Complement regulatory protein CD46 promotes bladder cancer metastasis through activation of MMP9.

CD46, a transmembrane protein known for protecting cells from complement­mediated damage, is frequently dysregulated in various types of cancer. Its overexpression in bladder cancers safeguards the cancer cells against both complement and antibody­mediated cytotoxicity. The present study explored a new role of CD46 in facilitating cancer cell invasion and metastasis, examining its regulatory effect on matrix metalloproteases (MMPs) and their effect on the metastatic capability of bladder cancer cells. Specifically, CD46 alteration positively influenced MMP9 expression, but not MMP2, in several bladder cancer cell lines. Furthermore, CD46 overexpression triggered phosphorylation of p38 MAPK and protein kinase B (AKT), leading to enhanced activator protein 1 (AP­1) activity via c­Jun upregulation. The inhibition of p38 or AKT pathways attenuated the CD46­induced MMP9 and AP­1 upregulation, indicating that the promotion of MMP9 by CD46 involved activating both p38 MAPK and AKT. Functionally, the upregulation of MMP9 by CD46 translated to increased migratory and invasive capabilities of bladder cancer cells, as well as enhanced in vivo metastasis. Overall, the present study revealed a novel role for CD46 as a metastasis promoter through MMP9 activation in bladder cancers and highlighted the regulatory mechanism of CD46­mediated MMP9 promotion via p38 MAPK and AKT activation.

Emotional - Behavioral Difficulties and Prosocial Behaviour among Vietnamese Adolescents: the Role of Social Support.

Background: In recent decades, a high prevalence of mental health problems among adolescents has been reported worldwide. In Vietnam, mental health problems such as emotional and behavioural difficulties are relatively common in the adolescent age group. Objective: This study aimed to estimate the prevalence of emotional-behavioural problems and prosocial behaviours in Vietnamese adolescents and identify the role of social support in these adolescents' strengths and difficulties. Methods: A population sample of 582 adolescents from sixth to ninth grade from four secondary schools in Vietnam voluntarily participated by completing the Strengths and Difficulties Questionnaire (α = .64) and the Social Support Scale (α = .90). Results: The research results revealed that Vietnamese adolescents suffered from considerable levels of emotional-behavioural difficulties and prosocial behaviour problems. Through multible regression analysis, support from their families significantly predicted to decrease students' emotional and behavioural symptoms (ß\< 0; p\< .05) and increasing prosocial behaviours (ß> 0; p \< .05). In contrast, friend support only contribute to protecting adolescents from behavioural problems (ß = -.18; p \< .05) and encouraged prosocial behaviours (ß = .22; p \< .05). Discussion: Family support had a central role in its positive contribution to reducing emotional-behavioural and prosocial behaviour problems. Friend support only decreased behavioural problems and encouraged prosocial behaviours. These results are interpreted that educators could limit adolescents' emotional-behavioural difficulties and encourage prosocial behaviours through social support from their families and friends.

Effects of digital parenting interventions on self-efficacy, social support, and depressive symptoms in the transition to parenthood: A systematic review and meta-analysis.

BACKGROUND: Parenting self-efficacy is essential for the transition to parenthood. As digital parenting educational interventions are rapidly being developed, their effects have not been examined by pooling available randomized controlled trials (RCTs). OBJECTIVES: To comprehensively investigate the effects of digital educational interventions on parents' self-efficacy, social support, and depressive symptoms in the first year after childbirth and identify the significant associated factors. METHODS: This study searched six electronic databases for relevant RCTs examining the efficacy of digital parenting interventions from inception to September 2022. The studied outcomes included changes in parent's self-efficacy, social support, and depressive symptoms observed after participating in a digital parenting program. The random-effects model was used to pool results. Subgroup and moderator analyses were performed. RESULTS: In total, seven RCTs enrolling 1342 participants were included. The parents who received digital parenting interventions had higher parenting self-efficacy (standardized mean difference [SMD]: 1.06, 95 % confidence interval [CI]: 0.40-1.71, p =.002) and social support (SMD: 2.72, 95 % CI: 0.38-5.07, p =.02) and decreased depressive symptoms at 3 months postpartum (SMD: -0.39, 95 % CI: -0.73 to - 0.04, p =.03). Providing the interventions for ≥ 6 weeks (SMD: 1.62, 95 % CI: 1.18-2.06, p <.001), providing in-person orientation (SMD: 1.88, 95 % CI: 1.32-2.44, p <.001), including a guided curriculum (SMD: 2.00, 95 % CI: 1.78-2.22, p <.001), and conducting interventions in Organisation for Economic Co-operation and Development countries (SMD: 1.98, 95 % CI: 1.78-2.19, p <.001) were identified as significant moderators. CONCLUSIONS: Digital parenting interventions significantly increase parenting self-efficacy and social support as well as alleviate depressive symptoms for parents during their first year after childbirth. Such interventions can be beneficial for parents who prefer online education. Future studies investigating the long-term effects of these interventions are warranted. REGISTRATION: The protocol for this systematic review and meta-analysis is registered in PROSPERO (registration number: CRD42021243641).

