[Adenine nucleotide metabolism in the testicular tissue of alloxan diabetic rats]. / Obmen adeninnukleotidov v tkani semennikov krys v usloviiakh alloksanovogo diabeta.

Content of ATP and AMP, total intracellular pool of adenine nucleotides, the ratio of adenylate cyclase affecting ratio of ATP/ADP, energy change of the adenylate system as well as potential of adenine nucleotides phosphorylation were decreased in testicular tissue of rats with alloxan diabetes. At the same time, content of ADP and inorganic phosphate was increased as compared with control values. The data obtained suggest that energy metabolism was distinctly impaired in rat testes under conditions of alloxan diabetes, which appears to occur as a result of decrease in AMP biosynthesis and in transformation of the nucleotide into ATP during oxidative phosphorylation. These alterations in the pool of adenylates appear to play an important role in impairments of spermatogenic and endocrine functions of testes in diabetes.

[Oxidative phosphorylation, NADH-oxidase and succinate oxidase activity in testicular mitochondria of rats with alloxan diabetes]. / Okislitel'noe fosforilirovanie, aktivnost' NADH-oksidaznoi i suktsinatoksidaznoi sistem v mitokhondriiakh testikul krys pri alloksanovom diabete.

Decrease in V4P, V3 and VP, in coefficient of respiratory control, in the ratio ADP:0 as well as an increase in duration of phosphorylation of ADP involved in oxidation of succinate were found in testes mitochondria from rats with alloxan diabetes (content of glucose 20 +/- 2 mmol/L in blood plasma). If a mixture of malate and glutamate was used as a substrate of oxidation, the rate of mitochondrial respiration and coefficient of respiratory control were decreased in testes of diabetic rats under conditions of all the metabolic states studied. At the same time, sensitivity of NADH-oxidase and succinate oxidase systems to controlled heating (28 degrees) was distinctly decreased in the testes mitochondrial membranes of the impaired animals as compared with controls. The data obtained suggest that distinct functional and, apparently, structure alterations occurred in the testes mitochondrial membranes of rats with alloxan diabetes.