RESUMO
Aspermatogenic seminiferous tubules were obtained from adult Wistar rats treated with Busulfan at the 20th day of foetel life. The isolated tubules converted 14C testosterone (T) into androstenedione (delta4), 3,5%) 5 alpha-dihydrotestosterone (DHT), (1%) ANd 5 alpha-androstane-3 beta, 17 beta-diol at unit gravity and incubated with 14C-T. Only delta was produced (2%). Using a similar technique the same result was obtained when pure preparation of round spermatids and primary spermatocytes from ram testis were incubated with 14C-T. From these experiments, we conclude that 5 alpha-reductase activity is present only in Sertoli cells.
Assuntos
Túbulos Seminíferos/metabolismo , Testículo/metabolismo , Testosterona/metabolismo , Animais , Células Cultivadas , Masculino , Ratos , OvinosRESUMO
Aspermatogenic seminiferous tubules were incubated in presence of labelled testosterone. This steroid is converted into androstenedione and 5 alpha-dihydro-testosterone. This finding favours the hypothesis which gives the Sertoli cell an important role in germ cell maturation.