: (1) a control diet with antibiotics as a growth promoter (AGP) from the suckling phase to the grower phase and no supplement in the finisher phases; (2) a control diet without antibiotics as a growth promoter (NAGP); (3) a control diet with HK L-137 at 20 mg/kg from the suckling phase to the starter phase and no supplement from the grower phase to the finisher phases (HKL1); and (4) a control diet with HK L-137 at 20 mg/kg from the suckling phase to the weaner phase, at 4 mg/kg from the starter phase to the finisher 1 phase, and no supplement in the finisher 2 phase (HKL2). During the weaner-starter period, the pigs fed on the AGP and HKL2 diets showed significantly higher weight gain and average daily gain (ADG) than those in the NAGP group (p < 0.05). The pigs in the AGP, HKL1, and HKL2 groups showed greater ADG than those in the NAGP groups (p < 0.05) throughout the grower-finisher period. The suckling pigs in the HKL1 and HKL2 groups showed a higher platelet count (484,500 and 575,750) than in the others (p < 0.05); however, there were no significant differences in the other hematological parameters among the treatment groups. The relative mRNA expression level of IFN- ß of the suckling and starter pigs were significantly higher in the HKL1 and HKL2 groups than in the others (p < 0.05), while the IFN-γ showed the highest level in the HKL2 suckling pigs (p < 0.05). These results demonstrate that a HK L-137 supplementation could stimulate the immune response in suckling and starter pigs and promote the growth performance in finishing pigs.

Growth performance, meat quality, and bone-breaking strength in broilers fed dietary rice hull silicon.

Bone problems have been a key issue that perilously affects broilers' health and welfare, resulting in severe economic loss. The present study was aimed at investigating the influence of dietary rice hull silicon (RHS) on the performance, meat quality, and bone-breaking strength of broilers. One hundred 10-day-old Arbor Acres chicks were used in the study. The birds were divided into 5 groups: one group was kept as the control, and other groups were provided with 2.5, 5.0, 7.5, and 10.0 mg/kg dietary RHS along with their basal diets. Results showed that diets containing various levels of dietary RHS did not adversely affect (P > 0.05) the body weight, feed intake, and feed conversion ratio. Drip loss of thigh meat showed a reduced value in the group supplemented with 7.5 mg/kg dietary RHS compared with other groups (P < 0.05), and the lowest thawing loss was observed in the same group; however, it showed no significant difference among other groups. Similarly, thawing loss of breast meat tended to decrease in the dietary RHS groups and significantly decreased (P < 0.05) in the 7.5 mg/kg RHS group. The shear force of breast meat was higher in all RHS groups, and the highest was in the 7.5 mg/kg RHS group (P < 0.05). Although tibia breaking strength increased significantly (P < 0.05) in the 7.5 mg/kg RHS group (P < 0.05), but a significant difference in femur breaking strength was not found among groups. In conclusion, dietary RHS can be used as a natural mineral supplement for improving bone-breaking strength and reducing drip and thawing loss of breast and thigh muscles, particularly RHS at a level of 7.5 mg/kg in broiler diets.

Effects of the ratio of unsaturated fatty acid to saturated fatty acid on the growth performance, carcass and meat quality of finishing pigs.

The effects on finishing pigs (80-100 kg BW) fed diets supplemented with oil sources containing different ratios of unsaturated to saturated fatty acids (UFA:SFA ratio) were evaluated in 15 barrows and 15 gilts (Duroc × Large White × Landrace). Three experimental diets were evaluated using a randomized complete block design, with broken rice, soybean meal and rice bran as the main feedstuffs in the control diet. Diets 2 and 3 consisted of the control diet supplemented with 3% oil, with UFA:SFA ratios of 2.5:1 and 5:1, respectively. Overall, there was no significant difference (P > 0.05) found in the average daily gain (ADG) of the pigs fed the treatment diets; however, the pigs fed the control diet and diet 3 had better (P < 0.05) feed conversion ratios (FCR) than the pigs fed diet 2. The pigs fed diets 2 and 3, which were supplemented with oil at UFA:SFA ratios of 2.5:1 and 5:1, had greater (P < 0.05) average daily feed intakes (ADFI) than the pigs in the control group. Additionally, it was found that the gender of the pigs had an effect (P < 0.05) on the FCR. Interaction effects between the experimental diets and the gender of the pigs (P < 0.05) were found in the ADFI and FCR. There were no significance differences (P > 0.05) among the treatment groups with regard to the carcass quality of the pigs; however, it was found that the gilts had greater (P < 0.01) loin eye areas than the barrows fed diets 2 and 3 and the loin eye area of pig fed diet 2 was the largest (P < 0.05). In the case of the meat quality parameters, it was clearly found that the pigs fed the control diet had a greater (P < 0.05) lightness (L∗) in the meat colour, and the lowest cooking loss was found in the pigs fed the diet supplemented with fat containing the UFA:SFA ratio of 5:1. Overall, the dietary treatment did not significantly affect the drip loss, thawing loss and shear force of the pork. In conclusion, the supplementation of oil with UFA:SFA ratios of 2.5:1 and 5:1 has the potential to improve pork quality.