[Advantage of phase contrast for the identification of the cellular constituents of urinary sediment]. / Intéret du contraste de phase pour l'identification des constituants cellulaires du sédiment urinaire.

Phase contrast microscopic observation of the urinary sediment has to be performed on freshly collected urine. This technique, which permits an excellent definition of the cells and the maintenance of intra cellular motility, gives therefore a great accuracy in differential diagnosis, especially for little round cells. Among these, polynuclear granulocytes are easily recognized, because of their lobulated nuclei and of their numerous intra cytoplasmic motile granulations. Lymphocytes and lymphoblasts have round, regular, homogeneous nuclei. Renal tubular cells exhibit eccentric nuclei, with smudged chromatine, and round, refringent cytoplasmic vacuoles. Rapid and easy, this technic should be used for routine microscopic study or urinary sediments.

[Effect of sampling conditions on the concentration of corticosterone in rat brown fat]. / Influence des conditions de prélèvement sur la concentration en corticostérone dans la graisse brune de Rat

Corticosterone is more concentrated in the white and brown fat of the Rats killed after ether and nembutal anesthesia, than by decapitation. Corticosterone in the plasma increases in the same manner. There results show that adipose tissue is a pool of dilution in balance with the circulating corticosterone.

[Influence of the pituitary-adrenal axis on the brown fat tissue of the rat and golden hamster]. / Influence de l'axe hypophyso-surrénalien sur le tissu adipeux brun chez le rat et le hamster doré

The suppression or reduction of the adrenal secretion in the Rat and in the golden Hamster results in a reduction of the weight of brown adipose tissue and of the volume of intra cytoplasmic vacuoles. When corticotropin is given to normal and hypophysectomized animals, the intra cytoplasmic vacuolar system and the corticosterone concentration of brown fat increase. After sham hypophysectomy and short ether intake, a considerable lipidic depletion and a high level of corticosterone are observed. The authors speculate that the by adrenaline. The morphologic responses are much more intense and rapid lipid content of brown adipose tissue is increased by corticotropin and reduced by adrenaline. The morphologic responses are much more intense and rapid in brown adipose tissue than in white adipose tissue.

[Assay of cortisol and corticosterone in the plasma and adrenal glands of the golden hamster (Mesocricetus auratus)]. / Dosage du cortisol et de la corticostérone dans le plasma et les surrénales de Hamster doré (Mesocricetus auratus)

Corticosterone and cortisol are assayed by fluorometry after separation of the corticoids by chromatography on silica gel in the plasma and in the adrenals of golden Hamster brought up in the laboratory. The ratio cortisol : corticosterone is 2.7 in the plasma and 2.8 in the adrenals.