Genetic Diversity of Legionella pneumophila Isolates from Artificial Water Sources in Brazil.

Legionella pneumophila (Lp) is a Gram-negative bacterium found in natural and artificial aquatic environments and inhalation of contaminated aerosols can cause severe pneumonia known as Legionnaires' Disease (LD). In Brazil there is hardly any information about this pathogen, so we studied the genetic variation of forty Legionella spp. isolates obtained from hotels, malls, laboratories, retail centers, and companies after culturing in BCYE medium. These isolates were collected from various sources in nine Brazilian states. Molecular identification of the samples was carried out using Sequence-Based Typing (SBT), which consists of sequencing and analysis of seven genes (flaA, pilE, asd, mip, mompS, proA, and neuA) to define a Sequence Type (ST). Eleven STs were identified among 34/40 isolates, of which eight have been previously described (ST1, ST80, ST152, ST242, ST664, ST1185, ST1464, ST1642) and three were new STs (ST2960, ST2962, and ST2963), the former identified in five different cooling towers in the city of São Paulo. The ST1 that is widely distributed in many countries was also the most prevalent in this study. In addition, other STs that we observed have also been associated with legionellosis in other countries, reinforcing the potential of these isolates to cause LD in Brazil. Unfortunately, no human isolates could be characterized until presently, but our observations strongly suggest the need of surveillance implementation system and control measures of Legionella spp. in Brazil, including the use of more sensitive genotyping procedures besides ST.

In-Laboratory Polysomnography Worsens Obstructive Sleep Apnea by Changing Body Position Compared to Home Testing.

(1) Background: Home sleep apnea testing, known as polysomnography type 3 (PSG3), underestimates respiratory events in comparison with in-laboratory polysomnography type 1 (PSG1). Without head electrodes for scoring sleep and arousal, in a home environment, patients feel unfettered and move their bodies more naturally. Adopting a natural position may decrease obstructive sleep apnea (OSA) severity in PSG3, independently of missing hypopneas associated with arousals. (2) Methods: Patients with suspected OSA performed PSG1 and PSG3 in a randomized sequence. We performed an additional analysis, called reduced polysomnography, in which we blindly reassessed all PSG1 tests to remove electroencephalographic electrodes, electrooculogram, and surface electromyography data to estimate the impact of not scoring sleep and arousal-based hypopneas on the test results. A difference of 15 or more in the apnea-hypopnea index (AHI) between tests was deemed clinically relevant. We compared the group of patients with and without clinically relevant differences between lab and home tests (3) Results: As expected, by not scoring sleep, there was a decrease in OSA severity in the lab test, similar to the home test results. The group of patients with clinically relevant differences between lab and home tests presented more severe OSA in the lab compared to the other group (mean AHI, 42.5 vs. 20.2 events/h, p = 0.002), and this difference disappeared in the home test. There was no difference between groups in the shift of OSA severity by abolishing sleep scoring in the lab. However, by comparing lab and home tests, there were greater variations in supine AHI and time spent in the supine position in the group with a clinically relevant difference, either with or without scoring sleep, showing an impact of the site of the test on body position during sleep. These variations presented as a marked increase or decrease in supine outcomes according to the site of the test, with no particular trend. (4) Conclusions: In-lab polysomnography may artificially increase OSA severity in a subset of patients by inducing marked changes in body position compared to home tests. The location of the sleep test seems to interfere with the evaluation of patients with more severe OSA.

The influence of the Great Recession on perinatal health-an ecological study on the trend changes and regional differences in Portugal.

