Dynamic shifts of functional diversity through climate-Resilient strategies and farmland restoration in a mountain protected area.

Land-use land-cover (LULC) change contributes to major ecological impacts, particularly in areas undergoing land abandonment, inducing modifications on habitat structure and species distributions. Alternative land-use policies are potential solutions to alleviate the negative impacts of contemporary tendencies of LULC change on biodiversity. This work analyzes these tendencies in the Montesinho Natural Park (Portugal), an area representative of European abandoned mountain rural areas. We built ecological niche models for 226 species of vertebrates (amphibians, reptiles, birds, and mammals) and vascular plants, using a consensus modelling approach available in the R package 'biomod2'. We projected the models to contemporary (2018) and future (2050) LULC scenarios, under four scenarios aiming to secure relevant ecosystem services and biodiversity conservation for 2050: an afforestation and a rewilding scenario, focused on climate-smart management strategies, and a farmland and an agroforestry recovery scenario, based on re-establishing human traditional activities. We quantified the influences of these scenarios on biodiversity through species habitat suitability changes for 2018-2050. We analyzed how these management strategies could influence indices of functional diversity (functional richness, functional evenness and functional dispersion) within the park. Habitat suitability changes revealed complementary patterns among scenarios. Afforestation and rewilding scenarios benefited more species adapted to habitats with low human influence, such as forests and open woodlands. The highest functional richness and dispersion was predicted for rewilding scenarios, which could improve landscape restoration and provide opportunities for the expansion and recolonization of forest areas by native species. The recovery of traditional farming and agroforestry activities results in the lowest values of functional richness, but these strategies contribute to complex landscape matrices with diversified habitats and resources. Moreover, this strategy could offer opportunities for fire suppression and increase landscape fire resistance. An integrative approach reconciling rewilding initiatives with the recovery of extensive agricultural and agroforestry activities is potentially an harmonious strategy for supporting the provision of ecosystem services while securing biodiversity conservation and functional diversity within the natural park.

Diagnostic capability of artificial intelligence tools for detecting and classifying odontogenic cysts and tumors: a systematic review and meta-analysis.

OBJECTIVE: To evaluate the diagnostic capability of artificial intelligence (AI) for detecting and classifying odontogenic cysts and tumors, with special emphasis on odontogenic keratocyst (OKC) and ameloblastoma. STUDY DESIGN: Nine electronic databases and the gray literature were examined. Human-based studies using AI algorithms to detect or classify odontogenic cysts and tumors by using panoramic radiographs or CBCT were included. Diagnostic tests were evaluated, and a meta-analysis was performed for classifying OKCs and ameloblastomas. Heterogeneity, risk of bias, and certainty of evidence were evaluated. RESULTS: Twelve studies concluded that AI is a promising tool for the detection and/or classification of lesions, producing high diagnostic test values. Three articles assessed the sensitivity of convolutional neural networks in classifying similar lesions using panoramic radiographs, specifically OKC and ameloblastoma. The accuracy was 0.893 (95% CI 0.832-0.954). AI applied to cone beam computed tomography produced superior accuracy based on only 4 studies. The results revealed heterogeneity in the models used, variations in imaging examinations, and discrepancies in the presentation of metrics. CONCLUSION: AI tools exhibited a relatively high level of accuracy in detecting and classifying OKC and ameloblastoma. Panoramic radiography appears to be an accurate method for AI-based classification of these lesions, albeit with a low level of certainty. The accuracy of CBCT model data appears to be high and promising, although with limited available data.

Nutritional value and kinetics of in vitro fermentation of spineless cactus of the genus Nopalea in different phenological phases.

The objective was to evaluate the chemical composition and in vitro fermentation of spineless cactus of the genus Nopalea, F-21 (Nopalea cochenillifera Dyck), IPA-Sertânia (Nopalea cochenillifera Dyck) and Miúda (Nopalea cochenillifera Salm Dyck), in different phenophases. There was no effect (P < 0.05) of the phenological phases of spineless cactus on DM, ash, OM, EE, and CP. Varieties F-21 and Miúda presented higher values of DM and OM, whereas the CP was higher for IPA-Sertânia. The contents of NDF, ADF, and ADL, as well as the fractions of carbohydrates B2 and C were higher in the mature stage, irrespective of the variety. The Miúda variety showed higher levels of NFC and fractions A + B1 and the lower levels of pectin compared to the F-21 and IPA-Sertânia varieties, but not differ of TC to F-21. The volume of gas produced via the degradation of NFC was higher for young phenological phases. The young and intermediate stages showed a higher in vitro digestibility of DM. Based on the results, varieties IPA-Sertânia and Miúda have a high potential for use in animal feed because of their high nutritional quality. Mature cladodes showed a higher fibrous fraction and lower digestibility in all varieties.

