RESUMO
As the result of our investigations, newly isolated B. pertussis and B. bronchiseptica strains were studied. The results of these investigations showed that B. pertussis strains isolated under the conditions of immunoprophylaxis were characterized by sufficient stability of the main phenotypical properties which determined their pathogenicity: B. pertussis toxin, fimbrial agglutinogens and filamentous hemagglutinin. At the same time B. bronchiseptica strains isolated from animals proved to be phenotypically variable both in vivo and in the process of in vitro passage.
Assuntos
Bordetella/isolamento & purificação , Adolescente , Aglutininas/análise , Criação de Animais Domésticos , Animais , Bordetella/análise , Bordetella/patogenicidade , Bordetella pertussis/análise , Bordetella pertussis/isolamento & purificação , Bordetella pertussis/patogenicidade , Criança , Pré-Escolar , Fímbrias Bacterianas/química , Hemaglutininas/análise , Humanos , Lactente , Toxina Pertussis , Fenótipo , Especificidade da Espécie , Suínos/microbiologia , Ucrânia , Fatores de Virulência de Bordetella/análiseRESUMO
Comparative assessment of the diagnostic properties of 30 lots of commercial casein-carbon agar used in practical bacteriology for the diagnosis of pertussis has shown essential differences in the efficacies of various lots of this medium. Only 11 of the 30 tested lots provided the formation of B. pertussis colonies in the periods established by the instruction. Addition of antibiotics to the medium reduced its diagnostic characteristics still more.