Background: Few studies examine the relationship between socioeconomic factors and trends in mortality in high-income European countries. Due to the lack of regional-level data, most recent studies on social inequality in Portugal do not investigate regional differences. This study analyses time trends and regional disparities in the evolution of perinatal mortality (PMR) and infant mortality (IMR) associated with demographic and socioeconomic indicators following Portugal's 2008 economic and financial crisis. Methods: Associations were assessed using generalised linear models. A Poisson joinpoint regression model was applied to identify relevant PMR and IMR changes between 2000 and 2018. Country regional disparities were analysed using Mixed Effect Multilevel models. Findings: IMR and PMR significantly decreased in the pre-crisis period but not in the post-crisis period. The significant differences between regions in IMR and PMR in 2000 were followed by a different evolution of regional IMR after 2008. PMR and IMR were not significantly associated with socioeconomic indicators. A significant positive association with maternal age at first birth was identified. Interpretation: Results confirm the influence of the crisis on PMR and IMR trends in Portugal, taking into account recurring associations between macroeconomic cycles, variations in mortality trends, macroeconomic volatility, and stagnation of IMR and PMR. Regional inequalities confirm the internal variability of the crisis influence and persistent spatial inequalities affecting IMR patterns. Funding: FCT, under the Institute of Public Health of the University of Porto (ISPUP)-EPIUnit (UIDB/04750/2020) and ITR (LA/P/0064/2020), Maastricht University's external PhD programme under the Care and Public Health Research Institute (CAPHRI), and the RECAP preterm project (grant agreement no 733280).

Human N-acetyltransferase 2 (NAT2) gene variability in Brazilian populations from different geographical areas.

Introduction: Several polymorphisms altering the NAT2 activity have already been identified. The geographical distribution of NAT2 variants has been extensively studied and has been demonstrated to vary significantly among different ethnic population. Here, we describe the genetic variability of human N-acetyltransferase 2 (NAT2) gene and the predominant genotype-deduced acetylation profiles of Brazilians. Methods: A total of 964 individuals, from five geographical different regions, were genotyped for NAT2 by sequencing the entire coding exon. Results: Twenty-three previously described NAT2 single nucleotide polymorphisms (SNPs) were identified, including the seven most common ones globally (c.191G>A, c.282C>T, c.341T>C, c.481C>T, c.590G>A, c.803A>G and c.857G>A). The main allelic groups were NAT2*5 (36%) and NAT2*6 (18.2%), followed to the reference allele NAT2*4 (20.4%). Combined into genotypes, the most prevalent allelic groups were NAT2*5/*5 (14.6%), NAT2*5/*6 (11.9%) and NAT2*6/*6 (6.2%). The genotype deduced NAT2 slow acetylation phenotype was predominant but showed significant variability between geographical regions. The prevalence of slow acetylation phenotype was higher in the Northeast, North and Midwest (51.3%, 45.5% and 41.5%, respectively) of the country. In the Southeast, the intermediate acetylation phenotype was the most prevalent (40.3%) and, in the South, the prevalence of rapid acetylation phenotype was significantly higher (36.7%), when compared to other Brazilian states (p < 0.0001). Comparison of the predicted acetylation profile among regions showed homogeneity among the North and Northeast but was significantly different when compared to the Southeast (p = 0.0396). The Southern region was significantly different from all other regions (p < 0.0001). Discussion: This study contributes not only to current knowledge of the NAT2 population genetic diversity in different geographical regions of Brazil, but also to the reconstruction of a more accurate phenotypic picture of NAT2 acetylator profiles in those regions.

Dietary Habits and Gaming Behaviors of Portuguese and Brazilian Esports Players.

As the esports industry grows, marketing campaigns for fast food, soft drinks, alcoholic and energy drinks, and dietary supplements at related events intensify. Portuguese and Brazilian esports players' dietary patterns remain unexplored. This study aimed to characterize the dietary and gaming habits of esports players. We applied an anonymous, open online survey to a convenience sample of esports players that included the PREDIMED and the Internet Gaming Disorder Scale. The survey was shared through email and social media platforms, including Facebook, Twitter, Instagram, Discord, WhatsApp, and Twitch. The sample (n = 579) was predominantly male (91.4%), averaged 26.1 (SD = 7.0) years, and 25.9 (SD = 5.5) kg/m2. Most reported a weak (53.7%) adherence to the Mediterranean diet. Almost a third (32.3%) consumed dietary supplements. Our sample presented a low adhesion to the Mediterranean dietary pattern, low consumption of fruit and vegetables, and high consumption of fast food, red and processed meat, soft drinks, and dietary supplements, including caffeine-based supplements. Dietary supplement consumption was related to a higher adhesion to the Mediterranean diet, and a higher level of professionalization and internet gaming disorder correlated with a lower adhesion. In conclusion, we infer that Portuguese and Brazilian esports players follow an unbalanced diet.

Combined Use of a Bacterial Consortium and Early-Colonizing Plants as a Treatment for Soil Recovery after Fire: A Model Based on Los Guájares (Granada, Spain) Wildfire.

