Effect of protective colloids on the induction of polymorphic changes in indomethacin agglomerates after solvent evaporation from o/w emulsions.

Indomethacin (IMC) agglomerates were prepared by the solvent evaporation process from o/w emulsions containing different protective colloids in the external aqueous solution. The types of protective colloids inducing the polymorphic transformation of IMC in the agglomerates without wall material were investigated. The composition and its polymorphs were evaluated from the X-ray diffraction patterns, IR spectra and DSC thermograms. The results indicate that when pectin, beta-cyclodextrin, sodium alginate or sodium dodecyl supphase acted as a protective colloid, the respective IMC agglomerates consisted only of the alpha form of IMC. When gelatin or hydroxypropyl methylcellulose was used as a protective colloid, the amorphous, alpha and gamma forms as well as methylene chloride solvates of IMC were found in the IMC agglomerates. There was only methylene chloride solvate of IMC with a small amount of amorphous form in the IMC agglomerates prepared from albumin as a protective colloid, while IMC agglomerates prepared from methylcellulose, polyvinyl alcohol or biosoluble polymer consisted of the mixture of amorphous and alpha forms, and methylene chloride solvate of IMC. When polyvinyl pyrrolidone was applied to act as a protective colloid, the mixture of methylene chloride solvate and gamma form of IMC with less quantity of amorphous form was found in its IMC agglomerates. This strongly suggests that the composition of IMC agglomerates prepared from the solvent evaporation process was significantly influenced by the type of protective colloids used.

Experimental pyeloureterectomy and calyceal neck transection with autotransplantation and bladder advancement.

OBJECTIVE: To determine whether Dexon mesh, closely applied to the kidney, provides purchase for sutures to permit bladder/parenchymal apposition on autotransplantation and that, if this line of apposition were some distance from but surrounding renal papillae, urothelium would proliferate to cover exposed parenchyma to form a widely patent lumen; this should facilitate removal of the whole of an upper tract collecting system, retaining renal parenchyma alone. MATERIALS AND METHODS: To test this possibility and explore the practicability of the concept, nine dogs underwent bilateral nephrectomy followed by unilateral autotransplantation: the other kidney was discarded. Because the canine renal pelvis is intrarenal, the ureter was stretched maximally before passing fine scissors into the renal hilum to transect the collecting system as close to the kidney as possible in six of the nine dogs. In the remaining three dogs, partial nephrectomy was performed with division of the calyceal necks under vision. Thinned bladder wall was sutured to Dexon mesh some distance from the collecting tubules; omentum was applied to the suture line. RESULTS: Three dogs were killed prematurely at < 2 weeks because of perioperative complications. Four were killed at 2, 4, 5 and 8 weeks and two at 12 months. Dexon mesh proved to be an effective anchoring fabric, providing close apposition of bladder wall and parenchyma. There was no adhesion of the kidney to peritoneal contents. Urothelial proliferation to cover exposed parenchyma occurred early and by 12 months, a thin stroma was interposed between parenchyma and epithelium. The kidney was preserved in all but one removed electively, this dog having both cystitis and pyelonephritis at 12 months. CONCLUSIONS: This study showed that autotransplantation of a kidney after removal of its collecting system and advancement of thinned bladder wall to renal parenchyma is practicable, with regenerated urothelium bridging the deficiency by covering exposed parenchyma, to create a widely patent lumen.