RESUMO
Pleural mesothelial cells (PMC) from the parietal pleura of rats were incubated in culture with UICC A chrysotile fibers. The sequence of events in phagocytosis was studied by electron microscopy: phases of attachment sequestration, and degranulation of lysosomal content into the phagocytic vacuole were observed. This demonstrates that PMC can engage in phagocytosis of chrysotile fibers.
Assuntos
Amianto , Fagocitose , Pleura/citologia , Animais , Células Cultivadas , Células Epiteliais , Microscopia Eletrônica , RatosRESUMO
Cultures were made of mesothelial cells obtained by scraping the parietal pleura of the adult rats. The growth was restricted to close polyhedric epithelial-like cells, forming a monolayer. The cellular proliferation continued until the 7th day, followed by a stationary phases. In subcultures the mesothelial cells kept their epithelial type. The cultures were stopped on the 20th day.