Biodiversity dataset and atlas of the special area of conservation Montesinho/Nogueira, Portugal.

Background: The special area of conservation Montesinho/Nogueira (SAC-MN) is a key area for biodiversity conservation in the Iberian Peninsula. Covering an area of approximately 1081 km² in the northeast of Portugal mainland, the SAC-MN is home to a wide range of species, including several endemic and endangered species and priority habitats. Despite its ecological significance and importance for conservation, there is a lack of publicly available biodiversity data, which urges the need to create a comprehensive and up-to-date biodiversity dataset for the SAC-MN. New information: To bridge the knowledge gap on biodiversity in SAC-MN, we undertook a thorough data collection process, including species occurrence records and conservation status information at regional (Portugal) and European levels, from multiple sources. We collected and compiled this information for five major taxonomic groups (amphibians, birds, flora - vascular plants, mammals and reptiles) in SAC-MN, resulting in a total of 31,871 records with 1,312 documented species. In addition, we developed an easy-to-navigate web-based geographic information system (Web GIS). In this article, we present an in-depth report on the process of compiling and preparing data, as well as the development and design of our Web GIS to increase awareness and enhance understanding of the importance of preserving biodiversity in SAC-MN.

Impact of feeding native Caatinga pasture on the rumen histomorphometry of sheep raised in semi-extensive management.

In the northeastern region of Brazil, sheep and goat farming, encompassing around 20 million animals, is predominantly a subsistence activity. Forage quality plays a crucial role in animal productivity, posing a complex interplay between plant and animal aspects. The Caatinga biome, vital for livestock in the region, serves as a significant source for animal diet through pastures. This study aimed to conduct a histomorphometric evaluation of sheep rumens in a semi-extensive system, comparing those feeding on native Caatinga pastures to those on cultivated pastures. Histological processing followed standard protocols, with morphometry focusing on six viable rumen papillae and the submucosa and muscular layer thickness. Statistical correlation analysis revealed morphological differences in papillae across various rumen regions. Morphometric data indicated no significant difference in papillae area between the groups, with average values in Group A surpassing those in Group B, except for width. This study establishes a morphological and morphometric pattern for rumen regions linked to diet types-native or cultivated. The findings not only enhance understanding of the dietary foundation in the Caatinga's extensive system, but also contribute valuable insights for formulating nutritional strategies to enhance sheep production in the region. This research sheds light on the intricacies of forage-based animal nutrition, particularly in semi-extensive systems, offering a foundation for future studies and practices to optimise livestock management in the northeastern Brazilian context.

Time-series analyses of land surface temperature changes with Google Earth Engine in a mountainous region.

Studying changes in temperature is fundamental for understanding its interactions with the environment and biodiversity. However, studies in mountainous areas are few, due to their complex formation and the difficulty of obtaining local data. We analysed changes in temperature over time in Montesinho Natural Park (MNP) (Bragança, Portugal), an important conservation area due to its high level of biodiversity. Specifically, we aimed to analyse: i) whether temperature increased in MNP over time, ii) what environmental factors influence the Land Surface Temperature (LST), and iii) whether vegetation is related to changes in temperature. We used annual summer and winter mean data acquired from the Moderate-Resolution Imaging Spectroradiometer (MODIS) datasets/products (e.g. LST, gathered at four different times: 11am, 1pm, 10pm and 2am, Enhance vegetation index - EVI, and Evapotranspiration - ET), available on the cloud-based platform Google Earth Engine between 2003 and 2021). We analysed the dynamics of the temporal trend patterns between the LST and local thermal data (from a weather station) by correlations; the trends in LST over time with the Mann-Kendall trend test; and the stability of hot spots and cold spots of LST with Local Statistics of Spatial Association (LISA) tests. The temporal trend patterns between LST and Air Temperature (Tair) data were very similar (ρ > 0.7). The temperature in the MNP remained stable over time during summer but increased during winter nights. The biophysical indices were strongly correlated with the summer LST at 11am and 1pm. The LISA results identified hot and cold zones that remained stable over time. The remote-sensed data proved to be efficient in measuring changes in temperature over time.

