UV-B priming of Oryza sativa var. Kanchana seedlings augments its antioxidative potential and gene expression of stress-response proteins under various abiotic stresses.

Priming is one of the mechanisms for the induction of the antioxidant defense system and various stress-responsive proteins which help plants to survive under various abiotic stresses. Based on the observation that the rice seedlings primed with UV-B (low dose of UV-B irradiation-6 kJm-2) induced the acclimation against NaCl, PEG and UV-B stresses, it was of interest to see the augmentation of antioxidative potential and stress-responsive proteins accumulation in rice seedlings due to UV-B priming under these stresses. Various stresses result in production of ROS, which cause membrane degradation resulting in the accumulation of malondialdehyde. These negative impacts were observed exceedingly in rice seedlings from non-primed PEG stress (NP+P) condition than UV-B and NaCl stresses. The production of non-enzymatic antioxidants, activity/mRNA-level expressions of enzymatic antioxidants and stress-responsive proteins were effectively augmented in UV-B-primed rice seedlings subjected to NaCl stress (P+N) condition followed by UV-B stress (P+U) and PEG stress (P+P). The activation of stress-responsive proteins (HSP and LEA) in rice due to the UV-B priming of rice seedlings is being reported for the first time. The results revealed that the UV-B seedling priming was alleviating the effect of NaCl, PEG, and UV-B stresses in rice seedlings. The positive impacts of UV-B seedling priming were more prominent in rice seedlings subjected to NaCl stress, indicating the cross tolerance imparted by UV-B priming.

Amplification of abiotic stress tolerance potential in rice seedlings with a low dose of UV-B seed priming.

UV-B radiation is a major abiotic stress factor that adversely affects the growth and productivity of crop plants including rice (Oryza sativa L.). However, on the other hand, lower doses of UV-B radiation applied to seeds can have a priming effect on plants emerging from it. In this study, seeds of O. sativa var. kanchana were primed with UV-B radiation (6 kJ m-2) and were further subjected to NaCl, polyethylene glycol 6000 (PEG) and UV-B stress. The effects of UV-B priming in imparting NaCl, PEG and UV-B stress tolerance to rice seedlings were analysed through various photosynthetic features and antioxidative mechanisms. PSI and PSII activity levels as well as chl a fluorescence were found to be significantly higher in the UV-B primed and unstressed seedlings. When stress (NaCl, PEG and high UV-B) was imposed, increased PSI and PSII activity levels, chl a fluorescence and metabolite accumulation (proline, total phenolics and sugar) as well as nonenzymatic (ascorbate and glutathione) and enzymatic (superoxide dismutase, catalase, ascorbate peroxidase) antioxidants were recorded in UV-B primed and NaCl-stressed plants followed by UV-B primed and UV-B-stressed plants, and primed and PEG-stressed, compared with unprimed and stressed conditions. The results indicate that UV-B priming in rice seedlings effectively enhances the NaCl stress tolerance potential in rice to a greater extent than UV-B and PEG stress tolerance potential. The cost-effectiveness of UV-B seed priming is predominantly clear from the differing tolerance responses of rice seedlings exposed to different stress conditions.