Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Parasitology ; 149(2): 261-269, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34709144

RESUMO

In the light of global biodiversity change and emerging disease, there is an urgent need to establish efficient monitoring programmes of parasites in aquatic ecosystems. However, parasite identification is time-consuming, requires a high degree of taxonomic expertize and in general requires lethal sampling. The use of environmental DNA methodology to identify parasites has the potential to circumvent these limitations. This study evaluates the use of eDNA metabarcoding to detect the presence of all species of nematode and platyhelminth parasites in two New Zealand lakes. We developed two novel metabarcoding primer pairs targeting a region of cytochrome oxidase I gene (COI) specific to platyhelminths and nematodes. We successfully detected parasite DNA in both lakes. Platyhelminth DNA yield was in general greater than nematode DNA yield. This most likely results from the larger biomass of the former quantified using traditional methods, or the presence of free-swimming life stages in the life cycle of many platyhelminths. By using eDNA, we did not detect all expected parasite families revealed through traditional methods, likely due to a lack of sequencing data available from public databases such as GenBank. As such, genetic resources need to include full reference sequences if parasitology is to truly harness eDNA to characterize and monitor parasite biodiversity in natural systems.


Assuntos
DNA Ambiental , Parasitos , Animais , Biodiversidade , Código de Barras de DNA Taxonômico/métodos , Ecossistema , Monitoramento Ambiental/métodos , Humanos , Parasitos/genética , Água
2.
Parasitology ; 148(11): 1313-1319, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34103103

RESUMO

Every internet search query made out of curiosity by anyone who observed something in nature, as well as every photo uploaded to the internet, constitutes a data point of potential use to scientists. Researchers have now begun to exploit the vast online data accumulated through passive crowdsourcing for studies in ecology and epidemiology. Here, we demonstrate the usefulness of iParasitology, i.e. the use of internet data for tests of parasitological hypotheses, using hairworms (phylum Nematomorpha) as examples. These large worms are easily noticeable by people in general, and thus likely to generate interest on the internet. First, we show that internet search queries (collated with Google Trends) and photos uploaded to the internet (specifically, to the iNaturalist platform) point to parts of North America with many sightings of hairworms by the public, but few to no records in the scientific literature. Second, we demonstrate that internet searches predict seasonal peaks in hairworm abundance that accurately match scientific data. Finally, photos uploaded to the internet by non-scientists can provide reliable data on the host taxa that hairworms most frequently parasitize, and also identify hosts that appear to have been neglected by scientific studies. Our findings suggest that for any parasite group likely to be noticeable by non-scientists, information accumulating through internet search activity, photo uploads, social media or any other format available online, represents a valuable source of data that can complement traditional scientific data sources in parasitology.


Assuntos
Crowdsourcing/estatística & dados numéricos , Helmintos/fisiologia , Interações Hospedeiro-Parasita , Internet/estatística & dados numéricos , Animais , Canadá , Bases de Dados Factuais/estatística & dados numéricos , Demografia , Feminino , Humanos , Masculino , Fotografação , Densidade Demográfica , Estações do Ano , Fatores de Tempo , Estados Unidos
3.
Trends Parasitol ; 37(4): 267-272, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33547010

RESUMO

Digital data (internet queries, page views, social media posts, images) are accumulating online at increasing rates. Tools for compiling these data and extracting their metadata are now readily available. We highlight the possibilities and limitations of internet data to reveal patterns in host-parasite interactions and encourage parasitologists to embrace iParasitology.


Assuntos
Mineração de Dados , Parasitologia , Mineração de Dados/tendências , Interações Hospedeiro-Parasita , Internet , Parasitologia/métodos , Parasitologia/tendências
4.
Mitochondrial DNA A DNA Mapp Seq Anal ; 27(3): 1959-60, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-25329254

RESUMO

Three individual-specific DNA libraries of the deep-sea scleractinian coral Solenosmilia variabilis (Duncan, 1873) were constructed to obtain complete mitochondrial genomes using the 454 Life Science pyrosequencing system. Two mitogenomes were successfully assembled: both were 15,968 bp in length, with base composition of A (24.2%), T (41.1%), C (13.7%) and G (21.0%). The genome contains 13 protein-coding genes, 2 ribosomal RNA genes, 2 transfer RNA genes and a D-loop region. The two mitogenomes were almost identical, with only 5 nucleotide differences (0.03%), including a synonymous substitution within the nad1, nad2 and nad4L genes, and two transversions in the D-loop region. This inter-individual variation indicates that these genes and/or region are potential candidates as molecular markers for population genetic research. The mitogenome of S. variabilis will be useful for future phylogenetic and phylogeographic studies of deep-sea corals.


Assuntos
Antozoários/genética , Variação Genética , Genoma Mitocondrial , Animais , Pareamento de Bases/genética , Sequência de Bases , DNA Mitocondrial/genética , Genes Mitocondriais , RNA Ribossômico/genética , RNA de Transferência/genética
5.
Mitochondrial DNA A DNA Mapp Seq Anal ; 27(3): 1658-9, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-25228376

RESUMO

The complete mitochondrial genome of a New Zealand specimen of the deep-sea sponge Poecillastra laminaris (Sollas, 1886) (Astrophorida, Vulcanellidae), from the Colville Ridge, New Zealand, was sequenced using the 454 Life Science pyrosequencing system. To identify homologous mitochondrial sequences, the 454 reads were mapped to the complete mitochondrial genome sequence of Geodia neptuni (GeneBank No. NC_006990). The P. laminaris genome is 18,413 bp in length and includes 14 protein-coding genes, 24 transfer RNA genes and 2 ribosomal RNA genes. Gene order resembled that of other demosponges. The base composition of the genome is A (29.1%), T (35.2%), C (14.0%) and G (21.7%). This is the second published mitogenome for a sponge of the order Astrophorida and will be useful in future phylogenetic analysis of deep-sea sponges.


Assuntos
Genoma Mitocondrial , Poríferos/genética , Animais , Pareamento de Bases/genética , Sequência de Bases , DNA Mitocondrial/genética , Genes Mitocondriais
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...