Bradykinin promotes murine melanoma cell migration and invasion through endogenous production of superoxide and nitric oxide.

Spatial confinement and temporal regulation of signaling by nitric oxide (NO) and reactive oxygen species (ROS) occurs in cancer cells. Signaling mediated by NO and ROS was investigated in two sub clones of the murine melanoma B16F10-Nex2 cell line, Nex10C and Nex8H treated or not with bradykinin (BK). The sub clone Nex10C, similar to primary site cells, has a low capacity for colonizing the lungs, whereas the sub clone Nex8H, similar to metastatic cells, corresponds to a highly invasive melanoma. BK-treated Nex10C cells exhibited a transient increase in NO and an inhibition in basal O2- levels. Inhibition of endogenous NO production by l-NAME resulted in detectable levels of O2-. l-NAME promoted Rac1 activation and enhanced Rac1-PI3K association. l-NAME in the absence of BK resulted in Nex10C cell migration and invasion, suggesting that NO is a negative regulator of O2- mediated cell migration and cell invasion. BK-treated Nex8H cells sustained endogenous NO production through the activation of NOS3. NO activated Rac1 and promoted Rac1-PI3K association. NO stimulated cell migration and cell invasion through a signaling axis involving Ras, Rac1 and PI3K. In conclusion, a role for O2- and NO as positive regulators of Rac1-PI3K signaling associated with cell migration and cell invasion is proposed respectively for Nex10C and Nex8H murine melanoma cells.

Social Vulnerability of Brazilian Metropolitan Schools and Teachers' Absence from Work Due to Vocal and Psychological Symptoms: A Multilevel Analysis.

Teachers' voices and psychological symptoms are the main reasons for absence from work. The objectives of this study were: (i) to spatially represent, through a webGIS, the standardized rates of teachers' absences due to voice (outcome 1) and psychological symptoms (outcome 2) in each Brazilian Federative Unit (FU = 26 states plus Federal District) and (ii) to analyze the relationship between each national outcome rate and the Social Vulnerability Index (SVI) of the municipality where urban schools are located, adjusted for sex, age, and working conditions. This cross-sectional study comprised 4979 randomly sampled teachers working in basic education urban schools, of which 83.3% are women. The national absence rates were 17.25% for voice symptoms and 14.93% for psychological symptoms. The rates, SVI, and school locations in the 27 FUs are dynamically visualized in webGIS. The multilevel multivariate logistic regression model showed a positive association between voice outcome and high/very high SVI (OR = 1.05 [1.03; 1.07]), whereas psychological symptoms were negatively associated with high/very high SVI (OR = 0.86 [0.85 0.88]) and positively associated with intermediate SVI (OR = 1.15 [1.13; 1.16]), in contrast with low/very low SVI. Being a woman (voice: OR = 1.36 [1.35; 1.38]; psychological: 1.22 [1.21; 1.24]) and working in schools with various precarious conditions (17 variables) increased the odds of being absent due to voice and psychological symptoms. The results confirm the need for investments to improve working conditions in schools.

Homo zappiens: nômades digitais e as subjetividades no âmbito da autogestão lazer-trabalho / Homo zappiens: digital nomads and subjectivities within leisure-work self-management

Este estudo, de natureza qualitativa, teve por objetivo investigar os conteúdos informacionais disseminados no site Movimento Nômades Digitais, buscando compreender os processos de autogestão de lazer-trabalho nesse estilo de viver. A pesquisa do tipo exploratória foi pautada em análise documental e estudo de caso. Os resultados apontam alguns paradoxos no equilíbrio entre as formas de concepção da autogestão do trabalho e as possíveis ressonâncias na autogestão do lazer. Essas mudanças no estilo do trabalho podem não representar, igualmente, mais flexibilidade de tempo e possibilidades de vivências de experiências significativas no lazer, sobretudo, pelos aspectos econômicos, políticos, culturais e organizacionais envolvidos. A autogestão do lazer parece não poder ser diretamente associada à autogestão do trabalho dos nômades digitais, já que outros elementos entram em cena, para além da opção pessoal em si.

