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1.
J Am Soc Mass Spectrom ; 35(6): 1076-1088, 2024 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-38660944

RESUMO

A recently developed proteolytic reactor, designed for protein structural investigation, was coupled to ion mobility mass spectrometry to monitor collisional cross section (CCS) evolution of model proteins undergoing trypsin-mediated mono enzymatic digestion. As peptides are released during digestion, the CCS of the remaining protein structure may deviate from the classical 2/3 power of the CCS-mass relationship for spherical structures. The classical relationship between CCS and mass (CCS = A × M2/3) for spherical structures, assuming a globular shape in the gas phase, may deviate as stabilizing elements are lost during digestion. In addition, collision-induced unfolding (CIU) experiments on partially digested proteins provided insights into the CCS resilience in the gas phase to ion activation, potentially due to the presence of stabilizing elements. The study initially investigated a model peptide ModBea (3 kDa), assessing the impact of disulfide bridges on CCS resilience in both reduced and oxidized forms. Subsequently, ß-lactoglobulin (2 disulfide bridges), calmodulin (Ca2+ coordination cation), and cytochrome c (heme) were selected to investigate the influence of common structuring elements on CCS resilience. CIU experiments probed the unfolding process, evaluating the effect of losing specific peptides on the energy landscapes of partially digested proteins. Comparisons of the TWCCSN2→He to trend curves describing the CCS/mass relationship revealed that proteins with structure-stabilizing elements consistently exhibit TWCCSN2→He and greater resilience toward CIU compared to proteins lacking these elements. The integration of online digestion, ion mobility, and CIU provides a valuable tool for identifying structuring elements in biopolymers in the gas phase.


Assuntos
Calmodulina , Espectrometria de Mobilidade Iônica , Desdobramento de Proteína , Proteínas , Espectrometria de Mobilidade Iônica/métodos , Proteínas/química , Calmodulina/química , Calmodulina/metabolismo , Lactoglobulinas/química , Lactoglobulinas/metabolismo , Citocromos c/química , Citocromos c/análise , Espectrometria de Massas/métodos , Peptídeos/química , Peptídeos/análise , Tripsina/química , Tripsina/metabolismo , Animais , Conformação Proteica
2.
Nat Commun ; 14(1): 1881, 2023 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-37019903

RESUMO

Calreticulin (CALR) frameshift mutations represent the second cause of myeloproliferative neoplasms (MPN). In healthy cells, CALR transiently and non-specifically interacts with immature N-glycosylated proteins through its N-terminal domain. Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation. Here, we identify the basis of the acquired specificity of CALR mutants for TpoR and define the mechanisms by which complex formation triggers TpoR dimerization and activation. Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR. We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR. Finally, we propose a model of the tetrameric TpoR-CALR mutant complex and identify potentially targetable sites.


Assuntos
Calreticulina , Transtornos Mieloproliferativos , Humanos , Dimerização , Calreticulina/metabolismo , Receptores de Trombopoetina/metabolismo , Mutação da Fase de Leitura , Transtornos Mieloproliferativos/genética , Mutação , Janus Quinase 2/metabolismo
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