During 2022, intense heat waves, together with particularly extreme dry conditions, created a propitious scenario for wildfires, resulting in the area of vegetation consumed in Europe doubling. Mediterranean countries have been particularly affected, reaching 293,155 hectares in Spain, the worst data in the last 15 years. The effects on the vegetation and the soil are devastating, so knowing the recovery factors is essential for after-fire management. Resilient microorganisms play a fundamental role in rapid nutrient recycling, soil structure, and plant colonization in fire-affected soils. In this present work, we have studied emergent microbial communities in the case of the Los Guájares (Granada, Spain) fire, one of the most extensive of the year, to evaluate their role in the recovery of soil and vegetation cover. We aim to discern which are the main actors in order to formulate a new treatment that helps in the ecosystem recovery. Thus, we have found the relevant loss in phosphorous and potassium solubilizers, as well as siderophores or biofilm producers. Here, we decided to use the strains Pseudomonas koreensis AC, Peribacillus frigoritolerans CB, Pseudomonas fluorescens DC, Paenibacillus lautus C, Bacillus toyonensis CD, and Paenarthrobacter nitroguajacolicus AI as a consortium, as they showed most of the capacities required in a regenerative treatment. On the other hand, the microcosm test showed an enhanced pattern of germination of the emerging model plant, Bituminaria bituminosa, as well as a more aggregated structure for soil. This new approach can create a relevant approach in order to recover fire-affected soils in the future.

Lifestyle and eating habits of bank employees in Mozambique / Estilo de vida y hábitos alimenticios de banqueros em Mozambique / Estilo de vida e hábitos alimentares de bancários em Moçambique

Objective: to evaluate the lifestyle and eating habits of bank workers in Mozambique. Method: a cross-sectional study with a quantitative approach, with 32 employees from three bank branches, selected through convenience sampling, in 2021. A food frequency questionnaire and a lifestyle questionnaire were applied. Descriptive statistics were employed for data treatment using SPSS v.25, presenting the data as absolute and relative frequencies. Results: in terms of lifestyle, 65.6% of participants were physically active, 100% were non-smokers, and 40.6% preferred fruits and vegetables instead of sweet and savory foods. Regarding food consumption frequency, the bread and equivalents group had the highest daily intake, with French bread (44%) being the most consumed, followed by the fruit and vegetable group with lettuce leading consumption (44%). The third group was meat and equivalents with the most consumed food in that category being fried egg (28%). The least consumed food group was oils and sauces with the most consumed in the category being salad dressings (19%). Conclusion: more healthy lifestyles and eating habits were evidenced.

Using Digital Tools to Study the Health of Adults Born Preterm at a Large Scale: e-Cohort Pilot Study.

BACKGROUND: Preterm birth is a global health concern. Its adverse consequences may persist throughout the life course, exerting a potentially heavy burden on families, health systems, and societies. In high-income countries, the first children who benefited from improved care are now adults entering middle age. However, there is a clear gap in the knowledge regarding the long-term outcomes of individuals born preterm. OBJECTIVE: This study aimed to assess the feasibility of recruiting and following up an e-cohort of adults born preterm worldwide and provide estimations of participation, characteristics of participants, the acceptability of questions, and the quality of data collected. METHODS: We implemented a prospective, open, observational, and international e-cohort pilot study (Health of Adult People Born Preterm-an e-Cohort Pilot Study [HAPP-e]). Inclusion criteria were being an adult (aged ≥18 years), born preterm (<37 weeks of gestation), having internet access and an email address, and understanding at least 1 of the available languages. A large, multifaceted, and multilingual communication strategy was established. Between December 2019 and June 2021, inclusion and repeated data collection were performed using a secured web platform. We provided descriptive statistics regarding participation in the e-cohort, namely, the number of persons who registered on the platform, signed the consent form, initiated and completed the baseline questionnaire, and initiated and completed the follow-up questionnaire. We also described the main characteristics of the HAPP-e participants and provided an assessment of the quality of the data and the acceptability of sensitive questions. RESULTS: As of December 31, 2020, a total of 1004 persons had registered on the platform, leading to 527 accounts with a confirmed email and 333 signed consent forms. A total of 333 participants initiated the baseline questionnaire. All participants were invited to follow-up, and 35.7% (119/333) consented to participate, of whom 97.5% (116/119) initiated the follow-up questionnaire. Completion rates were very high both at baseline (296/333, 88.9%) and at follow-up (112/116, 96.6%). This sample of adults born preterm in 34 countries covered a wide range of sociodemographic and health characteristics. The gestational age at birth ranged from 23+6 to 36+6 weeks (median 32, IQR 29-35 weeks). Only 2.1% (7/333) of the participants had previously participated in a cohort of individuals born preterm. Women (252/333, 75.7%) and highly educated participants (235/327, 71.9%) were also overrepresented. Good quality data were collected thanks to validation controls implemented on the web platform. The acceptability of potentially sensitive questions was excellent, as very few participants chose the "I prefer not to say" option when available. CONCLUSIONS: Although we identified room for improvement in specific procedures, this pilot study confirmed the great potential for recruiting a large and diverse sample of adults born preterm worldwide, thereby advancing research on adults born preterm.