This qualitative study aimed to investigate the informational content disseminated on the Movimento Nômades Digitais website, seeking to understand the leisure-work self-management processes in this lifestyle. Exploratory research was based on document analysis and case study. Results point to some paradoxes in the balance between the forms of conception of work self-management and the possible resonances in leisure self-management. These changes in the style of work may not represent, equally, more flexibility of time and possibilities of living meaningful experiences in leisure, due to the economic, political, cultural and organizational aspects involved. The self-management of leisure does not seem to be directly associated with the self-management of work by digital nomads, since other elements come into play, in addition to the personal option itself.

Prevalence of mandibular asymmetry in different skeletal sagittal patterns.

OBJECTIVES: To analyze the prevalence of mandibular asymmetry in skeletal sagittal malocclusions. MATERIALS AND METHODS: PubMed/MEDLINE, EMBASE, LILACS, Web of Science, Scopus, LIVIVO and gray literature (OpenGrey, ProQuest, and Google Scholar) were electronically searched. Two independent investigators selected the eligible studies, and assessed risk of bias and certainty of evidence (GRADE). One reviewer independently extracted the data and the second reviewer checked this information. Any disagreement between the reviewers in each phase was resolved by discussion between them and/or involved a third reviewer for final decision. RESULTS: Electronic search identified 5,132 studies, and 5 observational studies were included. Risk of bias was low in two studies, moderate in one, and high in two. The studies showed high heterogeneity. Mandibular asymmetry ranged from 17.43% to 72.95% in overall samples. Horizontal chin deviation showed a prevalence of 17.66% to 55.6% asymmetry in Class I malocclusions, and 68.98% in vertical asymmetry index. In Class II patients, prevalence of mandibular asymmetry varied from 10% to 25.5% in horizontal chin deviation, and 71.7% in vertical asymmetry index. The Class III sample showed a prevalence of mandibular asymmetry ranging from 22.93% to 78% in horizontal chin deviation and 80.4% in vertical asymmetry index. Patients seeking orthodontic or orthognathic surgery treatment showed greater prevalence of mandibular asymmetry. CONCLUSIONS: Skeletal Class III malocclusion showed the greatest prevalence of mandibular asymmetry. Mandibular vertical asymmetry showed a marked prevalence in all malocclusions. However, conclusions should be interpreted with caution due to use of convenience samples and low-quality study outcomes.

Malignant tumours mimicking periapical lesions: A report of three cases and literature review.

Apical periodontitis shows radiographic signs such as widening of the periodontal ligament and periapical radiolucency, which differ in extent depending on the stage of the lesion. However, other lesions can be associated with or coincidental to the apical region, representing developmental lesions and benign or malignant tumours. This article describes three cases of malignant tumours, a central mucoepidermoid carcinoma (CMEC), a chondroblast osteosarcoma and an osteosarcoma of the jaw (OSJ) that presented as periapical lesions. Endodontists must be aware of unsuccessful treatment, persistent pain, signs of paraesthesia, a rapid growth rate and delayed response to therapy associated with atypical features. Complementary examinations, such as biopsy and computed tomography, can allow the early diagnosis of malignant tumours, leading to a better prognosis and thus increased survival rates and improvement in quality of life.

Patterns of regulatory behavior in the still-face paradigm at 3 months: A comparison of Brazilian and Portuguese infants.

Three infant regulatory behavior patterns have been identified during the Face-to-Face Still-Face paradigm (FFSF) in prior research samples: a Social-Positive Oriented pattern (i.e., infants exhibit predominantly positive social engagement), a Distressed-Inconsolable pattern (i.e., infants display conspicuous negative affect that persists or increases across FFSF episodes), and a Self-Comfort Oriented pattern (e.g., infants primarily engage in self-comforting behaviors such as thumb-sucking). However, few studies have examined these patterns outside US and European countries or evaluated potential cross-country differences in these patterns. In this study, we compared the regulatory behavior patterns of 74 Brazilian and 124 Portuguese infants in the FFSF at 3 months of age, and evaluated their links to demographic and birth variables. The prevalence of the three regulatory patterns varied by country. The most frequent pattern in the Portuguese sample was the Social-Positive Oriented, followed by the Distressed-Inconsolable and the Self-Comfort Oriented. However, in the Brazilian sample, the Distressed-Inconsolable pattern was the most prevalent, followed by the Social-Positive Oriented and the Self-Comfort Oriented. Moreover, in the Brazilian sample, familial SES was higher among infants with a Social-Positive pattern whereas 1st-minute Apgar scores were lower among Portuguese infants with a Distressed-Inconsolable Oriented pattern of regulatory behavior. In each sample, Social Positive pattern of regulatory behavior was associated with maternal sensitivity, Self-Comfort Oriented pattern of regulatory behavior with maternal control, and Distressed-Inconsolable pattern with maternal unresponsivity.

