Effects of heavy metals and high temperature on Atlantic Forest species: Analysis of their tolerance capacity.

Changes in temperature and the deposition of potential pollutants in the soil, such as heavy metals, may damage plant communities, altering their physiological processes. High temperature may also cause a series of morpho-anatomical, physiological and biochemical changes in plants. However, tolerant plant species tend to restrict these harmful effects. The present study investigates the impact of atmospheric warming on the accumulation capacity of heavy metals (Zn, Ni, Cu) in the roots and leaves of a pioneer species (Croton floribundus) and a non-pioneer species (Esenbeckia leiocarpa) native to the Atlantic Forest of southeastern Brazil. The experimental design involved exposing the plants to two soil treatments: without excess metals (-M) and with excess metals (+M), along with varying thermoperiods of 26 °C day/19 °C night and 32 °C day/20 °C night in growth chambers. Over a 28-day period, we assessed weekly metal content, translocation, growth parameters, a non-enzymatic antioxidant (glutathione) and indicators of cell damage or oxidative stress (chlorophylls a and b, total chlorophyll (a+b), carotenoids, malondialdehyde and conjugated diene hydroperoxide contents). Both species exhibited increased metal accumulation under excess metals, employing distinct translocation strategies. C. floribundus showed high translocation rates of Ni to leaves and E. leiocarpa immobilized Ni in the roots. Atmospheric warming reduced Cu and Ni translocation from roots to leaves in both species. C. floribundus displayed lower physiological damage compared to E. leiocarpa, demonstrating robust growth. We concluded that the pioneer species possessed greater tolerance to oxidative stress induced by temperature and metal-related environmental factors than the non-pioneer species, confirming our hypothesis. In addition, our finding provides valuable insights for conservation and management of ecosystems affected by climatic and pollutant changes.

A Practical Procedure for Analysis of Lead Isotopes in Bivalve Shells Using Laser Ablation Multicollector Inductively Coupled Plasma Mass Spectrometry (LA-MC-ICP-MS).

Lead, like other trace elements, is incorporated in the growing bands of bivalve shells. The chemicals stored into the shells can provide valuable information about seawater conditions during the period of shell formation. In this study, we present a practical approach to determine Pb isotopic signatures in bivalve shells as a tool for evaluating lead pollution in coastal waters. To demonstrate the applicability of the method, Pb isotopic fingerprinting in bivalve shell layers were investigated using laser ablation multicollector inductively coupled plasma mass spectrometry (LA-MC-ICP-MS). Lead isotope ratios (208Pb/206Pb and 206Pb/207Pb) were measured along distinct sections of the maximum growth axis of the shells. Calibration and quantification of Pb isotopes were performed using NIST 612 as reference material. Our results demonstrated that Pb isotope ratios in the shells ranged from 1.143 to 1.201 for 206Pb/207Pb and from 2.061 to 2.161 for 208Pb/206Pb. The isotopic signatures recorded in the sample shells correspond to similar ranges of Pb signatures reported for marine sediments from the same study area. In general, this work shows that LA-MC-ICP-MS is a suitable technique for determining spatially resolved lead isotopic signatures in bivalve shells and that it can be used to estimate the origin of Pb pollution in aquatic environments.

Fluorescence emission spectra of target chloroplast metabolites (flavonoids, carotenoids, lipofuscins, pheophytins) as biomarkers of air pollutants and seasonal tropical climate.

Chloroplasts have luminescent metabolites-chlorophyll being the most known one-whose fluorescence emission may be a useful tool to assess the physiological status of the plant. Some antioxidants (flavonoids and carotenoids), and byproducts of membrane rupture (lipofuscins) and chlorophyll degradation (pheophytins), are chloroplasts' fluorescent metabolites directly involved in plant response to environmental stressors and pollutants and may act as a biomarker of stress. Here we hypothesized that climatic variations and air pollutants induce alterations in the emission profile of chloroplasts' fluorescent metabolites in Tillandsia usneoides (Bromeliaceae). To test this hypothesis, an active biomonitoring study was performed during 2 years in five polluted sites located at the Metropolitan Region of Campinas (São Paulo State, Brazil), aiming to identify target chloroplasts' fluorescent metabolites acting as biomarkers of environmental stress. In situ identification and quantification of the intensity of the fluorescence emission from target metabolites (flavonoids, carotenoids, lipofuscins, and pheophytins) were performed by the observation of fresh leaf sections under confocal laser scanning microscopy. Changes in the profile of fluorescence emission were correlated with local climate and air pollution data. The fluorescence emissions of flavonoids and carotenoids varied seasonally, with significant influence of rainfall and NO2. Our results expand the use of T. usneoides as a bioindicator by using alterations in the fluorescence emission profile of chloroplast metabolites. This application may be especially interesting for NO2 biomonitoring.