Conhecimento dos estudantes de enfermagem sobre biossegurança no contexto hospitalar na pandemia: abordagem qualitativa / Nursing students' knowledge about biosafety in the hospital context during the pandemic: a qualitative approach

OBJETIVO: Descrever o conhecimento dos estudantes de enfermagem sobre as medidas de biossegurança no contexto hospitalar em tempos de pandemia. MÉTODO: Estudo descritivo, exploratório, qualitativo, com estudantes de enfermagem de uma universidade pública da baixada litorânea do Rio de Janeiro, Brasil, através de entrevista em ambiente virtual, entre julho e setembro de 2021. Utilizou-se o Interface de R pour Analyses Multidimensionnelles de Textes Et de Questionnaires para processamento do corpus textual e Análise de Conteúdo Temática para interpretação das falas. RESULTADOS: Participaram 29 estudantes que reconhecem a biossegurança, mas não possuem segurança ao abordá-la. Para eles, trata-se de normas para proteção do trabalhador e pacientes, associando-as aos EPIs e ao uso durante a pandemia. CONCLUSÃO: A biossegurança não é reconhecida na sua totalidade entre estudantes de enfermagem que estarão no mercado de trabalho futuramente. É necessário um olhar ampliado em perspectiva transversal, especialmente nas disciplinas do ciclo profissionalizante da enfermagem.

OBJECTIVE: To describe Nursing students' knowledge about the biosafety measures in the hospital context in pandemic times. METHOD: A descriptive, exploratory and qualitative study conducted through interviews in a virtual environment between July and September 2021 with Nursing students from a public university in the coastal lowland of Rio de Janeiro, Brazil. Interface de R pour Analyses Multidimensionnelles de Textes Et de Questionnaires was used to process the text corpus and Thematic Content Analysis was employed to interpret the testimonies. RESULTS: The participants were 29 students that recognize biosafety but are not confident enough to address it. For them, it is but a set of standards for the protection of workers and patients alike, associating it with PPE and with its use during the pandemic. CONCLUSION: Biosafety is not recognized in its entirety by the Nursing students who will enter the labor market in the near future. An expanded and cross-sectional perspective is required, especially in the academic disciplines of Nursing training cycle.

Contribuição de familiares na idealização de cartilha educativa sobre cuidados à criança após alta hospitalar / Contribución de los familiares en la creación de un folleto educativo sobre el cuidado del niño tras el alta hospitalaria / Contribution of family members in the idealization of an educational booklet on child care after hospital discharge

Objetivo: descrever as contribuições de familiares na idealização de cartilha educativa sobre cuidados domiciliares prestados à criança após a alta hospitalar. Método: pesquisa qualitativa, desenvolvida em hospital público no interior do estado do Rio de Janeiro, por meio de roteiro semiestruturado com 30 familiares de crianças hospitalizadas, cujos dados foram processados no Software IRAMUTEQ. Resultados: o agrupamento em classes permitiu o elenco de tópicos a serem abordados na cartilha educativa, a saber: cuidado com a criança em si, ambiente domiciliar, roupa, banho, medicação, animal de estimação, tapetes, cortinas, identificação de quadros respiratórios, sintomas e da alergia à proteína do leite. Ademais, familiares almejam ser acolhidos pelos profissionais de saúde e a cartilha deve apresentar informações simplificadas, lúdicas e convidativas. Conclusão: os depoimentos dos familiares contribuem para construção de uma tecnologia educacional em saúde pautada nas necessidades da população-alvo, facilitando a continuidade do cuidado no domicílio após a alta hospitalar.

