Potential application of cryogenic freezer on turbot sperm cryopreservation.

BACKGROUND: Cryopreservation is a common practice to preserve fish sperm for prolonged periods. OBJECTIVE: To examine the effect of different freezing protocols on sperm characteristics, fertilization, and hatching rate of turbot. MATERIALS AND METHODS: Milt was obtained from ten 8-year-old turbot (54.3 ± 1.7 cm in length and 3,106 ±283 g in weight) at the peak of spawning season. Six batches of milts with >90% motility was pooled and diluted to 1:3 by adding dimethyl sulfoxide (DMSO, 10%) as cryoprotectant. Then straws filled with semen were subjected to three freezing protocols (cooling rates). Sperm characteristics were assessed using sperm class analyzer before and after cryopreservation. Cryopreserved and fresh sperm were used for artificial fertilization to assess fertilization and hatching rates. RESULTS: Cryopreservation protocol has significantly deleterious effects on total motility, progressive motility, curvilinear velocity, straight line velocity, average path velocity, linearity index, straightness index, oscillation index, and amplitude of lateral head displacement of sperm. However, the beat frequency of cryopreserved sperm was found to be similar to control sperm. The fertilization rate of sperm subjected to three freezing protocols were similar, varying between 65.3 % and 75.6 %, and the hatching rates varied from 51.2 % to 70.7 %. CONCLUSION: The results show the potential application of cryopreservation in fish hatcheries. doi.org/10.54680/fr22410110312.

Assessment of the effects of radiofrequency radiation on human colon epithelium cells.

OBJECTIVES: The aim of the study was to investigate the possible effects of radiofrequency radiation (RFR) at different frequencies for different exposure durations on caspase-dependent apoptosis pathways in human colon adenocarcinoma (HT-29). METHODS: HT-29 cells were exposed to 1800 MHz; 2100 MHz and 2600 MHz RFR for 3 h cont., 6 h int. and 6 h cont.. Cell viability measurements were performed by Trypan Blue exclusion assay and the gene expressions of CASP8, CASP9, CASP3 and CASP12 were analyzed using qRT-PCR. RESULTS: Exposure to 2100 MHz RFR for all 3 durations of exposures was more effective for the ratio of the number of viable HT-29 cells w.r.t 1800 MHz RFR and 2600 MHz RFR exposures. After 2100 MHz RFR exposure, caspase activation increased significantly (for 3h cont. and 6 h int. exposures CASP8 and CASP9 levels; for 6 h cont. exposure CASP3 levels) (p 0.05). CONCLUSION: Decreases in the cell viability of HT-29 cells for certain frequencies and also durations are consistent with significant increases in caspase activations. The results of caspase activation after 1800 MHz or 2600 MHz RFR exposures can be interpreted as the activation of different types of cell death pathway by caspase signaling cascades (Fig. 15, Ref. 56).

Ulnar variance according to gender and side during aging: An analysis of 600 wrists.

BACKGROUND: Ulnar variance, or the difference in height between the joint surfaces of the distal radius and ulna, may play a role in several diseases of the wrist. HYPOTHESIS: This study was to conduct a detailed analysis of ulnar variance in a wider case series by dividing the patients' radiographs into groups according to age, gender, and side. MATERIALS AND METHODS: Twenty groups were created in order to investigate the change of ulnar variance according to age, gender, and side. Each group was planned to have 30 wrists. Wrist radiographs were scanned retrospectively, and ulnar variance was measured using the method of perpendiculars. Patients with any arthritic conditions, avascular necrosis, congenital deformities, bone and soft tissue tumors, and previous fractures or surgeries, and those radiographs, which were not at the exact anteroposterior position, and with insufficient technique were not included the study. RESULTS: Six hundrends wrists (300 males [150 right, and 150 left wrists], and 300 females [150 right, and 150 left wrists]) were included in this study. There was a significant difference between the males (median: 0.4, minimum: -3.8, maximum: 5.1) and females (median: 0.85, minimum: -4.8, maximum: 5.7), regardless of the age and side (p=0.043). In the right wrists only (regardless of age), there was also a significant difference between the males (median: 0, minimum: -3.8, maximum: 5.1) and females (median: 0.8, minimum: -3.9, maximum: 5.7) (p=0.025). No statistically significant differences were found within the males and females with regard to aging, or between the sides in the same sex in any decade. In addition, there were no significant differences between the same sides in the males and females in any decade. DISCUSSION: Although this study has one of the larger series in the literature, further studies should be done in different populations and ethnicities to obtain more accurate results, and to understand the relationship between ulnar variance and specific wrist disorders. LEVEL OF EVIDENCE: Level III.