Effects of light, temperature and mesocarp on seed germination of Euterpe edulis (Juçara-palm) / Efeito da luz, temperatura e mesocarpo na germinação de sementes de palmito Juçara (Euterpe edulis)

The study exposed in this paper aimed at evaluating the influence of light, temperature and presence or absence of mesocarp in seeds on the germination of Euterpe edulis Mart. (juçara-palm). The germination of seeds with and without mesocarp was assessed in the presence of light (12 h photoperiod) or in the darkness, and at temperatures of 25, 28 or 30C. A complete randomized 2x2x3 (mesocarp presence, light conditions and temperatures) factorial design was used. The seed germinability and the Germination Speed Index (GSI) were evaluated. Seeds of E. edulis were indifferent either to the presence of light or to temperatures of 25, 28 and 30C. The germinability of the seeds with mesocarp was lower (6 to 10%) than those without mesocarp (47 to 75%).

O objetivo deste estudo foi avaliar a influência da luz, temperatura na germinação do fruto com ou sem mesocarpo (despolpado) de Euterpe edulis Mart. (palmiteiro-juçara). Sementes com e sem mesocarpo foram submetidas a presença de luz (fotoperíodo de 12 h) ou escuro contínuo e às temperaturas de 25, 28 ou 30 oC. O delineamento experimental foi inteiramente casualizado, em arranjo fatorial 2x2x3 (presença de mesocarpo, regimes de luz e temperaturas). Avaliou-se a germinabilidade e o índice de velocidade de germinação das sementes (IVG). As sementes de E. edulis comportaram-se indiferentes à luz e às temperaturas de 25, 28 e 30 oC. A germinabilidade das sementes com mesocarpo foi menor (6 a 10%) que das sementes sem mesocarpo (47 a 75%).

Suitability of Tillandsia usneoides and Aechmea fasciata for biomonitoring toxic elements under tropical seasonal climate.

Aechmea fasciata was evaluated for the first time as a biomonitor of toxic elements, in comparison to the biomonitoring capacity of Tillandsia usneoides, a well-established biomonitor bromeliad species. Plants of both species were exposed to air pollutants from industrial, urban, and agricultural sources, under the tropical seasonal climate, from June/2011 to April/2013, in five sites of São Paulo State, Brazil, for 8 consecutive exposure periods of 12 weeks each. The levels of essential and non-essential elements, including trace metals, were quantified at the end of each exposure. T. usneoides and A. fasciata indicated N, Fe, Zn, Co, Cr, and V as air contaminants in the studied sites, during wet and dry seasons and both species were recommended for qualitative biomonitoring. Concentration levels of N, Ca, S, Fe, Zn, Cu, B, Co, and Ni were significantly higher in T. usneoides than in A. fasciata. However, A. fasciata showed a higher effective retention capacity of Ni, Pb, V, Cu, Fe, Cr, and Co during field exposure, as indicated by the estimate of enrichment factor relative to basal concentrations. This species is more suitable for detecting the atmospheric pollution level of those metals than the T. usneoides. Both species indicated adequately the seasonal differences in the pollution levels of several elements, but T. usneoides presented higher ability for biomonitoring the spatial variations and for indicating more properly the sources of each element in the studied region than the A. fasciata.

