Improved Bowel Function in Patients with Spina Bifida After Bone Marrow-Derived Mononuclear Cell Transplantation: A Report of 2 Cases.

BACKGROUND Bowel dysfunction is observed in 42.2-71.2% of patients with spina bifida. Traditional treatments yield limited results. The objective of this paper is to report on improvement in bowel function in 2 children with spina bifida following bone marrow-derived mononuclear cells transplantation. CASE REPORT Two patients - 14 years old and 11 years old - with bowel dysfunction after myelomeningocele repair underwent 2 BMMNC transplantations without complications. Those patients had normal defecation, assessed through follow-ups of 21 months and 16 months, respectively. CONCLUSIONS BMMNC transplantation can improve bowel function, as demonstrated in 2 patients with spina bifida.

Monitoring of tritium concentration in Hanoi's precipitation from 2011 to 2016.

Tritium is a radioisotope of hydrogen and a component of the water molecule. It is a marker for reservoirs such as the stratosphere, troposphere, and oceans involved in the hydrological cycle. Tritium monitoring is an essential research tool in hydro-climate, dating for water and recharge groundwater. The Isotope Hydrology Laboratory has collected monthly precipitation samples in Hanoi for tritium concentration analysis. This paper reports the tritium concentrations in precipitation in the city from 2011 to 2016. The results show that monthly tritium concentration reached a maximum of 7.07 Tritium Units (TU) in August 2011. The mean annual tritium concentration stabilized from 2.03 to 3.36 TU. It suggests that tritium in monitoring station precipitation is predominantly natural. The seasonal variation trend of 3H in precipitation at the Hanoi station is similar to those monitored at the Hong Kong station. The correlation of tritium and rainfall was also estimated.

Tetanus toxin fragment C fused to flagellin makes a potent mucosal vaccine

PURPOSE: Recombinant subunit vaccines provide safe and targeted protection against microbial infections. However, the protective efficacy of recombinant subunit vaccines tends to be less potent than the whole cell vaccines, especially when they are administered through mucosal routes. We have reported that a bacterial flagellin has strong mucosal adjuvant activity to induce protective immune responses. In this study, we tested whether FlaB could be used as a fusion partner of subunit vaccine for tetanus. MATERIALS AND METHODS: We constructed fusion proteins consisted with tetanus toxin fragment C (TTFC), the nontoxic C-terminal portion of tetanus toxin, and a Toll-like receptor 5 agonist from Vibrio vulnificus (FlaB). Mice were intranasally administered with fusion protein and protective immune responses of the vaccinated mice were analyzed. RESULTS: FlaB-TTFC recombinant protein induced strong tetanus-specific antibody responses in both systemic and mucosal compartments and prolonged the survival of mice after challenge with a supra-lethal dose of tetanus toxin. CONCLUSION: This study establishes FlaB as a successful fusion partner for recombinant subunit tetanus vaccine applicable through mucosal route, and it further endorses our previous observations that FlaB could be a stable adjuvant partner for mucosal vaccines.