Chronic subdural hematoma in adults: why does it occur more often in males than females? Influence of patient's sexual gender on occurrence.

AIM: Chronic subdural hematoma (CSDH) is a very common condition seen usually in the later stages of life. The goal of this study was to determine whether there is any influence of patient's sexual gender on occurrence of that hematoma. METHODS: The study was carried out on 76 consecutive adult patients (40-93-year-old, main 70.86) who underwent surgery for CSDH. The relationship between sex and occurrence was studied. The CSDH series appeared in the literature was also reviewed. Throughout the analysis, P<0.05 was considered statistically significant. RESULTS: A total of 62 (81.6%) patients were males and 14 (19.1%) females, with the male predominance occurring in all groups. The male to female ratio was 4:21. CONCLUSION: Male preponderance in CSDH provides valuable information about human cerebrum. Identification of this relationship may be useful to elucidate the origin and the pathogenesis of CSDH.

Reproductive decisions after prenatal diagnosis in neurofibromatosis type 1: importance of genetic counseling.

Neurofibromatosis type 1 (NF1) is one of the most common autosomal dominant disorders affecting approximately 1/3500 individuals in all ethnic groups. It is characterized by cutaneous and plexiform neurofibromas, café-au-lait spots, Lisch nodules, freckling in axillary and inguinal regions, optic gliomas and an increased risk of malignancy. The mutation rate of NF1 is one of the highest known for human disorders: approximately 50% of all affected individuals carry de novo mutations. Detection of disease causing mutations in the NF1 gene allows presymptomatic and prenatal diagnosis, but is complex and time-consuming due to the large size of the gene, the existence of pseudogenes, the lack of clustering of the mutations in a particular region of the gene, and the variability of clinical findings. Because the time for investigations in prenatal diagnosis is restricted, detection of disease-associated NF1 alleles is more rapid and useful especially for familial cases. Therefore, genetic diagnosis of NF1 is frequently performed by linkage analysis. In our laboratory, 37 families were characterized with this method, of which two requested prenatal diagnosis. One fetus was found to be under NF1 risk. However, parents elected to continue pregnancy: the child is now 2.5 years old and has NF1 features. The phenotypic variability and the absence of genotype-phenotype correlation create difficulties in reproductive decisions for NF1 families, underlining the importance of appropriate counseling and detailed discussion of possible outcomes before genetic testing of the fetus.

The prognostic importance of trauma scoring systems for blunt thoracic trauma.

BACKGROUND: Early identification and aggressive management of blunt thoracic trauma are essential to reduce the significant rates of morbidity and mortality. The aim of this study was to evaluate the independent predictive value of 5 different trauma scoring systems (Revised Trauma Score [RTS], Trauma and Injury Severity Score [TRISS], Injury Severity Score [ISS], Lung Injury Scale [LIS], and Chest Wall Injury Scale [CWIS]) with respect to prognostic factors such as tube thoracostomy duration, the need for mechanical support and thoracotomy, the length of hospital and ICU stay, morbid conditions, and deaths of patients with blunt thoracic trauma. METHODS: The records of 152 patients with blunt thoracic trauma were reviewed and data consisting of the patients' age and gender, blood pressure and respiratory rate on admission, the extent of chest wall and intrathoracic injury, types of associated injuries, Glasgow Coma Scale (GCS) scores, the need for mechanical support and thoracotomy, tube thoracostomy duration, length of hospital and ICU stay, morbid conditions, and deaths were collected. The relations between the trauma scoring systems and prognostic factors were evaluated by multivariate analysis. RESULTS: The analysis showed that only TRISS was an independent predictor of mortality and only LIS was an independent predictor of morbidity, the need for thoracotomy, and tube thoracostomy duration. TRISS and LIS were independent predictors of the length of ICU stay. ISS, CWIS, and LIS were independent predictors of the need for mechanical support. RTS, TRISS, ISS and LIS were independent predictors of the length of hospital stay. CONCLUSIONS: The LIS grade appeared to correlate with the severity of blunt thoracic injury and was found to be the most useful scoring system in predicting the outcomes of these patients.