Objective: to describe the contributions of family members in the creation of an educational booklet on home care provided to children after hospital discharge. Method: qualitative research, developed in a public hospital in the countryside of the state of Rio de Janeiro, through a semi-structured script with 30 family members of hospitalized children, whose data were processed in the IRAMUTEQ Software. Results: the grouping into classes allowed the list of topics to be addressed in the educational booklet, namely: care for the child, home environment, clothing, bath, medication, pet, carpets, and curtains, identification of respiratory conditions, symptoms and allergy to milk protein. In addition, family members seek to be welcomed by health professionals and the booklet should present simplified, playful and inviting information. Conclusion: the testimonies of family members contribute to the construction of an educational health technology based on the needs of the target population, facilitating the continuity of care at home after hospital discharge.

Objetivo: describir las contribuciones de los familiares en la creación de un folleto educativo sobre los cuidados domiciliarios brindados a los niños después del alta hospitalaria. Método: investigación cualitativa, desarrollada en un hospital público del interior del estado de Río de Janeiro, mediante un guión semiestructurado con 30 familiares de niños hospitalizados, cuyos datos fueron procesados ​​mediante el software IRAMUTEQ. Resultados: la agrupación en clases permitió abordar la lista de temas a tratar en el folleto educativo, a saber: cuidado del niño en si, ambiente del hogar, vestimenta, baño, medicamentos, mascota, alfombras, cortinas, identificación de afecciones respiratorias, síntomas y alergia a la proteína de la leche. Además, los familiares quieren ser bien recibidos por los profesionales de la salud y el folleto debe presentar información simplificada, divertida y atractiva. Conclusión: los testimonios de los familiares contribuyen para la construcción de una tecnología educativa en salud basada en las necesidades de la población objetivo, facilitando la continuidad de la atención en el domicilio después del alta hospitalaria.

Fatores relacionados à gravidez na adolescência: perfil reprodutivo de um grupo de gestantes / Factors related to pregnancy in adolescence: reproductive profile of a group of pregnant women / Factores relacionados con el embarazo en la adolescencia: perfil reproductivo de un grupo de mujeres embarazadas

Objetivo: descrever o perfil reprodutivo de mulheres adolescentes participantes de um grupo de gestantes. Método: estudo descritivo, transversal, documental e retrospectivo realizado através da ficha de cadastro de participantes de um grupo de gestante vinculado ao consultório de enfermagem de uma universidade pública federal do rio de janeiro em 2018. Resultados: analisou-se 59 cadastros. houve predominância de mulheres, jovens (71,2%); solteiras (72,3%); multíparas (56%); que tiveram cesárea como via de parto anteriormente (39%); no segundo trimestre de gestação (61%); tipo de pré-natal público (86,4%); desejando a via de parto vaginal (45,8%) e laqueadura pós-parto como método contraceptivo (30,5%), participaram do grupo sem acompanhantes (79,7%) e desejam visita domiciliar pós-parto (78%). Conclusão: identificou-se a necessidade, fatores relacionados e vulnerabilidades em saúde reprodutiva com vistas a implementação de cuidados primários voltados à promoção da saúde, prevenção de agravos e detecção precoce.

Objective: to describe the reproductive profile of adolescent women participating in a group of pregnant women. Method:descriptive, cross-sectional, documentary and retrospective study carried out through the registration form of participants of a group of pregnant women linked to the nursing office of a federal public university in Rio de Janeiro in 2018. Results: 59 records were analyzed. there was a predominance of women, young people (71.2%); single (72.3%); multiparous (56%); who had previously had a cesarean section (39%); in the second trimester of pregnancy (61%); type of public prenatal care (86.4%); desiring vaginal delivery (45.8%) and postpartum tubal ligation as a contraceptive method (30.5%), participated in the group without companions (79.7%) and desired postpartum home visit (78%). Conclusion: the need, related factors and vulnerabilities in reproductive health were identified with a view to implementing primary care aimed at health promotion, disease prevention and early detection.