Host-virus interaction and viral evasion.

With each infectious pandemic or outbreak, the medical community feels the need to revisit basic concepts of immunology to understand and overcome the difficult times brought about by these infections. Regarding viruses, they have historically been responsible for many deaths, and such a peculiarity occurs because they are known to be obligate intracellular parasites that depend upon the host's cell machinery for their replication. Successful infection with the production of essential viral components requires constant viral evolution as a strategy to manipulate the cellular environment, including host internal factors, the host's nonspecific and adaptive immune responses to viruses, the metabolic and energetic state of the infected cell, and changes in the intracellular redox environment during the viral infection cycle. Based on this knowledge, it is fundamental to develop new therapeutic strategies for controlling viral dissemination, by means of antiviral therapies, vaccines, or antioxidants, or by targeting the inhibition or activation of cell signaling pathways or metabolic pathways that are altered during infection. The rapid recovery of altered cellular homeostasis during viral infection is still a major challenge. Here, we review the strategies by which viruses evade the host's immune response and potential tools used to develop more specific antiviral therapies to cure, control, or prevent viral diseases.

Lazer e cultura digital: abordagens sobre e-leisure em periódicos do JCR / Leisure and digital culture: e-leisure approaches in JCR Journals

Os avanços das tecnologias imprimiram a necessidade de alterações dos paradigmas que envolviam os estudos do lazer (EL), instigando novos olhares sobre as vivências no e-leisure. Assim, procurou-se investigar como as abordagens acerca da temática e-leisure são apropriadas nos EL, dentro dos periódicos dessa área, cadastrados na base do Journal Citation Reports (JCR). Foram criadas as categorias de análise: Eleisure e o lazer off-line; Compreensão sobre a incorporação do e-leisure na sociedade contemporânea; O papel do e-leisure na interação social. Os dados revelam o papel do lazer digital como fomentador de valores e de aproximação de culturas. Conclui-se que o e-leisure facilita o compartilhamento de diferentes culturas, podendo proporcionar a expansão das fronteiras sociais e políticas. Entretanto, também pode gerar alguns impactos negativos, haja vista as possibilidades do uso indevido do espaço virtual.

Advances in technologies have led to the need for changes in the paradigms that involve leisure studies (LS), instigating new perspectives on e-leisure experiences. Thus, we sought to investigate how the approaches regarding e-leisure are appropriate in LS within the journals in this field and registered in the Journal Citation Reports (JCR). The analysis categories were created: E-leisure and offline leisure; Understanding e-leisure integration in contemporary society and the role of e-leisure in social interaction. The data reveal the digital leisure as an enabler for values and approximation of cultures. It is concluded that e-leisure facilitates the sharing of different cultures, providing the expansion of social and political boundaries. However, given some possibilities of misuse of the virtual space, it may also result in negative impacts.

Geospatial Analysis of Environmental Atmospheric Risk Factors in Neurodegenerative Diseases: A Systematic Review.

Despite the vast evidence on the environmental influence in neurodegenerative diseases, those considering a geospatial approach are scarce. We conducted a systematic review to identify studies concerning environmental atmospheric risk factors for neurodegenerative diseases that have used geospatial analysis/tools. PubMed, Web of Science, and Scopus were searched for all scientific studies that included a neurodegenerative disease, an environmental atmospheric factor, and a geographical analysis. Of the 34 included papers, approximately 60% were related to multiple sclerosis (MS), hence being the most studied neurodegenerative disease in the context of this study. Sun exposure (n = 13) followed by the most common exhaustion gases (n = 10 for nitrogen dioxide (NO2) and n = 5 for carbon monoxide (CO)) were the most studied atmospheric factors. Only one study used a geospatial interpolation model, although 13 studies used remote sensing data to compute atmospheric factors. In 20% of papers, we found an inverse correlation between sun exposure and multiple sclerosis. No consensus was reached in the analysis of nitrogen dioxide and Parkinson's disease, but it was related to dementia and amyotrophic lateral sclerosis. This systematic review (number CRD42020196188 in PROSPERO's database) provides an insight into the available evidence regarding the geospatial influence of environmental factors on neurodegenerative diseases.

Family SES and maternal sensitivity predict infant patterns of regulatory behavior in Brazilian dyads.

