RESUMO
Two distinctly different substitution principles are commonly used in haemophilia: treatment at bleeding episodes only referred to as on-demand treatment, and prophylactic factor administration. The aim of the cross-sectional study which was undertaken in young patients suffering severe haemophilia A was to challenge our hypothesis that on-demand treatment is inferior to prophylactic substitution in prevention of chronic joint disease at young age. The method involved an investigation of 40 patients from Russia (n = 27) and Denmark (n = 13) born between 1975 and 1990 with no history of inhibitors; Russian patients had exclusively received factor VIII on demand, while Danish patients were managed with prophylactic treatment during a mean period of 16 years since median age of 5 years. The study endpoints were clinical joint scores, Quality of Life scores and functional independence scores. Matched by identical age (±1 year) 13 Danish and 13 Russian patients were compared, while 14 age similar Russian patients served as controls. Demographic data among all groups were quite comparable. The results are that Russian patients presented with clinical joint scores at 27 ± 8.5 (mean ± SD) while matched Danish counterparts scored 3.8 ± 5.3 (mean ± SD), differences being highly significant. The number of joint bleeds in recent 5 years were 199.5 ± 135 (mean ± SD) vs. 8.1 ± 8.7 (mean ± SD). Likewise, Quality of Life and functional independence scores were significantly higher in patients on prophylaxis as compared to on-demand treatment. In conclusion, the study outcomes confirmed our hypothesis. Longer term prophylactic factor administration during childhood and adolescence prevents joint destruction.
Assuntos
Fator VIII/uso terapêutico , Hemofilia A/tratamento farmacológico , Pré-Medicação , Adolescente , Adulto , Fatores Etários , Coagulação Sanguínea , Estudos Transversais , Dinamarca , Fator VIII/administração & dosagem , Hemofilia A/sangue , Hemofilia A/complicações , Humanos , Qualidade de Vida , Federação Russa , Índice de Gravidade de Doença , Resultado do Tratamento , Adulto JovemRESUMO
It is now known that the electric charge of a surface cellular membrane is a rather characteristic value for each type of a cell. Cellular electrophoresis may be used to study the electrokinetic properties of red blood cells in each specific case, thereby obtaining indirect information on the state of a surface membrane as the electrophoretic mobility (EPM) rate is directly proportional to the value of a cellular electric charge. The study was undertaken to compare the EPM rates of peripheral red blood cells from donors, by employing two different preservatives: EDTCHA and sodium citrate. No statistically significant differences were found in the groups under study and these parameters were comparable.
Assuntos
Membrana Eritrocítica/química , Conservantes Farmacêuticos/química , Eletroforese/métodos , Humanos , Preservação Biológica/métodosAssuntos
Anemia Aplástica/diagnóstico por imagem , Doenças da Medula Óssea/diagnóstico por imagem , Medula Óssea/diagnóstico por imagem , Fígado/diagnóstico por imagem , Pancitopenia/diagnóstico por imagem , Baço/diagnóstico por imagem , Adolescente , Adulto , Doença Crônica , Coloides , Hepatite Crônica/diagnóstico por imagem , Humanos , Pessoa de Meia-Idade , Cintilografia , TecnécioRESUMO
Activity of adenosine deaminase (EC 3.5.4.4) was studied in thrombocytes of donors and patients with various hematological diseases. The enzymatic activity was decreased in acute leukemia, chronic myeloleukemia, chronic leukemia and blast transformation myeloma, microspherocytic and hypoplastic anemias. Variable level of the activity was observed in chronic lympholeukemia and non-Hodgkin disease. In all the diseases studied functions of thrombocytes were altered after treatment with various aggregating agents (ADP, thrombin, collagen, adrenaline, ristomycin).
Assuntos
Adenosina Desaminase/sangue , Plaquetas/enzimologia , Doenças Hematológicas/enzimologia , Nucleosídeo Desaminases/sangue , Anemia/sangue , Anemia/enzimologia , Doenças Hematológicas/sangue , Humanos , Transtornos Linfoproliferativos/sangue , Transtornos Linfoproliferativos/enzimologia , Transtornos Mieloproliferativos/sangue , Transtornos Mieloproliferativos/enzimologia , Agregação PlaquetáriaRESUMO
Activity of purine nucleoside phosphorylase (EC 2.4.2.1) was estimated in thrombocytes of donors and of patients with various hematological diseases. The enzymatic activity was decreased in chronic lymphoid leukosis chronic myeloleukemia, chronic myeloleukemia and blast transformation, acute lymphoblast and myeloblast leukemia, myeloma. Dissimilar level of purine nucleoside phosphorylase activity was found in non-Hodgkin disease. The activity was only slightly increased in Waldenström's macroglobulinemia; it was similar to normal level in hypoplastic and autoimmune hemolytic anemias. A decrease in the enzymatic activity was observed in thrombocytes of the patients with hereditary forms of hemorrhagic diatheses (hemophilia A, Willebrand disease). Normal level of the enzymatic activity was noted in Glanzmann thrombasthenia.
Assuntos
Plaquetas/enzimologia , Doenças Hematológicas/enzimologia , Pentosiltransferases/sangue , Purina-Núcleosídeo Fosforilase/sangue , Doadores de Sangue , Doenças Hematológicas/genética , Humanos , Leucemia/enzimologiaAssuntos
Doenças Hematológicas/diagnóstico , Hepatopatias/diagnóstico , Adolescente , Adulto , Anemia/diagnóstico , Anemia Hemolítica/diagnóstico , Anticorpos/análise , Doenças Autoimunes/diagnóstico , Transplante de Medula Óssea , Criança , Feminino , Doenças Hematológicas/fisiopatologia , Doenças Hematológicas/terapia , Hematopoese , Hepatite Viral Humana/diagnóstico , Humanos , Fígado/imunologia , Cirrose Hepática Alcoólica/diagnóstico , Hepatopatias/fisiopatologia , Hepatopatias/terapia , Masculino , Pancitopenia/diagnóstico , Esplenomegalia/diagnóstico , Macroglobulinemia de Waldenstrom/diagnósticoAssuntos
Linfoma/tratamento farmacológico , Antineoplásicos/administração & dosagem , Ciclofosfamida/administração & dosagem , Avaliação de Medicamentos , Quimioterapia Combinada , Humanos , Compostos de Mostarda Nitrogenada/administração & dosagem , Prednisolona/administração & dosagem , Fatores de TempoAssuntos
Membranas Artificiais , Fenotiazinas , Eletroquímica , Lipossomos , Propriedades de SuperfícieRESUMO
An inhibitor of adenase, which is absent in leukemic patients, was studied in thrombocytes of healthy persons. The inhibitor, localized in the granular fraction, was isolated by ultracentrifugation in sucrose gradient.
Assuntos
Aminoidrolases/antagonistas & inibidores , Plaquetas/enzimologia , Humanos , Leucemia Linfoide/sangue , Leucemia Linfoide/enzimologiaRESUMO
An attempt is made to ascertain the mechanism of increased fibrinolytic activity in patients suffering from acute leukemia. Investigations confirmed a possibility of both the primary and the secondary character of hyperfibrinolysis which required the choice of rational therapeutic tactics in each individual case.