Objetivo: describir el perfil reproductivo de mujeres adolescentes participantes de un grupo de gestantes. Método: estudio descriptivo, transversal, documental y retrospectivo realizado a través del formulario de registro de participantes de un grupo de gestantes vinculadas al consultorio de enfermería de una universidad pública federal de Río de Janeiro en 2018. Resultados: se analizaron 59 registros. hubo predominio de mujeres, jóvenes (71,2%); soltero (72,3%); multíparas (56%); que previamente había tenido una cesárea (39%); en el segundo trimestre del embarazo (61%); tipo de atención prenatal pública (86,4%); deseando parto vaginal (45,8%) y ligadura de trompas posparto como método anticonceptivo (30,5%), participaron del grupo sin acompañantes (79,7%) y desearon visita domiciliaria posparto (78%). Conclusión: se identificaron la necesidad, los factores relacionados y las vulnerabilidades en salud reproductiva para la implementación de la atención primaria dirigida a la promoción de la salud, la prevención de enfermedades y la detección temprana.

Development of mask design as personal protective equipment through 3D printing.

This project aimed to develop an innovative mask design, aided by 3D printing technology that was able to protect its wearers against virus infection. The project was motivated with the intention of helping to fight Covid-19, but that its use was extensive as an option to N95 and similar masks, already on the market, even after the pandemic. Whose main design objective was that the mask had a sealing efficiency based on a half-face mask and that it could be used in conjunction with masks made of antiviral fabrics, such as, for example, a surgical mask that has its filtering capacity already tested and approved and, which would be the filter element of the mask. The mask was also intended to be reusable so that it could be washed for cleaning, with only the fabric filter element being disposable.

Machine learning methods to predict attrition in a population-based cohort of very preterm infants.

The timely identification of cohort participants at higher risk for attrition is important to earlier interventions and efficient use of research resources. Machine learning may have advantages over the conventional approaches to improve discrimination by analysing complex interactions among predictors. We developed predictive models of attrition applying a conventional regression model and different machine learning methods. A total of 542 very preterm (< 32 gestational weeks) infants born in Portugal as part of the European Effective Perinatal Intensive Care in Europe (EPICE) cohort were included. We tested a model with a fixed number of predictors (Baseline) and a second with a dynamic number of variables added from each follow-up (Incremental). Eight classification methods were applied: AdaBoost, Artificial Neural Networks, Functional Trees, J48, J48Consolidated, K-Nearest Neighbours, Random Forest and Logistic Regression. Performance was compared using AUC- PR (Area Under the Curve-Precision Recall), Accuracy, Sensitivity and F-measure. Attrition at the four follow-ups were, respectively: 16%, 25%, 13% and 17%. Both models demonstrated good predictive performance, AUC-PR ranging between 69 and 94.1 in Baseline and from 72.5 to 97.1 in Incremental model. Of the whole set of methods, Random Forest presented the best performance at all follow-ups [AUC-PR1: 94.1 (2.0); AUC-PR2: 91.2 (1.2); AUC-PR3: 97.1 (1.0); AUC-PR4: 96.5 (1.7)]. Logistic Regression performed well below Random Forest. The top-ranked predictors were common for both models in all follow-ups: birthweight, gestational age, maternal age, and length of hospital stay. Random Forest presented the highest capacity for prediction and provided interpretable predictors. Researchers involved in cohorts can benefit from our robust models to prepare for and prevent loss to follow-up by directing efforts toward individuals at higher risk.

Increased interaction and procedural flexibility favoured participation: Study across European cohorts of preterm born individuals.

OBJECTIVE: To understand participation and attrition phenomena variability in European cohorts of individuals born preterm through in-depth exploration of the interplay of situational elements involved. METHODS: Multi-situated qualitative design, using focus groups, semi-structured interviews and collaborative visual methodology with a purposive sample of adults born preterm, parents and professionals (n = 124) from eight cohorts in seven European countries. RESULTS: Most cohort participants were motivated by altruism/solidarity and gratitude/sense of duty to reciprocate (only absent in adults aged 19 - 21), followed by expectation of direct benefit to one's health and knowledge amongst participating adults. Common deterrents were perceived failure in reciprocity as in insufficient/inadequate interaction and information sharing, and postal questionnaires. Combining multipurpose, flexible strategies for contact and assessment, reminders, face-to-face and shorter periodicity and not simply adding retention strategies or financial incentives favoured participation. Professionals' main challenges entailed resources, funding and, European societal changes related to communication and geopolitical environment. CONCLUSION: Retention would benefit from tailoring inclusive strategies throughout the cohorts' life cycle and consistent promotion of reciprocal altruistic research goals. Investing in regular interaction, flexibility in procedures, participant involvement and return of results can help mitigate attrition as well as considering mothers as main facilitators to participating children and impaired adults.

