RESUMO
In algae-bacteria symbiotic systems, algae and bacteria work cooperatively to reduce aeration demand and carbon emissions during wastewater treatment. However, controlling and stabilizing microbial communities in the conventional algae-bacteria symbiotic systems are complicated and unstable. We, therefore, developed a novel sewage treatment system based on a filamentous algae-bacterial symbiotic granule system (AB) in a photo-sequencing batch reactor (PSBR). The exogenous signal molecules (i.e., N-acyl-homoserine lactones [AHLs]) were applied to promote the algae-bacteria consortia formation. The characteristics and performance of the mature algae-bacterial granular consortia were investigated and then compared with that of the activated sludge (AS) system under identical operating conditions. The chemical oxidation demand (COD) removal efficiencies were greater than 90% in the dynamic synergistic investigation, even without aeration. However, the addition of AHLs (5, 50, and 200 nmol/L) had a negligible effect on treatment performance. In static conditions, 10-9 nmol/L of AHLs can significantly regulate the algae-bacteria symbiotic system, and AHLs (<50 nmol/L) improved COD and NH4 + removal efficiencies by up to 90%. Moreover, the addition of AHLs augmented polysaccharide secretion but had little effect on protein secretion. In comparison to the AS system, the AB system demonstrated promise in terms of pollution removal efficiency and microbial diversity enhancement. PRACTITIONER POINTS: Oscillatoria can serve as an excellent biological carrier for immobilizing bacteria. AHLs (10-9 mol/L) regulated the operation state of algae-bacteria symbiotic system. AHLs altered the composition and content of EPS in the algae-bacteria system.
RESUMO
Sulfate-reducing bacteria (SRB) are a group of diverse anaerobic microorganisms omnipresent in natural habitats and engineered environments that use sulfur compounds as the electron acceptor for energy metabolism. Dissimilatory sulfate reduction (DSR)-based techniques mediated by SRB have been utilized in many sulfate-containing wastewater treatment systems worldwide, particularly for acid mine drainage, groundwater, sewage and industrial wastewater remediation. However, DSR processes are often operated suboptimally and disturbances are common in practical application. To improve the efficiency and robustness of SRB-based processes, it is necessary to study SRB metabolism and operational conditions. In this review, the mechanisms of DSR processes are reviewed and discussed focusing on intracellular and extracellular electron transfer with different electron donors (hydrogen, organics, methane and electrodes). Based on the understanding of the metabolism of SRB, responses of SRB to environmental stress (pH-, temperature-, and salinity-related stress) are summarized at the species and community levels. Application in these stressed conditions is discussed and future research is proposed. The feasibility of recovering energy and resources such as biohydrogen, hydrocarbons, polyhydroxyalkanoates, magnetite and metal sulfides through the use of SRB were investigated but some long-standing questions remain unanswered. Linking the existing scientific understanding and observations to practical application is the challenge as always for promotion of SRB-based techniques.
