1.
Adv Exp Med Biol
; 486: 327-31, 2000.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11783509
Assuntos
Metiltransferases/genética , Metiltransferases/metabolismo , Leucemia-Linfoma Linfoblástico de Células Precursoras/enzimologia , Alelos , Antimetabólitos Antineoplásicos/uso terapêutico , Criança , Feminino , Testes Genéticos , Genótipo , Humanos , Masculino , Mercaptopurina/uso terapêutico , Metotrexato/uso terapêutico , Mutação , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética
2.
J Pharm Biomed Anal
; 15(6): 773-81, 1997 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-9172103
RESUMO
A high-performance liquid chromatographic method has been developed for the determination of 4-hydroxyifosfamide, a metabolite of ifosfamide, in plasma of cancer patients. The analyte is derivatized to 7-hydroxyquinoline, which can be detached fluorimetrically. The calibration graph is linear in the concentration range 0.05-25 microM, the limit of detection being 40 nM. Any inference from acrolein, another metabolite of ifosfamide, was ruled out. 4-Hydroxyifosfamide is very unstable in plasma and a stabilization procedure by adding citric acid has been developed. Thus treated, the samples were stable for 4 days. Analysis of a patient's plasma samples revealed that the 4-hydroxifosfamide concentration did not exceed 10 microM.
