RESUMO
Osteoarthritis is a progressive joint disease characterized by cartilage degradation and bone remodeling. Transglutaminases catalyze a calcium-dependent transamidation reaction that produces covalent cross-linking of available substrate glutamine residues and modifies the extracellular matrix. Increased transglutaminases-mediated activity is reported in osteoarthritis, but the relative contribution of transglutaminases-2 (TG2) is uncertain. We describe TG2 expression in human femoral osteoarthritis and in wild-type and homozygous TG2 knockout mice after surgically-induced knee joint instability. Increased TG2 levels were observed in human and wild-type murine osteoarthritic cartilage compared to the respective controls. Histomorphometrical but not X-ray investigation documented in osteoarthritic TG2 knockout mice reduced cartilage destruction and an increased osteophyte formation compared to wild-type mice. These differences were associated with increased TGFbeta-1 expression. In addition to confirming its important role in osteoarthritis development, our results demonstrated that TG2 expression differently influences cartilage destruction and bone remodeling, suggesting new targeted TG2-related therapeutic strategies.
Assuntos
Cartilagem/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Osteoartrite/metabolismo , Osteoartrite/cirurgia , Osteófito/metabolismo , Transglutaminases/metabolismo , Animais , Cartilagem/enzimologia , Cartilagem/patologia , Modelos Animais de Doenças , Feminino , Proteínas de Ligação ao GTP/biossíntese , Humanos , Masculino , Camundongos , Camundongos Knockout , Osteoartrite/enzimologia , Osteófito/enzimologia , Osteófito/patologia , Proteína 2 Glutamina gama-Glutamiltransferase , Fator de Crescimento Transformador beta1/biossíntese , Transglutaminases/biossínteseRESUMO
Transglutaminase (TGs) enzymes and proteins crosslinking have for long time been implicated in the formation of hard tissue development, matrix maturation and mineralization. Among the TGs family members, in the context of connective tissue formation, TG2 and Factor XIII are expressed in cartilage by hypertrophic chondrocytes. Here, we analyse the morphological consequences of TG2 deficiency, during the development of skeletal elements. When TG2 is absent, there are not gross abnormalities in the development of the skeletal system, probably from compensatory mechanisms resulting in increased expression of FXIIIA and TGF-beta 1. In vivo other TGs may be involved in promoting chondrocytes and osteoblast differentiation and matrix mineralisation.
