Assuntos
Proteínas de Ligação a DNA/genética , Proteínas de Drosophila , Drosophila melanogaster/genética , Proteínas de Insetos/genética , Proteínas Nucleares/genética , Alelos , Animais , Elementos Facilitadores Genéticos , Expressão Gênica , Genes de Insetos , Mutação , Fenótipo , Regiões Promotoras Genéticas , Proteínas RepressorasRESUMO
Method in situ hybridization was used to localize 5S rRNA genes on human chromosomes. It was shown that 5S genes are situated on the 1-st chromosome, positions q42.11-42.13 and q 43. The genome organization of 5S rRNA gene cluster was tested by blot-hybridization of fibroblast DNA restriction fragments separated by electrophoresis. It was shown that cluster comprises of repeats with different length and restriction site specificity. The size of HindIII individual repeats-2.6 kb, BamHI-repeats-2.3 and 1.8 kb. The most part of 5S repeats were organized in oligomers. Long 5S rDNA fragments (100 kb) in which BamHI, HindIII, and AccI restriction sites are absent were demonstrated. The results obtained allow to suggest the multicluster organization of human 5S rRNA genes, these clusters are localized in a short distance from each other on the long arm of 1-st chromosome.
Assuntos
Genoma Humano , Família Multigênica , RNA Ribossômico 5S/genética , Southern Blotting , Cromossomos Humanos Par 1 , DNA , Enzimas de Restrição do DNA , Humanos , Hibridização In SituRESUMO
The method of dot DNA-DNA hybridization was used to reveal the inhibition of the synthesis of the adenoviral DNA by 6-azacytidine, cyclocytidine and ribamidyl in the adenovirus-infected cells Hep-2, a degree of which depended on the preparation concentration.
Assuntos
Infecções por Adenoviridae/metabolismo , Infecções por Adenovirus Humanos/metabolismo , DNA Viral/análise , Nucleosídeos/farmacologia , Ancitabina/farmacologia , Azacitidina/farmacologia , Células Cultivadas/efeitos dos fármacos , Células Cultivadas/metabolismo , DNA Viral/biossíntese , Humanos , Isomerismo , Hibridização de Ácido Nucleico , Ribavirina/farmacologiaRESUMO
Certain regularities in content and organization of pyrimidine oligonucleotide sequences of DNA from 15 insect species belonging to 4 orders were studied. The degree of nucleotide clusterization in insect DNA was found to be species-specific, being the highest in Hymenoptera and lowest in Lepidoptera; the Blattodea and Coleoptera occupy an intermediate position by this index between them. The changes in the DNA cluster structure during the evolution of insect species are not of vector type; the degree of clusterization of DNA nucleotide is either increased (Hymenoptera) or decreased (Lepidoptera as compared with Blattodea). In the DNA oligonucleotide fractions containing both pyrimidine nucleotides the percentage content of thymidyl nucleotides is much higher than that of cytidyl nucleotides, the thymine content being increased with the lengthening of oligopyrimidine clusters. The insect species with a higher degree of clusterization of DNA pyrimidine nucleotides contain more thymidyl nucleotide residues. These results agree well with the hypothesis suggesting that during the evolution of large taxons the accumulation of long pyrimidine sequences in animal DNA is accompanied by an increase of thymidyl nucleotide content in them. This can largely be due to the increase of matrix resistance during the evolution and is biologically significant for animals of any taxons, including insects.
