Chitosan as biomaterial in drug delivery and tissue engineering.

Chitin is one of the most abundant polysaccharide found on earth. The deacetylated form of chitin viz. chitosan has been reported for its various important pharmacological properties and its role in tissue engineering and regenerative medicine is also well documented. Chitosan based bone graft substitutes are biocompatible, biodegradable, osteoconductive, osteoinductive and structurally similar to bone, with excellent mechanical strength and cost effectiveness. Chitosan based hydrogels and wound healing bandages have also found a great market in the field of medicine. More recently, chitosan has gained popularity for its use as a matrix molecule for drug delivery and also finds an upcoming utility in the area of dentistry. The present article has tried to review the latest research on chitosan based tissue engineering constructs, drug delivery vehicles as well as dental care products. An attempt has also been made to discuss the various modifications of chitosan that enhance its use for a given set of applications which would pave a way for future applied research in the field of biomedical innovation and regenerative medicine.

Cytomorphometric Analysis on the Effects of Components of Orthodontic Appliances on the Epithelial Cells of the Buccal Mucosa.

AIM: The aim of the present study was to evaluate the effect of fixed orthodontic appliances on the epithelial cells of buccal mucosa in patients undergoing orthodontic treatment. MATERIALS AND METHODS: The study group included 30 healthy patients who were advised orthodontic treatment. Applying sterile cement spatula, scrapping of exfoliated buccal epithelial cells was performed from the middle part of the inner cheeks before starting the orthodontic treatment and at 1st week, 2nd week, 1 month, and 45 days after the treatment, followed by smearing and staining with Papanicolaou stain. Light microscope was used to score micronuclei, and independent two-tailed t-test was used for statistical analysis. RESULTS: Micronuclei were assessed during the various stages of treatment and were recorded accordingly. At 1 week, there was increase in micronuclei number compared to before starting the treatment (day 0) and at 15th day; 30th day showed decrease in number compared to 1 week but increase compared to day 0. The results of day 45 were almost equal to day 0 with a significant P value (P < 0.001). CONCLUSION: Fixed orthodontic appliances induce increased micronuclei frequency, especially in the first weeks of treatment, however, these genotoxic effects tend to approach baseline levels in later period.

Piezosurgery: A Boon for Modern Periodontics.

Dentistry has undergone significant advancement and has seen several changing concepts over a decade. One such novel innovation is piezosurgery. Piezoelectric bone surgery or otherwise known as piezosurgery is a novel technique invented by Professor Vercellotti in 1988 to overcome the limitations of traditional instrumentation in oral bone surgery by modifying and improving conventional ultrasound technology. It is a promising, meticulous, and soft tissue sparing system for bone cutting based on low frequency ultrasonic microvibrations. The absence of macrovibration makes the instrument more manageable and allows greater intraoperative control with a significant increase in cutting safety in the more difficult anatomical cutting zone. The present review compares piezosurgery with the traditional tools and emphasizes on its mechanism of action, instruments, biologic effects, advantages, and limitations, as well as its various applications in the field of dentistry.