[Dynamics of exogenous progesterone in the blood of pigs stressed with polychlorobiphenyls (PCB)]. / Dynamika exogénneho progesterónu v krvi prasiat zatazených polychlórovanými bifenylmi (PCB).

For a period of 70 days, young gilts were given rations containing a commercial mixture of polychlorinated biphenyls (PCB), produced in Czechoslovakia (Delor 105). One group of animals (A) was given feed containing 10 mg chlorobiphenyls per kg, the other group (B) was given feed containing 50 mg chlorobiphenyls per kg; on the whole, each animal of group A got 1 g of PCB mixture and each animal of group B 5 g. In about the mid-time of the experiment and before its termination the animals were given an i. m. injection of progesterone; then the animals were studied for the changes in the concentration of blood plasma progesterone. Statistically significant (P less than 0.05) and highly significant (P less than 0.01) differences in the values of the biological half-life of progesterone were found between the group of PCB-treated animals and the control group, and between groups A and B.

[The effect of polychlorinated biphenyls (PCB) on liver aniline hydroxylase activity and on certain metabolic parameters in the blood of pigs]. / Vplyv polychlórovaných bifenylov (PCB) na aktivitu pecenovej anilínhydroxylázy a na niektoré metabolické parametre krvi prasiat.

For 70 days two groups of 10-week gilts were given feed supplemented with PCB commercial mixture of Czechoslovak make (Delor 105), at the doses of 10 and 50 mg. .kg-1 of feed. Over the experimental period, animals were administered 1000 mg or 5000 mg of PCB mixture. During the experimental period the levels of glucose, cholesterol, urea, total protein, vitamin A, calcium, inorganic phosphorus and the activities of AF, ALT and AST enzymes were determined twice. At the end of this experiment the aniline hydroxylase activity in microsomal fractions of liver was determined and pronounced induction effect of PCB on the enzymatic system was observed. Out of the studied metabolic parameters of blood plasma, the decrease in vitamin A level was significant.

[Activity and variability of aminopeptidases and gamma-glutamyl transpeptidase of the cutaneous mucous membrane in the cow's rumen]. / Aktivita a variabilita aminopeptidáz a gamma-glutamyltranspeptidázy kutánnej sliznice bachora kravy

The authors studied the activity, chromatographic and electrophoretic distribution of leucylarylamidase, cystinamino peptidase, and gamma-glutamyl transpeptidase of the cytoplasmatic fraction of cow's rumen mucous membrane homogenate. The results of comparative analysis draw attention to the existence of a certain number of the molecular forms of the enzymes studied, to the activation of leucylarylamidase by Co2+, cystinamino peptidase by Co2+ and Ca2+. The possible functions of the amino peptidases and transpeptidase of glutamic acid in the metabolism of nitrogen compounds of the type of peptides, amino acids, and their amides, are discussed in the study.