Survey of data collection methods and retention strategies in European birth cohorts of children and adults born very preterm.

BACKGROUND: The long-term follow-up of very preterm and very low birthweight cohorts contributes to valuable evidence to understand life-course outcomes in these vulnerable populations. However, attrition is a major challenge in long-term outcome studies. Examining the techniques used by existing cohorts may help to reveal practices that enhance willingness to continue participation over time. OBJECTIVES: To evaluate the effect of data collection methods and retention strategies on overall retention in European birth cohorts of individuals born very preterm and very low birthweight. METHODS: A survey of European cohorts integrated in the RECAP-preterm Consortium provided data on study characteristics, retention at the most recent follow-up, data collection methods and retention strategies. Cohorts were classified according to participants' age at most recent follow-up as child (<18) or adult cohorts (≥18 years old). RESULTS: Data were obtained for 17 (81%) cohorts (7 adult and 10 child) in 12 countries. Considering the baseline, at the most recent follow-up, overall retention ranged from 10% to 99%. Child cohorts presented higher median retention (68% versus 38% or 52% for adult cohorts with ≤5 or >5 follow-ups) and used relatively more retention strategies. For contact and invitation, cohorts mostly resorted to invitation letters, and to face-to-face interviews for assessments. Study duration was negatively correlated with retention and positively associated with the number of follow-up evaluation. We identified 109 retention strategies, with a median of 6 per cohort; bond-building (n = 41; 38%) was the most utilised, followed by barrier-reduction (n = 36; 33%) and reminders (n = 32; 29%). Retention was not influenced by category or number of strategies. CONCLUSIONS: Regular contact with cohort participants favour retention whilst neither the number nor the categories of retention strategies used seemed to have an influence, suggesting that tailored strategies focussed on participants at higher risk of dropout might be a more effective approach.

Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis.

Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis represents a risk to human health. This study aimed to perform the genotypic characterization of M. bovis isolated from bovines diagnosed as tuberculosis from dairy herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for microbiological culture, and colonies compatible with Mycobacterium spp. were obtained in at least one culture from 17/30 granulomas. All isolates were confirmed to be M. bovis by spoligotyping and 24loci MIRU-VNTR typing. While spoligotyping characterized the isolates as SB0121, SB0295, SB0852, SB0120, and an unclassified genotype, 24loci MIRU-VNTR rendered two clusters of two isolates each and 13 unique profiles. Loci ETR-A showed higher discriminatory power, and loci (ETR-B, ETR-C, MIRU16, MIRU27, and QUB26) showed moderate allelic diversity. This is the first study on the genetic variability of the infectious agent cause of bovine TB in Pernambuco and demonstrates variability of strains in the state. Thus, it corroborates the importance of this microorganism as agent of bovine tuberculosis and its zoonotic potential, this epidemiological tool being a determinant in the rigor of the sanitary practices of disease control in dairy herds.

Burnout in gastrenterologists: a national-level analysis.

