RESUMO
Eight puppies (group 1) were vaccinated once with a bivalent modified-live vaccine against infectious tracheobronchitis by the intranasal route and at the same time with an injectable trivalent vaccine against canine parvovirus, canine distemper virus and canine adenovirus; a second group of eight puppies (group 2) was vaccinated only with the intranasal bivalent vaccine, and a further eight puppies (group 3) were vaccinated only with the injectable trivalent vaccine. Three weeks later they were all challenged with wildtype Bordetella bronchiseptica and canine parainfluenza virus by the aerosol route, and their antibody responses to the five vaccine organisms were determined. Oronasal swabs were taken regularly before and after the challenge for the isolation of bacteria and viruses, and the puppies were observed for clinical signs for three weeks after the challenge. There were no significant differences in the puppies' titres against canine parvovirus, canine distemper virus and canine adenovirus type 2 between the groups vaccinated with or without the bivalent intranasal vaccine. After the challenge the mean clinical scores of the two groups vaccinated with the intranasal vaccine were nearly 90 per cent lower (P=0.001) than the mean score of the group vaccinated with only the trivalent injectable vaccine, and the puppies in this group all became culture-positive for B bronchiseptica and canine parainfluenza virus. There were only small differences between the rates of isolation of B bronchiseptica from groups 1, 2 and 3, but significantly lower yields of canine parainfluenza virus were isolated from groups 1 and 2 than from group 3.
Assuntos
Vacinas Bacterianas/administração & dosagem , Infecções por Bordetella/veterinária , Bordetella bronchiseptica/imunologia , Doenças do Cão/prevenção & controle , Vacinas Virais/administração & dosagem , Infecções por Adenoviridae/prevenção & controle , Infecções por Adenoviridae/veterinária , Adenovirus Caninos/imunologia , Administração Intranasal , Animais , Animais Recém-Nascidos , Anticorpos Antibacterianos/sangue , Anticorpos Antivirais/sangue , Infecções por Bordetella/prevenção & controle , Cinomose/prevenção & controle , Vírus da Cinomose Canina/imunologia , Cães , Feminino , Herpesvirus Canídeo 1/imunologia , Masculino , Vacinas contra Parainfluenza , Infecções por Paramyxoviridae/prevenção & controle , Infecções por Paramyxoviridae/veterinária , Infecções por Parvoviridae/prevenção & controle , Infecções por Parvoviridae/veterinária , Parvovirus Canino/imunologia , Organismos Livres de Patógenos Específicos , Vacinas Atenuadas/administração & dosagemRESUMO
Twelve specific pathogen-free (spf) puppies were vaccinated intranasally with a bivalent, modified live vaccine against infectious tracheobronchitis (group 1) and six puppies of the same age and from the same source served as unvaccinated controls (group 2). Both groups were challenged with wild-type Bordetella bronchiseptica and canine parainfluenza virus by the aerosol route 56 weeks after group 1 had been vaccinated, and at the same time six 10-week-old spf puppies from the same source (group 3) were also challenged. Oronasal swabs were taken regularly before and after the challenge, for the isolation of bacteria and viruses, and the dogs were observed for clinical signs for three weeks after the challenge. The control dogs became culture-positive for B bronchiseptica and canine parainfluenza virus, but the isolation yields from the vaccinated group were significantly lower (P<0.05). The mean clinical scores of the vaccinated group were 61 per cent lower than the scores of group 2 (P=0.009), and 90 per cent lower than the scores of group 3 (P=0.001).