Infant's patterns of regulatory behavior contribute to infant socioemotional development and attachment. These behavioral patterns affect and are affected by the quality of mother-infant interaction. In most studies with full-term infants, the Social-Positive Oriented pattern (i.e., the infant's ability to soothe his/her emotions in the context of reciprocal and positive interactions) is the most prevalent pattern, followed by the Distressed-Inconsolable and by the Self-Comfort Oriented patterns. However, these patterns are understudied in other populations beyond the US and European countries. The current research addresses this gap by studying the regulatory behavior patterns and their association with mother-infant interactions in Brazilian dyads and evaluating the association of these regulatory patterns with demographics. Analyses were based on data collected for 40 infants (20 boys, 20 girls) and their mothers. Infants' regulatory behavior patterns were evaluated in the Face-to-Face Still-Face paradigm and mother-infant interaction was evaluated during free play at 3 months age. Notably, our findings indicate that Distressed-Inconsolable was the most prevalent pattern in this sample; followed by the Social-Positive Oriented and the Self-Comfort Oriented patterns. Furthermore, we found that maternal sensitivity and family SES (social-economic status) predicted infant patterns of regulatory behavior.

A dimensão tempo na gestão das experiências de lazer em período de pandemia da covid-19 no Brasil / The time dimension in the management of leisure experiences in the pandemic period of covid-19 in Brazil

Este estudo tem como objetivo analisar a dimensão tempo na gestão das experiências de lazer durante o distanciamento social por conta da pandemia COVID-19. A pesquisa, de natureza quali-quantitativa, do tipo descritiva, utilizou estudo de campo mediante a aplicação de um questionário on-line. A amostra foi composta de 2.278 brasileiros, acima de 18 anos. A análise dos dados foi realizada por meio de estatística descritiva e análise de conteúdo. Os resultados indicaram que a maioria dos respondentes teve tempo para as experiências de lazer, embora a maioria também tenha relatado que esse tempo diminuiu neste período. Entre os conteúdos culturais do lazer, os mais vivenciados de acordo com o tempo foram: os virtuais, os intelectuais e os manuais. Sugerem-se novos estudos que abordem esta temática, associando-os a outras dimensões do lazer.

This study aims to analyze the time dimension in the management of leisure experiences during social distancing due to the COVID-19 pandemic. The research, of a quali-quantitative nature, descriptive type, used a field study approach through the application of an online questionnaire. The sample consisted of 2,278 Brazilians, over 18 years of age. Data analysis was performed through descriptive statistics and content analysis. Results indicated that the majority of respondents had time for leisure experiences, although most of them also reported that this time decreased during this period. Among the cultural contents of leisure, the most prevalent were: virtual, intellectuals and manuals experiences. Further studies are recommended to address this theme, by associating them with other dimensions of leisure.

Programa lúdico para o âmbito corporativo: interesse e satisfação de funcionários / Program of playful interventions in the corporate field: interest and satisfaction of the enployees

Este estudo qualitativo objetivou avaliar o interesse e a satisfação de participantes de um programa lúdico para o âmbito corporativo e foi desenvolvido por meio de pesquisas bibliográfica e exploratória. A amostra contou com 25 funcionários de uma empresa, escolhida por conveniência, os quais participaram de um programa lúdico de longo prazo, visando refletir sobre problemas relacionados ao contexto do trabalho, de forma colaborativa, por meio de jogos. Os resultados da aplicação de escala do tipo Likert, analisados descritivamente pela Técnica de Análise de Conteúdo, indicam alto interesse e satisfação dos participantes frente às intervenções lúdicas realizadas durante o programa oferecido. Com base nesses resultados, torna-se relevante a ampliação das discussões sobre esta temática, no sentido de incentivar novas práticas envolvendo o lúdico e empresas.

This qualitative study aimed to analyze the interest and satisfaction of the participants of a program interventions playfulin the corporate field, developed through bibliographical and exploratory researches. Twenty-five employees, of a chosen company, took part in a long-term program inteventions playful in order to reflect upon the problems related to the working environment in a collaborative way through games. The results of the application of a Likert scale which was descriptively analyzed by Content Analysis Technique and pointed out the high participant's interest and satisfaction concerning the playful activities that were present during the offered program. Based on these results, it is relevant to broaden the discussions about this issue so as to encourage new practices involving interventions playful in companies.