A large-scale MIMO antenna system for 5G IoT applications.

A multiple-input-multiple-output (MIMO) antenna system with low-profile and small element spacing characteristics is presented in this paper. This antenna contains multiple elements arranged in both E-plane and H-plane configurations. The original strong coupling between the MIMO elements can be suppressed by exciting orthogonal operating modes. To achieve this, a half-wavelength microstrip line and a quarter-wavelength grounded stub are utilized to decouple the H- and E-plane MIMO arrays. A 2 × 2 antenna prototype is fabricated and measured to demonstrate the decoupling concept's feasibility. The measured impedance bandwidth is from 4.78 to 4.81 GHz. Across this band, the isolation is better than 15 dB with extremely small edge-to-edge distances of 0.032λ and 0.026λ in the E- and H-plane, respectively. Featuring the simple decoupling structure, small element spacing, and the capability of extending to a large-scale 2 × N array, the proposed antenna can be used for 5G Internet of Things (IoT) applications operating at the N79 frequency band.

Garcicowanones C-E, three new hydrated-geranylated xanthones from the roots of Garcinia cowa Roxb. ex Choisy, and their α-glucosidase inhibition activities.

Three new xanthones, garcicowanones C-E (1 - 3), and six known xanthones (4 - 9) were isolated from the roots of Garcinia cowa Roxb. ex Choisy. Their chemical structures were determined using spectroscopic technics, including HR-ESI-MS and 2 D NMR. All isolated compounds were evaluated for in vitro α-glucosidase inhibition. Cowanol (6) and norcowanin (8) had the most potent α-glucosidase inhibitory activity, with respective IC50 values of 33.5 ± 0.8 and 17.2 ± 0.3 µM, compared with the positive control, acarbose (IC50 257.3 ± 4.8 µM).

S/Mo ratio and petal size controlled MoS2 nanoflowers with low temperature metal organic chemical vapor deposition and their application in solar cells.

Vertically aligned two-dimensional (2D) molybdenum disulfide nanoflowers (MoS2 NFs) have drawn considerable attention as a novel functional material with potential for next-generation applications owing to their inherently distinctive structure and extraordinary properties. We report a simple metal organic chemical vapor deposition (MOCVD) method that can grow high crystal quality, large-scale and highly homogeneous MoS2 NFs through precisely controlling the partial pressure ratio of H2S reaction gas, P SR, to Mo(CO)6 precursor, P MoP, at a substrate temperature of 250 °C. We investigate microscopically and spectroscopically that the S/Mo ratio, optical properties and orientation of the grown MoS2 NFs can be controlled by adjusting the partial pressure ratio, P SR/P MoP. It is also shown that the low temperature MOCVD (LT-MOCVD) growth method can regulate the petal size of MoS2 NFs through the growth time, thereby controlling photoluminescence intensity. More importantly, the MoS2 NFs/GaAs heterojunction flexible solar cell exhibiting a power conversion efficiency of ∼1.3% under air mass 1.5 G illumination demonstrates the utility of the LT-MOCVD method that enables the direct growth of MoS2 NFs on the flexible devices. Our work can pave the way for practical, easy-to-fabricate 2D materials integrated flexible devices in optical and photonic applications.

Seroprevalence of Toxoplasma gondii infection from water buffaloes (Bubalus bubalis) in northeastern and southern Thailand.

Toxoplasmosis is a parasitic disease resulting from infection with the apicomplexan Toxoplasma gondii (Nicolle et Manceaux, 1908), one of the world's most common parasites in warm-blooded animals, including humans. Sources of infection can be exposed to infected cat faeces, mother-to-child transmission during pregnancy, and notably, the consumption of undercooked contaminated meat of intermediate hosts. In Thailand, water buffaloes are highly valued for rice cultivation, traditional culture and meat production. Like several other mammalian species, these animals play a role as reservoirs of T. gondii, thus representing a threat to human health. The seroprevalence of T. gondii infection in swamp buffaloes was examined in southern and northeastern Thailand. In total, serum samples of 721 water buffaloes were collected from seven provinces (Ubon Ratchathani, Roi Et, Si Sa Ket, Surin, Buri Ram, Sakon Nakhon, and Songkhla) and examined for the presence of T. gondii infection using commercial latex agglutination test kits (TOXOCHECK-MT, Eiken Chemical Co., Tokyo, Japan). Of the 721 animals analysed, 49 (6.8%) were positive for T. gondii. Songkhla province had the highest seroprevalence (14.7%) among the seven provinces covered in this survey. There was a potential risk to local citizens of T. gondii infection identified by the present study, notably in northeastern Thailand, where despite lower seroprevalence consuming raw buffalo meat salad should be restricted to avoid the risk of zoonotic infections.

Expression of N-Acetylgalactosaminyltransferase-6 Is Related to Expression of Cell Adhesion Molecules in Endometrial Cancer.

