Analysis of torso movement of trans-femoral amputees during level walking.

The purpose of this study is to analyze the movement of unilateral trans-femoral amputees' gait and find patterns of compensated movement to accommodate the loss of locomotor power on one side. A 3-D analyzer system and force plate were used to measure 12 amputees. The main focus was to find characterized movement particularly of the upper body such as pelvis, shoulder and arms. It was Saunders et al. (1953), who said that the human body can be purposely divided into two subsystems in analyzing man's locomotion. This study, however is not to measure the level of function, but to evaluate the appearance of gait. Twelve markers were placed on the subjects, and two locations for measuring points were used to measure movement of the torso in three axis (X, Y, Z) without measuring the position of the centre of gravity. The two points were at shoulder level, and at pelvic level. Lissajou's figure was used to evaluate the movements at these two marks. The quality of the gait was determined through subjective evaluation by the author. Determination was made on two factors, one from observing the gait of the amputees and the other, patterns from their Lissajou's figure of two measuring points. Then they were categorized as good walker or "other". Good walkers had results at shoulder level which were closer to the symmetrical Lissajou's figure of normal walkers. Yet the symmetrical pattern was not present at the pelvis level for the good walker.

D-galactoside-specific lectins from the body wall of an echiuroid (Urechis unicinctus) and two annelids (Neanthes japonica and Marphysa sanguinea).

Lectins recognizing D-galactosides were purified from the body wall of an echiuroid; Urechis unicinctus and two annelids; Neanthes japonica and Marphysa sanguinea, with single step lactosyl-agarose affinity column chromatography. SDS-PAGE under reduced and non-reduced conditions showed that U. unicinctus lectin had a major (36 kDa) and two minor (40 and 14 kDa) proteins, and that N. japonica lectin and M. sanguinea lectin had single 33 and 35 kDa proteins, respectively. Lectins were solubilized in the presence of lactose from tissues, and all polypeptides were shown to have sugar binding activity. The antisera raised against U. unicinctus lectin and N. japonica lectin crossreacted with each other but did not crossreact with bull frog (Rana catesbeiana) egg galectin-1 or a D-galactoside-specific lectin purified from sea urchin (Anthocidaris crassispina) eggs. These echiuroid and annelid lectins are immunologically similar, but distinct from members of the vertebrate galectin family.

Activating effect of light irradiation at various wavelength on the respiration in sperm of the echiuroid, Urechis unicinctus, in the presence of carbon monoxide.

In sperm of the echiuroid, Urechis unicinctus, respiration in the presence of CO was reversibly augmented by light irradiation in an examined range of wavelengths between 350 and 650 nm. The respiratory rate of sperm in the presence of CO was enhanced by light irradiation in proportion to the light fluence rate. A sharp and large peak was obtained at the wavelength of 430 nm in the action spectrum of photo-activated respiration of sperm in the presence of CO. Broad and small peaks were also found at around 530 and 570 nm. This action spectrum is similar in its profile to the absorption spectrum of reduced cytochrome b. Absorption of photon energy by reduced b-type cytochrome probably activates the redox reaction of this cytochrome to enhance the respiratory rate. Photo-activated respiration in the presence of CO was inhibited by antimycin A and cyanide. In this respiratory system, an electron equivalent is probably transferred through the mitochondrial respiratory chain between cytochrome b and cytochrome c and finally to molecular oxygen in the reaction catalyzed by the CO-insensitive terminal oxidase.