RESUMO
A reversed-phase high-performance liquid chromatographic method with fluorescence detection for the determination of labile monomeric aluminium has been developed through pre-column complexation using morin as the analytical reagent. The highly fluorescent aluminium-morin complex (excitation wavelength 418 nm, emission wavelength 490 nm) was separated on a Spherisorb ODS 2 column with an eluent consisting of 30% methanol and 70% water (pH 1.0 with perchloric acid). The most remarkable point of this protocol was that only the most toxic aluminium species, that is, free aqua-aluminium ion and its monomeric hydroxo complex ions, selectively respond among various aluminium complexes. This strategy has been successfully applied to direct fractionation of the toxic aluminium in natural waters and biological samples without any pretreatment.
Assuntos
Alumínio/isolamento & purificação , Flavonoides/química , Poluentes Químicos da Água/isolamento & purificação , Alumínio/sangue , Alumínio/líquido cefalorraquidiano , Alumínio/urina , Cromatografia Líquida de Alta Pressão , Fluorescência , Água Doce/análise , Humanos , Indicadores e Reagentes/química , Sensibilidade e Especificidade , Chá/química , Poluentes Químicos da Água/sangue , Poluentes Químicos da Água/líquido cefalorraquidiano , Poluentes Químicos da Água/urinaRESUMO
Morin was used as a pre-column reagent for the determination of aluminum by RP-HPLC with fluorescence detection. This method has been successfully applied to direct determination of trace Al in environmental and biological samples. The response was linear from 6 x 10(-9) to 6 x 10(-5) M with a detection limit of 2 x 10(-9) M. In addition, the different Al complexes with morin were separated by the proposed HPLC procedure and their coordination ratios were depicted by molar-ratio method. The results showed that 1:1 and 2:1 Al-morin complexes formed.
