Post-operation analgesic effects of flurbiprofen compound with sufentanil after maxillofacial surgery / 实用口腔医学杂志

Objective: To analyze the effects of different doses of flurbiprofen and other analgesic drugs on the postoperative analgesia in the Enhanced Recovery After Surgery (ERAS) . Methods: 98 patients with jaw cysts or multiple impact teeth were retrospectively analyzed. Patients were divided into 3 groups by the drug scheme: flurbiprofen 250 mg + 0. 9％ NS to 100 ml (group Ⅰ, n =32), sulfentanil 100 μg + azasetron 20 mg + 0. 9％ NS to 100 ml (group Ⅱ, n = 34), flurbiprofen 200 mg + sulfentanil 50 μg + azasetron 20 mg + 0. 9％ NS to 100 ml (group Ⅲ, n = 32) . Analgesic effects were evaluated at 3 h, 1 d and 2 d after surgery with Wong-Baker expression scale and numeric rating scale (NRS) . Questionnaire survey was also used to evaluate degree of satisfaction on analgesia. Results: Analgesia effective rate of group Ⅰ, Ⅱ and Ⅲ was 40. 62％, 67. 65％ and 68. 75％ respectively (P ＜0. 05) . Degree of satisfaction on analgesia of group Ⅰ, Ⅱ and Ⅲ was 71. 87％, 58. 82％ and 93. 75％ respectively (P ＜ 0. 05) .Conclusion: Flurbiprofen combined with sulfentanil has good analgesic effects and low side effects in the maxillofacial surgery of jaw cysts and multiple impact teeth.

Effect observation of low dose of docetaxel and cisplatin combined with radiotherapy on the elderly patients with esophageal cancer / 肿瘤研究与临床

Objective To investigate the efficacy and toxicity of low dose of docetaxel and cisplatin (TP) combined with radiotherapy in the treatment of elderly patients with esophageal cancer.Methods The data of 65 elderly patients with esophageal cancer were studied retrospectively, including 33 patients treated by TP combined with radiotherapy (chemoradiotherapy group) and 32 patients by radiotherapy only (radiotherapy group).Patients in both groups received 3D conventional radiotherapy (3D-CRT).In chemoradiotherapy group, 40 mg/1f docetaxel and 40 mg/1f cisplatin were administered once a week on the 1st, 8th, 15th, 22th, 36th day of five successive weeks.Results In chemoradiotherapy group and radiotherapy group, the response (CR+RR) rates were 87.8 ％ (29/33) and 65.6 ％ (21/32), respectively (P ＜ 0.05), the median TTPs were 5.5 months and 4.3 months, and the difference had statistically significant (P ＜ 0.05).The 1-year survival rate was 69.6 ％ and 59.3 ％ in chemoradiotherapy group and radiotherapy group, respectively, and there was no statistically significant difference between both groups (P ＞ 0.05).The incidences of esophagitis and gastrointestinal tract were slightly higher in chemoradiotherapy group than those in radiotherapy group (P ＜ 0.05).Conclusion Concurrent radiotherapy and chemotherapy with low dose TP can treat effectively esophageal cancer in elderly patients with the tolerable toxic reactions.

Application of Magnetic Resonance Imaging Fluid-attenuated Inversion Recovery Vascular Hyperintensities in Transient Ischemic Attack / 中国康复理论与实践

