RESUMO
Conjugation of rabbit antibody with fluorescein isothiocyanate results in loss of the ability of that antibody to fix complement, mediate passive cutaneous anaphylaxis, or participate in cytophilic binding to macrophage surfaces. Fluorescein, perhaps because of molecular size or configuration, is a more potent suppressor of Fc function than are other reagents previously studied in this regard.
Assuntos
Anticorpos , Fluoresceínas , Fragmentos Fc das Imunoglobulinas , Animais , Reações Antígeno-Anticorpo , Sítios de Ligação de Anticorpos , Proteínas do Sistema Complemento , Eritrócitos/imunologia , Imunossupressores , Macrófagos/imunologia , Anafilaxia Cutânea Passiva , CoelhosRESUMO
The binding of cytophilic antibody to the macrophage surface results in patterns of distribution similar to those obtained when specific anti-macrophage antibody is used. These patterns are (a) diffuse circumferential binding at 4 degrees C, and (b) capping at 37 degrees C. The non-random distribution of the cytophilic antibody suggests that it binds to certain well-defined sites on the macrophage membrane.