ISOLATION AND COMPERATIVE STUDY OF THE GROWTH INHIBITING THERMOSTABLE PROTEIN COMPLEX FROM THE BONE MARROW OF THE ADULT MICE.

The growth inhibiting thermostable protein complex (TPC) from the bone marrow cells of adult mice was isolated and partially characterized. A comparative analysis of TPC components was performed by polyacrylamide gel electrophoresis. It has been established that the complex isolated from the bone marrow of adult mice, like complexes obtained from other organs, contains two relatively high-molecular-weight and low-molecular-weight sub fractions of proteins. In addition, minor content of low molecular weight components was detected in bone marrow TPC. It has been established that the thermostable protein complex of adult mice bone marrow cells does not have the ability to inhibit the proliferation of homotypic cells. This may be due to the minor content of active component (low molecular weight subfraction) in the complex and the structural and functional properties of constantly renewable hematopoietic tissue, such as the nature of factors that regulate proliferation, their different cell penetration and the expression of membrane receptors.

THE EFFECT OF EXTRACT OF GREEK WALNUT (JUGLANS REGIA L.) SEPTA ON SOME FUNCTIONAL CHARACTERISTICS OF ERYTHROCYTES.

Administration of plant extracts for the treatment of several different diseases is an important approach ofmodern medicine.The reason must be an easy way of application, low price and the complex action of herbal medicines. The aim of the work was to study the effect of extract of walnut (Juglans regia L.) septa on the functional characteristics of erythrocytes during administration of cytotoxic agent - cyclophosphamide (experimental model of leukopenia). The material for the study was blood of the intact and experimental white mice. Sorption capacity and resistance to lysis of erythrocyte membrane have been determined by the spectrophotometric methods. According to the gained results, administration of cyclophosphamide had an influence on sorption capacity of erythrocytes and the given characteristic was increased only on the 8th day of cyclophosphamide administration, compared to control. Sorption capacity of erythrocytes was increased more on 8th day after combined application of cyclophosphamide and exctract of walnut septa. Resistance of erythrocytes to lysis was also increased after administration of the cyclophosphamide and this characteristic was further increased in case of combined application of cyclophosphamide and exctract of walnut septa. Thus, the stimulative effects of extract of walnut septa have been established on sorption capacity and resistance to lysis of erythrocytes in case of nonspecific damage of the cells.