Swept-wavelength null polarimetry for highly sensitive birefringence laser scanning microscopy.

We have recently demonstrated a high-speed null polarimeter [Opt. Express30, 18889 (2022)10.1364/OE.454193OPEXFF1094-4087] based on passive polarization optics and using a fast swept-wavelength laser source. We report here its implementation in a laser-scanning microscope setup, enabling highly sensitive linear retardance imaging with a pixel dwell time of 10 µs. The instrument is also able to measure light depolarization induced by the sample. Images of biological samples, including cancerous tissue and cells, illustrate its performances.

Systematic errors in a swept-wavelength null polarimeter for weak linear retardance measurements.

We recently developed a high speed null polarimeter [Opt. Express30, 18889 (2022)OPEXFF1094-408710.1364/OE.454193] based on passive polarization optics and a high speed wavelength swept laser source, enabling the measurement of linear retardance with 3.1µd e g/H z resolution within a minimum acquisition time of 10 µs, corresponding to a linear retardation of 8.6×10-9 λ/H z. The counterpart of high sensitivity lies in the systematic errors unlike Mueller polarimeters, which can be calibrated but which are much less sensitive. This paper focuses on the accuracy of this null polarimeter and provides hardware and numerical solutions to improve both linear retardance and azimuth measurements. Experiments and theoretical simulations are carried out to demonstrate the relevancy of these solutions.

Swept-wavelength null polarimeter for high-speed weak anisotropy measurements.

Null-polarimeters provide the best sensitivity to anisotropy measurements and so far have been developed for the detection of small optical activities. This paper revisits null polarimetry through an original configuration based on the concept of spectrally encoded light polarization, in order to measure, with unprecedented speed, either linear or circular retardance with the same degree of sensitivity . Using passive polarization optics and a high speed wavelength swept laser source, the achieved single-pass sensitivity was 55nrad/Hz and 45nrad/Hz for respectively linear and circular retardance considering a minimum acquisition time of 10 µs. Due to its compactness and rapidity, the method could be further implemented in laser scanning microscopes, which should be of great interest for revealing very low anisotropies in biological tissues.