INTRODUCTION: The term burn-out or burnout, means 'burn to exhaustion', this term was first used by Herbert Freundenberg. According to Freundenberg, burnout is a 'state of physical and mental exhaustion conditioned by the individual's professional activity'. That is manifested through a set of signs and symptoms associated with physical and emotional breakdown that result from total energy exhaustion and the lack of resources or forces to 'help' others. OBJECTIVES: Assess the occurrence of burnout in Portuguese gastroenterologists and understand how certain sociodemographic and professional variables might be associated with burnout manifestations. MATERIAL AND METHODS: Observational descriptive, prospective and multicenter study, with analyzed data related to a sample of 52 national gastroenterologists, during the time between 5 February 2019 and 13 April 2019. An estimate of the burnout levels was carried out by the Copenhagen Burnout Inventory (CBI) questionnaire validated for the Portuguese population - CBI-PT. Possible interconnection between sociodemographic and socio-professional variables were analyzed. RESULTS: More than half of the gastroenterologists surveyed did not experience burnout in all subscales of the CBI. On a personal level, there were 13 gastroenterologists with moderate burnout and 11 with high burnout. Regarding the work extent, 18 gastroenterologists with moderate burnout and 7 with high burnout were identified. When considering patient level, it was possible to determine 21 gastroenterologists with moderate burnout and 3 with high burnout. There were no cases of severe burnout. Physicians who developed burnout were significantly younger (p < .001), had less service time (p = .007) and less leisure time (p = .024). Doctors in internship had a higher prevalence of burnout, which might be significantly related to a higher level of professional stress (p = .016). The performance of certain examinations (varicose ligation and endoscopic capsule), working during the weekend and the involvement in medico-legal issues had revealed an association with a higher prevalence of burnout. DISCUSSION AND CONCLUSION: A higher prevalence of burnout was identified in young gastroenterologists, doctors in the internship, those performing high amounts of techniques (upper gastrointestinal endoscopy and colonoscopy), as well as doctors working during the weekend or involvement in legal-medical issues. Therefore, it is possible to conclude that if these young doctors do not acquire tools that will allow them to deal with this problem, it is expected that this pathology will reach critical levels within gastroenterologists, and consequently have a negative impact on healthcare.

Fluorescence Spectroscopy of Porphyrins and Phthalocyanines: Some Insights into Supramolecular Self-Assembly, Microencapsulation, and Imaging Microscopy.

The molecular interactions of anionic tetrasulfonate phenyl porphyrin (TPPS) with poly(amido amine) (PAMAM) dendrimers of generation 2.0 and 4.0 (G2 and G4, respectively) forming H- or J-aggregates, as well as with human and bovine serum albumin proteins (HSA and BSA), were reviewed in the context of self-assembly molecular complementarity. The spectroscopic studies were extended to the association of aluminum phthtalocyanine (AlPCS4) detected with a PAMAM G4 dendrimer with fluorescence studies in both steady state and dynamic state, as well as due to the fluorescence quenching associated to electron-transfer with a distribution of lifetimes. The functionalization of TPPS with peripheral substituents enables the assignment of spontaneous pH-induced aggregates with different and well-defined morphologies. Other work reported in the literature, in particular with soft self-assembly materials, fall in the same area with particular interest for the environment. The microencapsulation of TPPS studies into polyelectrolyte capsules was developed quite recently and aroused much interest, which is well supported and complemented by the extensive data reported on the Imaging Microscopy section of the Luminescence of Porphyrins and Phthalocyanines included in the present review.

Brazilian Portuguese version of the Mediterranean diet scale: Translation procedures and measurement properties.

BACKGROUND AND AIM: The Mediterranean Diet Scale (MDS) is a questionnaire with characteristics which can contribute to the multidimensional assessment of patients with diabetes mellitus (DM) by the multidisciplinary team, as well as to evaluate the effect of specific educational and nutritional interventions. The aim of this study was to translate and perform a cross-cultural adaptation of a Canadian MDS and analyze the measurement properties of the Brazilian Portuguese version of the Mediterranean Diet Scale (MDS-Brazil) in individuals with DM in Brazil. METHODS: This was a cross-sectional study. The analyzed measurement properties were the internal consistency, floor and ceiling effects, reproducibility, and construct validity. The inclusion criteria were diagnosis of DM type 1 or type 2, literate, outpatients, and without eating restrictions. RESULTS: Of the 160 volunteers included in this study, 30 participated in pretesting, and another 130 (57.7% women, 74.6% DM type 2, 56.55 ± 14.88 years) agreed to participate in the evaluation stage of the measurement properties. All items were cross-culturally adapted. A factor analysis (KMO = 0.555 and X² = 137.22; p < 0.001) extracted five factors, with no floor or ceiling effects, Cronbach's alpha = 0.42, and reproducibility ICC = 0.75. CONCLUSIONS: The cross-cultural adaptation was performed maintaining the equivalences. The MDS-Brazil measurement properties showed substantial reproducibility, low internal consistency, and fair correlations of construct validity in patients with DM.