[Induction of callus of Hypericum perforatum L. and qualitative identification of active constituents of its callus].

OBJECTIVE: To induce the callus of H. perforatum and identify hypericin and pseudohypericin of its callus. METHOD: The callus was induced in different culture conditions, and active constituents were determined by HPLC. RESULT AND CONCLUSION: The inductions of callus from different parts were discussed, the induction rate of the leaf axil being the highest. The MS basic medium with 4 micrograms.L-1 2,4-D and 0.2 microgram.L-1 6-BA was the best of all screened media. Hypericin in the callus is determined by HPLC.