Germinação de sementes de dalbergia nigra (Vell.) Allemao ex Benth. (Fabaceae-Papilionoideae) no armazenamento / Germination of Dalbergia nigra (Vell.) Allemao ex Benth. (Fabaceae-Papilionoideae) during storage

Dalbergia nigra (Vell.) Allemao ex Benth. (jacarandá-da-bahia), espécie de alto valor econômico pela qualidade da madeira e potencial ornamental em razão da copa bem formada e à delicadeza da folhagem, está na lista de espécies ameaçadas de extinção. Em função disso, conduziu-se este trabalho, visando à avaliação da qualidade de suas sementes, submetidas a períodos crescentes de armazenamento. Os frutos foram coletados em outubro de 2003, e as sementes armazenadas em geladeira a 6º C ± 2, em sacos de plástico transparente fechados. Mensalmente, durante 690 dias, foram retiradas 200 sementes, sendo que 100 foram utilizadas nos ensaios de germinação (quatro repetições de 25 sementes) e 100 para a determinação do teor de água. Os ensaios germinativos foram conduzidos em estufa tipo B.O.D., a 25º C e com fotoperíodo de 12 horas. As variáveis analisadas foram a porcentagem de germinação, índice de velocidade de germinação e massa seca das plântulas originadas. Os resultados indicaram que, até 360 dias de armazenamento, as sementes apresentaram porcentagem de germinação próxima de 50 por cento, com redução de cerca da metade do índice de velocidade de germinação, porém, com maior comprometimento do acúmulo da massa seca das plântulas. A porcentagem de germinação inicial e final foram de 87 por cento e 36 por cento, respectivamente. As sementes de D. nigra têm comportamento ortodoxo e podem ser armazenadas por um período de até dois anos em sacos de plástico a baixa temperatura, com redução em cerca de 50 por cento de sua capacidade.

Dalbergia nigra (Vell.) Allemao ex Benth. (Brazilian rosewood) is a highly economically valuable species because of its wood quality and ornamental potential, mainly due to its well formed canopy and the delicacy of its foliage. Unfortunately, these features contributed to list it as an endangered Brazilian species (IBAMA Regulation number 06/92). This work had as its objective to study the quality of Dalbergia nigra seeds submitted to increasing storage periods. Fruits from trees located at the Botanical Institute of Sao Paulo were collected in October 2003. After harvested, the fruits were sun dried and the seeds manually separated. The seeds were stored in closed transparent plastic bags under refrigeration (6º C ± 2). Monthly, during 690 days, 200 seeds were removed from the refrigerator. One hundred (100) seeds were used in germination tests, which consisted of four repetitions with 25 seeds each; and the other 100 were used in the determination of water content. The germination tests were conducted in a B.O.D. chamber at 25º C under a 12 hour photoperiod. The analyzed variables were; the germination percentage, germination velocity index, and dry mass of the seedlings. The results indicated that after 360 days of storage, the seeds showed germination percentage close to 50 percent and reduction of the germination velocity index to half, whereas the dry mass accumulation was the most compromised. The initial and final germination percentages were 87 percent and 36 percent, respectively. We concluded that Dalbergia nigra seeds present orthodox behavior and can be stored for a period of approximately two years in plastic bags at low temperature, compromising 50 percent of their germination capacity.

Reguladores vegetais e teores endógenos de poliaminas durante o desenvolvimento de taro cultivado in vitro / Plant growth regulators in polyamines endogenous levels during the development of taro cultivated in vitro

Neste trabalho foi investigada a assepsia para obtenção de explantes oriundos de tubérculos e a ação das poliaminas espermidina e espermina exógenas associadas aos reguladores vegetais AIA e BA no desenvolvimento, na tuberização in vitro e nos níveis endógenos de putrescina (Put), espermidina (Spd) e espermina (Spm) de taro (Colocasia esculenta). Plantas crescidas em meio contendo espermidina e espermina mostraram tuberização e a associação dessas poliaminas com AIA e BA induziu aumento do número de brotações. Para o estímulo da rizogênese, não foi necessário o uso de reguladores vegetais. Altos teores de putrescina foram encontrados durante a emissão de brotações, enquanto que altos teores de espermidina foram observados durante a formação de rizomas in vitro.

The present research investigated the asepsis for attainment of explants deriving of tubers and the polyamines espermidine and espermine exogenous effects associated with the plant growth regulators NAA and BA on the development and tuberization in vitro and the endogenous levels of putrescine (Put), spermidine (Spd) and spermine (Spm) of taro (Colocasia esculenta). Plants grown in the medium with spermidine and spermine showed tuberization and the association of these polyamines with NAA and BA increases number of shoots. Plant growth regulators were not necessary for root initiation. High levels of endogenous Put were found during the shoot emission, while high levels Spd were observed during in vitro root formation.