BACKGROUND: The aberrant glycosylation of mucin type O-glycans is thought to be associated with functional alteration of cancer cells, including adhesive properties, as well as their potential for invasion and metastasis. Positive expression of N-acetylgalactosaminyltransferase-6 (GalNAc-T6) may also be a marker for aberrant O-glycans in carcinogenesis. We previously reported that over-expression of GalNAc-T6 had a strong association with endometrial cell invasion ability in vitro. MATERIALS AND METHODS: This study investigated the relationship between GalNAc-T6 expression and cell adhesion molecules in 218 endometrial carcinomas by immunohistochemistry. RESULTS: Expression of GalNAc-T6 was found to be significantly related to expression of E-cadherin. Positive expression of GalNAc-T6 was significantly associated with better histological grade and good clinical prognosis of patients, but positive E-cadherin and ß-catenin expression were not significantly associated with improved overall survival. CONCLUSION: GalNAc-T6 might be related to cell-cell adhesion in the early phase of cancer invasion in endometrial carcinoma.

Bacterial load of pneumococcal serotypes correlates with their prevalence and multiple serotypes is associated with acute respiratory infections among children less than 5 years of age.

BACKGROUND: Among pneumococcal serotypes, some serotypes are more prevalent in the nasopharynx than others; determining factors for higher prevalence remain to be fully explored. As non-vaccine serotypes have emerged after the introduction of 7-valent conjugate vaccines, study of serotype specific epidemiology is in need. When two or more serotypes co-colonize, they evolve rapidly to defend host's immune responses; however, a clear association of co-colonization with a clinical outcome is lacking. METHODS: Children less than 5 years old who were admitted to hospital due to acute respiratory infections (ARI) (n = 595) and healthy children (n = 350) were recruited. Carriage of pneumococcus was determined by culture and lytA PCR in the nasopharyngeal samples. Serotype/serogroup detection and its quantification were done by the nanofluidic real time PCR system. Spearman's correlation and logistic regression were used to examine a correlation of serotype/serogroup specific bacterial load with its prevalence and an association of co-colonization with ARI respectively. RESULTS: Serotype/serogroup specific bacterial load was correlated with its prevalence, both in ARI cases (Spearman's rho = 0.44, n = 186; P<0.0001) and healthy children (Spearman's rho = 0.41, n = 115; P<0.0001). The prevalence of multiple serotypes was more common in ARI cases than in healthy children (18.5% vs 7.1%; aOR 2.92, 95% CI: 1.27-6.71; P = 0.01). The dominant serotype in the co-colonization had a 2 log10 higher bacterial load than the subdominant serotype, both in ARI cases (P<0.001) and healthy children (P<0.05). CONCLUSIONS: High bacterial load in the nasopharynx may help transmit pneumococci among hosts, and increase the chance of successful acquisition and colonization. Co-colonization of multiple serotypes of pneumococci is linked with ARI, which infers the interactions of multiple serotypes may increase their pathogenicity; however, they may compete for growth in number.

A New Species of Graphis and New Lichen Records from Vietnam, Including a Second Worldwide Report of Sarcographina cyclospora

Graphis upretii is a new lichen species discovered in Vietnam. The species is characterized by a loosely corticate, rough, whitish grey to greyish green thallus, elongate and irregularly branched lirellae with an apically thin complete thalline margin (negrosina morph), laterally carbonized, entire proper exciple, clear hymenium, hyaline, 16~20 transversely locular ascospores, and about 50~95 x 10~15 microm in size. In addition, members of the taxon produce norstictic and stictic acids. Currently, the lichen flora of Vietnam include Arthonia radiata, Brigantiaea tricolor, Coenogonium implexum, Dirina paradoxa, Herpothallon sipmanii, Pertusaria pertusa, and Sarcographina cyclospora.

Two New Records of the Lichen Genus Placynthiella Elenkin in South Korea

This study describes two new records of the lichen genus Placynthiella (P. hyporhoda and P. icmalea) from South Korea. A brief taxonomic description and comments are provided for the new records. An artificial key is also provided for known species of this genus in South Korea.

Identification of Sculptolumina japonica (Physciaceae) in South Korea

The paper describes identification of new microlichen (Sculptolumina japonica) in South Korea. A detailed taxonomic description and comments are presented for the taxa studied. Lichen genus Sculptolumina is reported for the first time for this country.

Notes on the Existence of Leucodecton desquamescens (Thelotremoid Graphidaceae) in South Korea

This study describes a new record of a thelotremoid lichen (Leucodecton desquamescens) from South Korea. The taxon is characterized by thick, bulging thallus with many calcium oxalate crystal inclusions; an immersed, round to irregular ascomata with free exciple, ellipsoid to +/- roundish submuriform, brown ascospores, and lack of secondary metabolites. A detailed taxonomic description and comments are presented for this taxon. The lichen genus Leucodecton is reported for the first time in South Korea.