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1.
AIDS Res Hum Retroviruses ; 17(5): 467-74, 2001 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-11282016

RESUMO

Twenty-five recombinant (mosaic) HIV-1 genomes were detected among 151 samples comprising 118 non-B subtype sequences and 33 samples containing subtype B sequences. Seven of the 25 mosaic patterns were similar to characterized circulating recombinant forms (two A/E, four A/G, and one D/F) and one was a MAL-like A/D recombinant. Eighteen of the recombinants had evidence of subtype A sequences in at least one region of their genome. One sample was found to contain a novel recombinant form (pol F, env K). Two samples could not be characterized unambiguously as recombinant forms and a further one appeared to be a complex C/J/D/A genomic form. The majority of the mosaic genomes were recombinants between gag, pol, or env, whereas the C/J/D/A mosaic had cross-over breakpoints within pol. These findings suggest that almost 20% of non-B subtype isolates of HIV-1 circulating in the United Kingdom have mosaic genomes. This shows the diverse origin of HIV-1 strains circulating in the United Kingdom and may have implications for antiretroviral drug resistance.


Assuntos
Infecções por HIV/virologia , HIV-1/genética , Recombinação Genética , Análise por Conglomerados , Genes env , Genes gag , Genes pol , Infecções por HIV/epidemiologia , Humanos , Dados de Sequência Molecular , Filogenia , Reino Unido/epidemiologia
3.
J Virol Methods ; 87(1-2): 41-51, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10856751

RESUMO

A heteroduplex mobility assay (HMA) using 753 and 446 base pair (bp) amplicons of the p17/p24 region of the gag gene of HIV-1 has been developed and validated with reference clones and clinical samples representative of subtypes A, B, C, D, E, G, and H. There was complete concordance between the gag HMA assigned subtype and the subtype known from gag or env sequence data or env HMA. The heteroduplexes from both amplicons can be clearly resolved on either MetaPhor XR agarose or MDE polyacrylamide gels. The MetaPhor XR gel system was the more convenient and is the preferred choice for routine HMA subtyping. This gag HMA provides a rapid, simple and inexpensive method for subtyping HIV-1 based on a genomic region other than the commonly used env gene target. The incorporation of gag HMA into subtype determination algorithms should allow the detection of gag/env recombinant strains of HIV-1.


Assuntos
Genes gag/genética , HIV-1/classificação , Análise Heteroduplex/métodos , Genes env/genética , HIV-1/genética , Humanos , Ácidos Nucleicos Heteroduplexes/análise , RNA Viral/genética
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