RESUMO
BACKGROUND AND OBJECTIVE: Previous studies have shown that Porphyromonas gingivalis is found in the amniotic fluid and placentae of pregnant women with some obstetric diseases. However, the biological effects of P. gingivalis on intrauterine tissues remain unclear. The aim of this study was to investigate the presence of P. gingivalis in chorionic tissues from hospitalized high-risk pregnant women, and the effects of P. gingivalis lipopolysaccharide on the production of proinflammatory molecules in human chorion-derived cells. MATERIAL AND METHODS: Twenty-three subjects were selected from Japanese hospitalized high-risk pregnant women. The presence of P. gingivalis in chorionic tissues was analyzed by PCR. Cultured chorion-derived cells or Toll-like receptor-2 (TLR-2) gene-silenced chorion-derived cells were stimulated with P. gingivalis lipopolysaccharide. Real-time PCR was performed to evaluate TLR-2 and Toll-like receptor-4 (TLR-4) mRNA expression in the cells. Levels of interleukin-6 and interleukin-8 in culture supernatants of the chorion-derived cells were measured by ELISA. RESULTS: P. gingivalis DNA was detected in chorionic tissues from two women with threatened preterm labor, two with multiple pregnancy and two with placenta previa. Stimulation of chorion-derived cells with P. gingivalis lipopolysaccharide significantly increased TLR-2 mRNA expression, whereas TLR-4 mRNA expression was not changed. P. gingivalis lipopolysaccharide induced interleukin-6 and interleukin-8 production in chorion-derived cells, but the P. gingivalis lipopolysaccharide-induced interleukin-6 and interleukin-8 production was reduced in TLR-2 gene-silenced chorion-derived cells. CONCLUSION: Our results suggest that P. gingivalis can be detected in chorionic tissues of hospitalized high-risk pregnant women, and that P. gingivalis lipopolysaccharide induces interleukin-6 and interleukin-8 production via TLR-2 in chorion-derived cells.
Assuntos
Córion/microbiologia , Doenças Periodontais/microbiologia , Porphyromonas gingivalis/isolamento & purificação , Nascimento Prematuro/microbiologia , Adulto , Células Cultivadas , Córion/efeitos dos fármacos , Placa Dentária/microbiologia , Escherichia coli , Feminino , Inativação Gênica , Gengivite/classificação , Hospitalização , Humanos , Mediadores da Inflamação/análise , Interleucina-6/análise , Interleucina-8/análise , Lipopolissacarídeos/farmacologia , Perda da Inserção Periodontal/classificação , Bolsa Periodontal/classificação , Periodontite/classificação , Placenta Prévia/microbiologia , Gravidez , Gravidez de Alto Risco , Gravidez Múltipla , Saliva/microbiologia , Receptor 2 Toll-Like/análise , Receptor 2 Toll-Like/genética , Receptor 4 Toll-Like/análise , Adulto JovemRESUMO
A non-contrast-enhanced three-dimensional (3D) magnetic resonance angiography (MRA) technique, which acquires images in a reasonably short scanning time and requires no contrast agent, is developed. An electrocardiographically (ECG) synchronized 3D half-Fourier fast spin-echo (FSE) technique with an appropriate ECG delay time for every slice encoding in 3D terms was used to examine the thoracic and iliac regions in 16 healthy volunteers at both 0.5 and 1.5 T. Prior to each 3D fresh blood imaging (FBI) experiment, an ECG preparation (ECG-prep) scan was acquired, and an appropriate ECG triggering time was selected for 3D FBI acquisition to optimize visualization of the vessel of interest. In the thoracic and abdominal regions, good-quality 3D MRA images were obtained. Furthermore, the weighted subtraction of two images in different phases provides contrast enhancement between arteries and veins.
Assuntos
Eletrocardiografia/métodos , Angiografia por Ressonância Magnética/métodos , Adulto , Aorta Torácica/anatomia & histologia , Diástole , Imagem Ecoplanar/métodos , Feminino , Análise de Fourier , Humanos , Artéria Ilíaca/anatomia & histologia , Masculino , Pessoa de Meia-Idade , Artéria Pulmonar/anatomia & histologia , Valores de Referência , Sensibilidade e Especificidade , Sístole , Artérias Torácicas/anatomia & histologia , Veia Cava Superior/anatomia & histologiaRESUMO
A Fourier transform holographic memory has been developed and used for generating and storing sixteen thousand Kanji characters. The memory consists of four groups of holograms of 63 pages, recording 64 Kanji characters in each page, and has a packing density of 1.34 x 10(7) bits/cm (2). Digitized characters of sixteen sizes can be generated with natural letter faces and good image quality. This paper focuses on the recording and reconstruction of holographic Kanji memory using a pseudorandom diffuser and systematic solutions consistent with optical, mechanical, and digital systems.