RESUMO
We studied the function of the antioxidant system in tumor cell lines MCF-7 and H1299 that differ by the state of tumor suppressor gene p53. Exposure to different classes of cytotoxic compounds induced several types of antioxidant system responses that depend on the type of cell line. The effects of platinum(II) and platinum(IV) complexes on activity of antioxidant enzymes vary, which can be explained by differences in their accumulation and biotransformation in tumor cells. Triazole and oxazolidinone derivatives had little effect on activity of superoxide dismutase and catalase in H1299 cells, but increased superoxide dismutase activity in MCF-7 cells.
Assuntos
Antineoplásicos/toxicidade , Catalase/metabolismo , Cisplatino/toxicidade , Compostos Organoplatínicos/toxicidade , Superóxido Dismutase/metabolismo , Indução Enzimática , Humanos , Concentração Inibidora 50 , Células MCF-7 , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismoRESUMO
The review describes the influence of serotonin (5-HT) and its receptor, transporter SERT and nuclear factor Nf-kB on the immune function of the body, particularly in the digestive tract. The mechanisms of the synthesis, metabolism and catab. olism of 5-HT are characterized. The targets for serotonin on cell level are immunoactive cells--neutrophils, eosinophils, leukocytes, monocytes and dendritic cells. Receptor and non-receptor described mechanisms of action of peripheral serotonin are described. immunoactive cells express on their membrane 5 HT1A-5-HT1E-5-HT2A-5-HT3-, 5-HT4- and 5-HT7-serotonin receptor subtypes. Activation of these receptors modulate the release of TNF-α, secretion of interleukins IL-1ß, IL-6, IL-8/CXCL8, IL-12p40. Mediators of the non-receptor mechanisms are transporter SERT, tryptophan hydroxylase-1 and nuclear factor Nf-kB. Target-genes for Nf-kB are genes encoding cytokines. The cooperation of non/receptory pathways involved 5-HT1A- and 5-HT3 receptors. It is concluded that the diversity of ways of peripheral serotonin on the immune function of the body are existed.
Assuntos
Sistema Imunitário/metabolismo , Serotonina/metabolismo , Mucosa Gástrica/imunologia , Mucosa Gástrica/metabolismo , Gastroenteropatias/imunologia , Gastroenteropatias/metabolismo , Humanos , Mucosa Intestinal/imunologia , Mucosa Intestinal/metabolismo , NF-kappa B/metabolismo , Receptores de Serotonina/metabolismo , Antagonistas da Serotonina/farmacologia , Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo , Agonistas do Receptor de Serotonina/farmacologiaRESUMO
Obesity and type 2 diabetes (DM2) in women are accompanied by atherogenic dyslipidemia, with the activation of lipid peroxidation (LPO), as well as disturbances in the antioxidant defense system (ADS). DM2 due to imbalance in LPO-ADS is accompanied by a high concentration of LPO products, decreased parameters of antioxidant systems and timpaired utilization of glucose by cells. The results obtained in this study suggest an important role of fatty acids and their metabolites in the pathogenesis of obesity and DM2, which should be taken into consideration during design and selection of appropriate preventive and therapeutic measures aimed at prevention or elimination of the violations.
