RESUMO
Superparamagnetic nanoparticles varying by their chemical composition and synthesis method were used to transfer DNA into somatic cells under the influence of constant magnetic field (method of magnetofection). Magnetite particles obtained by mechanochemical synthesis ensured higher expression of the marker gene GFP (evaluated by fluorescence intensity of the cell lysate) then particles of ferric oxide obtained by chemical co-precipitation and cobalt ferrospinel particles obtained by the mechanochemical method.
Assuntos
DNA/metabolismo , Técnicas de Transferência de Genes , Proteínas de Fluorescência Verde/genética , Nanopartículas de Magnetita , Linhagem Celular , Cobalto , Portadores de Fármacos , Compostos Férricos/química , Óxido Ferroso-Férrico/química , Células HEK293 , Humanos , Fenômenos MagnéticosRESUMO
Contractile responses of airways segments of porpoises inhaling nanopowder CoFe2O4 were stidued by means of a mechanographic method. Inhalation of the nanosize particles of CoFe2O4 in vivo and in vitro testing the nanomaterial on isolated smooth muscles led to potentiation histaminergic, cholinergic contractile activity in airways of porpoises and to strengthening of adrenergic relaxing answers. Nanosize particles vary amplitude of hyperpotassium reductions in smooth muscle segments of airways similarly to the effect of depolymerizing drug colchicine.
Assuntos
Brônquios , Cobalto/efeitos adversos , Compostos Férricos/efeitos adversos , Contração Muscular/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Nanopartículas/efeitos adversos , Animais , Brônquios/efeitos dos fármacos , Cobalto/farmacologia , Compostos Férricos/farmacologia , Cobaias , Masculino , Contração Muscular/fisiologia , Músculo Liso/fisiologia , Nanopartículas/administração & dosagem , Traqueia/efeitos dos fármacos , Traqueia/fisiologiaRESUMO
The effect of nanopowder CoFe(2)O(4)on contractile responses of smooth-muscle segments of guinea pigs airways was studied by mechanography. Both in vivo inhalation of nanopowder aerosol or in vitro application of nanopowder to isolated airway segments increased the amplitude of contractile responses to histamine and potentiated the dilatory reaction to adrenergic salbutamol.
Assuntos
Nanopartículas Metálicas/toxicidade , Contração Muscular/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Fenômenos Fisiológicos Respiratórios/efeitos dos fármacos , Administração por Inalação , Albuterol/farmacologia , Animais , Cobalto , Cobaias , Histamina/farmacologia , Nanopartículas Metálicas/administração & dosagem , Contração Muscular/fisiologia , Músculo Liso/fisiologiaRESUMO
We studied in vitro effect of ferrimagnetic nanoparticles in a dose of 3 mg/liter (10 maximum permissible concentrations) on colony-forming capacity of bone marrow granulocytic and monocytic precursors in a constant magnetic field at magnetic field intensity of 200 Oe. We tested powders obtained by the methods of electrical explosion of conductors (magnetite and a mixture of hematite with magnetite) or mechanochemical synthesis (cobalt ferrite). According to electron microscopy, size of particles was within 6-65 nm. Specific effect of nanopowders on functional properties of hemopoietic and stromal cells were demonstrated; this effect was not related to dissolution of these powders, but had a complex nature. It depends on the size and magnetic characteristics of powder particles, the route and dose of administration, and the presence of external magnetic field. It was emphasized that in multicellular systems a reaction of committed hemopoietic precursors mediated via cells (factors) of microenvironment cannot be excluded, the state of this system varying in different individuals and under different conditions. Our data open new vistas for the creation and targeted use of nanosized materials and technologies for individual therapy in the context of personalized medicine.
