RESUMO
To gain further insight into the genomic features of bovine viral diarrhea virus 1 (BVDV-1) subgenotypes, we sequenced the complete genome of BVDV-1n Shitara/02/06 and BVDV-1o IS26NCP/01. The complete genome of Shitara/02/06 and IS26NCP/01 shared 77.7 to 79.3% and 78.0 to 85.7% sequence identities with other BVDV-1 subgenotype strains, respectively.
RESUMO
In this study, we report the complete genome sequence of the bovine viral diarrhea virus 2 Japanese reference strain KZ-91CP. The complete genome comprises 12,654 nucleotides and one open reading frame with 4,020 amino acids. A 369-nucleotide-long insertion encoding the chaperone protein DnaJ is found in the nonstructural 2 (NS2) coding region.
RESUMO
In the current study, molecular, biological, and antigenic analyses were performed to characterize Border disease virus (BDV) strain FNK2012-1 isolated from a pig in 2012 in Japan. The complete genome comprises 12,327 nucleotides (nt), including a large open reading frame of 11,685 nt. Phylogenetic analysis revealed that FNK2012-1 was clustered into BDV genotype 1 with ovine strains. FNK2012-1 grew in porcine, bovine, and ovine primary cells and cell lines, but grew better in bovine and ovine cells than in porcine cells. Specific pathogen-free pigs inoculated with FNK2012-1 did not show any clinical signs. Noninoculated contact control pigs also did not show clinical signs and did not seroconvert. The results suggest that FNK2012-1 may be of ruminant origin and is poorly adapted to pigs. Such observations can provide important insights into evidence for infection and transmission of BDV, which may be of ruminant origin, among pigs.