Objective To investigate the application of magnetic resonance imaging (MRI) fluid-attenuated inversion recovery vascular hyperintensities (FVH) for the diagnosis of transient ischemic attack (TIA). Methods Consecutive 218 inpatients for TIA from May 2011 to July 2013 were reviewed with gender, age, cardiovascular risk factors, TIA or a history of stroke, TIA duration, and calculate the ABCD2 scores. All patients accepted MRI and MRA within 24 hours of symptom onset. FVH positive patients would follow up MRI and MRA with-in 7 days. All the patients were followed up in 90 days. Results FVH was identified in 45 patients (21%), 15 cases of them was found diffu-sion weighted imaging (DWI) hyperintensities on the same sides. The prevalence of atrial fibrillation (AF, P<0.001), DWI positive (P=0.010) and arterial occlusive lesions (AOL, P=0.003) were more in the FVH positive patients than in the negative ones, while the duration of symptoms was shorter (P=0.010). Multivariate Logistic regression analysis showed that AF (OR=7.17, 95%CI:2.71-18.4), AOL (OR=4.93, 95%CI:3.53-12.6) and hemiplegic (OR=2.84, 95%CI:1.21-7.42) independently associated with FVH. 7 days after the onset, FVH was not found in 15 patients (65%, transient FVH), in whom the prevalence of AF was more (P=0.020), and AOL was less (P<0.001). A total of 197 patients (90%) were successfully followed up. In the FVH-positive patients, 5 cases developed into recurrent TIA and 6 into ischemic stroke (IS), focused on the the same sides of FVH;while the FVH-negative patients, 2 cases developed into recurrent TIA and 5 into IS. COX pro-portional hazard analysis showed that FVH (HR=3.64, 95%CI:1.08-12.6) and AOL (HR=3.82, 95%CI:1.07-15.8) independently associat-ed with the recurrence of TIA or IS. Conclusion FVH can be helpful for the diagnosis of TIA and predictions for recurrent TIA or IS after a TIA.

Application of Magnetic Resonance Imaging Fluid- attenuated Inversion Recovery Vascular Hyperintensities in Transient Ischemic Attack / 中国康复理论与实践

@#Objective To investigate the application of magnetic resonance imaging (MRI) fluid-attenuated inversion recovery vascular hyperintensities (FVH) for the diagnosis of transient ischemic attack (TIA). Methods Consecutive 218 inpatients for TIA from May 2011 to July 2013 were reviewed with gender, age, cardiovascular risk factors, TIA or a history of stroke, TIA duration, and calculate the ABCD2 scores. All patients accepted MRI and MRA within 24 hours of symptom onset. FVH positive patients would follow up MRI and MRA within 7 days. All the patients were followed up in 90 days. Results FVH was identified in 45 patients (21%), 15 cases of them was found diffusion weighted imaging (DWI) hyperintensities on the same sides. The prevalence of atrial fibrillation (AF, P<0.001), DWI positive (P= 0.010) and arterial occlusive lesions (AOL, P=0.003) were more in the FVH positive patients than in the negative ones, while the duration of symptoms was shorter (P=0.010). Multivariate Logistic regression analysis showed that AF (OR=7.17, 95% CI: 2.71-18.4), AOL (OR=4.93, 95% CI: 3.53-12.6) and hemiplegic (OR=2.84, 95% CI: 1.21-7.42) independently associated with FVH. 7 days after the onset, FVH was not found in 15 patients (65%, transient FVH), in whom the prevalence of AF was more (P=0.020), and AOL was less (P<0.001). A total of 197 patients (90%) were successfully followed up. In the FVH-positive patients, 5 cases developed into recurrent TIA and 6 into ischemic stroke (IS), focused on the the same sides of FVH; while the FVH-negative patients, 2 cases developed into recurrent TIA and 5 into IS. COX proportional hazard analysis showed that FVH (HR=3.64, 95% CI: 1.08-12.6) and AOL (HR=3.82, 95% CI: 1.07-15.8) independently associated with the recurrence of TIA or IS. Conclusion FVH can be helpful for the diagnosis of TIA and predictions for recurrent TIA or IS after a TIA.

