RESUMO
Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 µM)-induced platelet aggregation (ca 80% inhibition). Attempts to measure the antiplatelet aggregation activity of crude Echis carinatus venom and its fractions were not successful due to the protein coagulation of the plasma samples after the addition of venom. Anticoagulant activities of venoms were also evaluated. Total venom of Echis carinatus showed anti coagulant activity in PT test, while its fractions showed procoagulant activity.
Assuntos
Aorta Torácica/lesões , Ruptura Aórtica/complicações , Oxigenação por Membrana Extracorpórea/métodos , Traumatismo Múltiplo/complicações , Síndrome do Desconforto Respiratório , Acidentes de Trânsito , Doença Aguda , Adulto , Ruptura Aórtica/diagnóstico , Cuidados Críticos/métodos , Ecocardiografia Transesofagiana , Embolia Gordurosa/complicações , Fraturas do Fêmur/complicações , Traumatismos do Pé/complicações , Traumatismos do Antebraço/complicações , Fraturas Cominutivas/complicações , Humanos , Cuidados para Prolongar a Vida/métodos , Masculino , Síndrome do Desconforto Respiratório/etiologia , Síndrome do Desconforto Respiratório/terapia , Costelas/lesões , Ruptura , Tomografia Computadorizada por Raios X , Traumatologia/métodos , Resultado do TratamentoRESUMO
BACKGROUND AND AIM OF THE STUDY: The safety and efficacy of beating-heart valve surgery as a myocardial protection strategy was evaluated in patients with renal failure requiring hemodialysis. METHODS: This was a retrospective review of nine patients (four males, five females; mean age 46.7 years; mean duration of hemodialysis 47 +/- 49 months) who underwent beating-heart valve surgery at the present authors' institution between April 2000 and September 2002. RESULTS: The mean cardiopulmonary bypass time was 77.2 +/- 8 min. Perioperatively, two patients died (one from sepsis; one from complication of anticoagulation). There were no deaths in the follow up since discharge, with average follow up 18.3 months (range: 9-27 months). Other complications included reintubation for <24 h (one case), AV graft thrombosis (one patient) and stroke (one patient, as mentioned above). There were no new cardiac (including arrhythmia and low cardiac output syndrome) or metabolic complications (including hyperkalemia and fluid overload). CONCLUSION: This is the first report of beating-heart valve surgery using simultaneous antegrade and retrograde perfusion with normothermic blood. Despite being small in size, the study demonstrated the safety of this approach in a high-risk population with renal failure requiring hemodialysis. The results suggested a low incidence of complications, and short ICU and hospital stays.