Language, neurodevelopment, and behavior in Angelman syndrome: a case report. / Linguagem, neurodesenvolvimento e comportamento na Síndrome de Angelman: relato de caso.

PURPOSE: This study aimed to present findings on language, behavior, and neurodevelopment in a girl diagnosed with Angelman Syndrome, evaluated when she was three and eight years old. METHODS: The following evaluation instruments were used: Observation of Communication Behavior, Early Language Milestone (ELM) Scale, and Denver Developmental Screening Test, 2nd edition (DDST-II). RESULTS: In this case report, presence of AS phenotype signals such as wide mouth and wide-spaced teeth, tongue thrusting, strabismus, up slanting palpebral fissures, and sialorrhea are verified. Expressive and receptive deficits were verified in the language assessment, with the absence of orality and loss of comprehension with very similar performances in both evaluations. The ELM and DDST-II tests indicated severe impairment of all abilities evaluated at both three and eight years of age. Performance was quite similar in both evaluations in all areas of child development. Little progress was observed over time despite the great therapeutic and educational investment. CONCLUSION: The presence of a complex scenario such as AS demands high complexity clinical needs, a situation that is worsened due to scarcity of therapeutic resources that could minimize the harmful impacts of AS and culminate in increased quality of life for the AS population and their families.

OBJETIVO: O objetivo deste estudo é apresentar achados de linguagem, comportamento e neurodesenvolvimento de uma menina com diagnóstico da Síndrome de Angelman, avaliada aos três e aos oito anos. MÉTODO: Os instrumentos de avaliação foram Observação do Comportamento Comunicativo, Early Language Milestone Scale (ELM) e Teste de Screening de Desenvolvimento DENVER-II (TSDD-II). RESULTADOS: No caso apresentado, verifica-se a presença dos sinais fenotípicos da SA, tais como boca larga, dentes espaçados, língua protuberante, estrabismo, fissuras palpebrais ascendentes e sialorreia. Na avaliação de linguagem, foram verificados déficits expressivos e receptivos, com ausência de oralidade e prejuízos na compreensão. O TSDD-II e a ELMS indicaram grave comprometimento de todas as habilidades avaliadas aos três e aos oito anos. O desempenho encontrado, nas duas avaliações, foi muito semelhante em todas as áreas do desenvolvimento infantil. Ao longo dos anos, verificou-se pouca evolução, apesar do grande investimento terapêutico e educacional. CONCLUSÃO: A presença de um quadro complexo como a SA demanda necessidades clínicas de alta complexidade, situação agravada frente à escassez de recursos terapêuticos que possam minimizar os impactos deletérios da síndrome, culminando em comprometimento da qualidade de vida da população com a SA, bem como de suas famílias.

Nitric oxide and interactions with reactive oxygen species in the development of melanoma, breast, and colon cancer: A redox signaling perspective.

Cancer development is closely related to chronic inflammation, which is associated with identifiable markers of tumor progression, such as uncontrolled cell proliferation, angiogenesis, genomic instability, chemotherapeutic resistance, and metastases. Redox processes mediated by reactive oxygen species (ROS) and nitric oxide (NO) within the inflammatory tumor microenvironment play an essential role in directly influencing intercellular and intracellular signaling. These reactive species originating in the cancer cell or its microenvironment, mediate the epithelial-mesenchymal transition (EMT) and the mesenchymal-epithelial transition (MET). However, intracellular interactions between NO and ROS must be controlled to prevent cell death. Melanoma, breast, and colon cancer cells have developed a mechanism to survive and adapt to oxidative and nitrosative stress. The mechanism involves a spatial-temporal fine adjustment of the intracellular concentrations of NO and ROS, thereby guaranteeing the successful development of cancer cells. Physiological concentrations of NO and supra physiological concentrations of ROS are prevalent in cancer cells at the primary site. The situation reverses in cancer cells undergoing the EMT prior to being released into the blood stream. Intracellular supra physiological concentrations of NO found in circulating cancer cells endow them with anoikis resistance. When the anoikis-resistant cancer cells arrive at a metastatic site they undergo the MET. Endogenous supra physiological concentrations of ROS and physiological NO concentrations are prevalent in these cells. Understanding tumor progression from the perspective of redox signaling permits the characterization of new markers and approaches to therapy. The synthesis and use of compounds with the capacity of modifying intracellular concentrations of NO and ROS may prove effective in disrupting a redox homeostasis operative in cancer cells.