Some leukocyte differentiation antigens express in side population cells in leukemia K562 cell line / 中国组织工程研究

BACKGROUND:To study the phenotypes of side population cells in leukemia is important for understanding the heterogeneity and origin of tumor cells, molecular markers and targeted therapy. OBJECTIVE:To identify whether the human chronic myeloid leukemia cellline-K562 contains side population cells or not, and to further observe the differences in expressions of leukocyte differentiation antigens from side population cellsubset and non-side population cells subset. METHODS:Flow cytometry was used to detect whether there were side population cells in the K562 celllines. Then, the expression of CD34+, CD34+CD38-, CD34+CD38+, HLA-DR+cells in the side population subsets and non-side population subsets. RESULTS AND CONCLUSION:Flow cytometry results showed that the K562 cellline contained side population cells, and the proportion of side population cells was much lower. The side population cells accounted for (2.7±0.5)%of viable cells in K562. The expressions of CD34+cells and CD34+CD38-cells in the side population subset were significantly higher than those in the non-side population subsets. The expressions of CD34+CD38+cells and HLA-DR+cells in the side population subset and non-side population subset did not have a significant difference. Heterogeneity was found in the differentiation antigen expression between the side population subset and non-side population subset.

脑转移瘤磁共振弥散加权像与原发肿瘤病理学相关性初步探讨 / 中国康复理论与实践

@#ObjectiveTo study the application of magnetic resonance imaging (MRI) diffusion-weighted imaging (DWI) in brain metastases, and the relationship between DWI results and the primary tumor pathology. Methods76 cases with brain metastases were grouped according to the primary cancer pathology. The apparent diffusion coefficient (ADC) of tumor parenchyma, capsule area, edema around tumors and normal parenchyma were determined; the ratio of high signal on the DWI were calculated (namely diffusion limited) in different tumor. ResultsThe ADC was (1.1364±0.310)×10-3 mm2/s in tumor parenchyma, (2.167±0.472)×10-3 mm2/s in capsule, (1.597±0.238)×10-3 mm2/s in edema and (0.812±0.158)×10-3 mm2/s in normal parenchyma (P<0.05). 15 cases appeared diffusion limited (19.7%), in which the primary tumors were lung cancer in 10 patients (66.6%) (including 5 cases with small cell lung cancer, 5 cases with none small cell lung cancer), breast cancer in 3 cases, and the other 2 cases were colon cancer and teratocarcinoma of testis (P>0.05). The minimum ADC were (0.72±0.16)×10-3 mm2/s in tumor parenchyma with diffuse limited metastases and (0.78±0.21)×10-3 mm2/s without diffuse limited (P=0.325). ConclusionDWI can respond to brain metastases to the microstructure change, but diffusion limited may not be related with the primary histopathology.

Mutations of the PIK3CA gene in diffuse large B cell lymphoma.

The PI3K/AKT pathway might be involved in the development of some certain diffuse large B-cell lymphoma (DLBCL) by as-yet unclear mechanisms. PIK3CA mutations in exons 9 and 20 were investigated in 76 primary human DLBCLs, 3 DLBCL cell lines (LY1, LY8, and LY10), and 9 related samples using polymerase chain reaction-based sequence analysis to assess the possible relevance of PIK3CA mutations in DLBCL to the PI3K/AKT pathway activation. AKT phosphorylation (pAKT) of 3 DLBCL cell lines and 76 primary DLBCL samples was also detected by Western blot and immunohistochemistry. All 3 cell lines showed high levels of pAKT, and 72.4% (55/76) of the DLBCLs expressed pAKT at various levels, indicating the activation of AKT. However, no mutation was found in exons 9 or 20 in PIK3CA in any of the 3 cell lines. Only 1 out of 76 primary DLBCLs (1.32%) harbored an exon 9 mutation, and no exon 20 mutation was detected. The case with mutations contained 3 mutation points. One was c.1634A>C resulting in E545A, which was in a previously reported hotspot. The other 2 were novel c.1658G>C and c.1659delT frameshift mutations. We conclude that the PI3K/AKT pathway is activated in DLBCL and that PIK3CA is rarely mutated in DLBCL, indicating there could be some other PI3K-pathway activation mechanisms operative in DLBCL.