RESUMO
We have examined the activation of glial cells and the upregulation of phosphorylated extracellular signaling-regulated kinase (ERK)-1 and -2 in upper thoracic segments of the spinal cord in rats following acute cardiac injury (ACI). ACI was established by intramyocardial injection of formalin and confirmed by hematoxylin and eosin (H&E) and terminal transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) staining. Following ACI, the astrocytes (determined by glial fibrillary acidic protein (GFAP) immunoreactivity (-IR)) and microglia (determined by OX-42-IR) were activated within the thoracic spinal cord. Phosphorylated (phospho-) ERK-IR was also activated in response to ACI. The upregulation of phospho-ERK was observed at 1h and became very obvious at 6h following ACI. The upregulated phospho-ERK was evidently expressed in the superficial and deep dorsal horn of the thoracic spinal cord. The activated ERK was also expressed in the intermediolateral nucleus (IML), nucleus intercalatus (IC) and the long processes projecting to the central canal, regions closely associated with autonomic outflow. Thus, the present study suggested that ACI could induce the activation of spinal ERK, which might link the nociceptive processing with the spinal sympathetic reflexes in myocardial injury in clinics.
Assuntos
Cardiopatias/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Neuroglia/fisiologia , Medula Espinal/metabolismo , Medula Espinal/patologia , Doença Aguda , Animais , Ativação Enzimática , Líquido Extracelular/metabolismo , Formaldeído , Cardiopatias/induzido quimicamente , Imuno-Histoquímica , Masculino , Proteína Quinase 3 Ativada por Mitógeno , Dor/induzido quimicamente , Dor/metabolismo , Fosforilação , Ratos , Ratos Wistar , Tórax , Regulação para CimaRESUMO
Domoic acid (DA), a potent neurotoxin, administered intravenously (0.75 mg/kg body weight) in adult rats evoked seizures accompanied by nerve cell damage in the present study. Neuronal degeneration and microglial reaction in the hippocampus were investigated, and the temporal profile of bcl-2, bax, and caspase-3 genes in cell death or survival was assessed following the administration of DA. Nissl staining showed darkly stained degenerating neurons in the hippocampus following the administration of DA at 1-21 days, the degeneration being most severe at 5 days. Ultrastructural study in CA1 and CA3 regions of hippocampus revealed two types of neuronal degeneration, cells that exhibited swollen morphology and shrunken electron-dense cells. Immunoreactivity of Bcl-2 and Bax was increased considerably at 16 hr and 24 hr in the neurons of the hippocampus following DA administration. No significant change was observed in the immunoreactivity of caspase-3 in the controls and DA-treated rats at any time interval. Microglial cells in the hippocampus showed intense immunoreaction with the antibodies OX-42 and OX-6 at 1-21 days after DA administration, indicating the up-regulation of complement type 3 receptors and major histocompatibility complex type II antigens for increased phagocytic activity and antigen presentation, respectively. Terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL) showed occasional positive neurons in the CA1 and CA3 regions at 5 days after DA administration, with no positive cells in the controls. RT-PCR analysis revealed that bcl-2 and bax mRNA transcripts in the hippocampus were significantly increased at 16 hr and gradually decreased at 24 hr following the administration of DA. Although bax and bcl-2 mRNA expression is rapidly induced at early stages, in situ hybridization analysis revealed complete loss of bcl-2, bax, and caspase-3 mRNA at 24 hr after DA administration in the region of neuronal degeneration in the hippocampus. These results indicate that the pattern of neuronal degeneration observed during DA-induced excitotoxic damage is mostly necrotic.